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Protein

Tetraacyldisaccharide 4'-kinase

Gene

lpxK

Organism
Methylobacterium sp. (strain 4-46)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Transfers the gamma-phosphate of ATP to the 4'-position of a tetraacyldisaccharide 1-phosphate intermediate (termed DS-1-P) to form tetraacyldisaccharide 1,4'-bis-phosphate (lipid IVA).UniRule annotation

Catalytic activityi

ATP + (2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl phosphate) = ADP + (2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-4-O-phospho-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl phosphate).UniRule annotation

Pathwayi: lipid IV(A) biosynthesis

This protein is involved in step 6 of the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA)
  2. UDP-3-O-acyl-N-acetylglucosamine deacetylase (M446_3203), UDP-3-O-acyl-N-acetylglucosamine deacetylase (lpxC)
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. Lipid-A-disaccharide synthase (lpxB)
  6. Tetraacyldisaccharide 4'-kinase (lpxK)
This subpathway is part of the pathway lipid IV(A) biosynthesis, which is itself part of Glycolipid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine, the pathway lipid IV(A) biosynthesis and in Glycolipid biosynthesis.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi52 – 59ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Lipid A biosynthesis, Lipid biosynthesis, Lipid metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00359; UER00482.

Names & Taxonomyi

Protein namesi
Recommended name:
Tetraacyldisaccharide 4'-kinaseUniRule annotation (EC:2.7.1.130UniRule annotation)
Alternative name(s):
Lipid A 4'-kinaseUniRule annotation
Gene namesi
Name:lpxKUniRule annotation
Ordered Locus Names:M446_6829
OrganismiMethylobacterium sp. (strain 4-46)
Taxonomic identifieri426117 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium
Proteomesi
  • UP000001185 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001347481 – 332Tetraacyldisaccharide 4'-kinaseAdd BLAST332

Interactioni

Protein-protein interaction databases

STRINGi426117.M446_6829.

Family & Domainsi

Sequence similaritiesi

Belongs to the LpxK family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CHD. Bacteria.
COG1663. LUCA.
HOGENOMiHOG000004954.
KOiK00912.
OMAiPTVIYLI.
OrthoDBiPOG091H000L.

Family and domain databases

HAMAPiMF_00409. LpxK. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR003758. Tetraacyldisaccharide_4-kinase.
[Graphical view]
PfamiPF02606. LpxK. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00682. lpxK. 1 hit.

Sequencei

Sequence statusi: Complete.

B0UJ62-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRAPDFWWRD PPSLPARLLA PVGALYGAAA ARRMARPGAP APCPVVCVGN
60 70 80 90 100
VTLGGAGKTP TALALAALLR ELGARPAFLS RGYGGSLPGP LRVDPAAHGA
110 120 130 140 150
AEVGDEPLLI ARAAPTIVAR DRPAGAAACA AAGADVIVMD DGLQNPSLRK
160 170 180 190 200
DWSLAVFDAG VGIGNGLPFP AGPLRAPLGA QWPLVSAVLV IGDAAQGRPL
210 220 230 240 250
LAAAAARGLP ALRGRLAPDA AAAAALRGRP VLAFAGIGRP DKFFESLRAV
260 270 280 290 300
GAELRGTRAF PDHHAYRAGE IAALEREATR RGLTLVTTEK DRVRLPAAMA
310 320 330
VAVLPVRLAL APDDAALLRA RLAALLRGRA GP
Length:332
Mass (Da):33,859
Last modified:April 8, 2008 - v1
Checksum:i3D98AF3F80CAA53E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000943 Genomic DNA. Translation: ACA21074.1.
RefSeqiWP_012336448.1. NC_010511.1.

Genome annotation databases

EnsemblBacteriaiACA21074; ACA21074; M446_6829.
KEGGimet:M446_6829.
PATRICi22594962. VBIMetSp32184_6776.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000943 Genomic DNA. Translation: ACA21074.1.
RefSeqiWP_012336448.1. NC_010511.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi426117.M446_6829.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACA21074; ACA21074; M446_6829.
KEGGimet:M446_6829.
PATRICi22594962. VBIMetSp32184_6776.

Phylogenomic databases

eggNOGiENOG4105CHD. Bacteria.
COG1663. LUCA.
HOGENOMiHOG000004954.
KOiK00912.
OMAiPTVIYLI.
OrthoDBiPOG091H000L.

Enzyme and pathway databases

UniPathwayiUPA00359; UER00482.

Family and domain databases

HAMAPiMF_00409. LpxK. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR003758. Tetraacyldisaccharide_4-kinase.
[Graphical view]
PfamiPF02606. LpxK. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00682. lpxK. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiLPXK_METS4
AccessioniPrimary (citable) accession number: B0UJ62
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: April 8, 2008
Last modified: November 2, 2016
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.