B0UIR7 (B0UIR7_METS4) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
DNA topoisomerase RuleBase RU000518
EC=5.99.1.2 RuleBase RU000518

Gene names

Ordered Locus Names:

M446_4350 EMBL ACA18693.1

Organism

Methylobacterium sp. (strain 4-46) [Complete proteome] [HAMAP] EMBL ACA18693.1

Taxonomic identifier

426117 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Methylobacteriaceae › Methylobacterium

Protein attributes

Sequence length	913 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS023406
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. RuleBase RU000518
Sequence similarities	Belongs to the type IA topoisomerase family. RuleBase RU004206 Contains 1 Toprim domain. SAAS SAAS005733

Ontologies

Keywords
Ligand	ATP-binding SAAS SAAS005733 DNA-binding RuleBase RU000518 SAAS SAAS005733 Magnesium SAAS SAAS023406 Metal-binding SAAS SAAS023406 Nucleotide-binding
Molecular function	Isomerase Topoisomerase RuleBase RU000518 SAAS SAAS005733
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular_component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	B0UIR7 [UniParc]. Last modified April 8, 2008. Version 1. Checksum: 0E74AB4492FDE7E2	FASTA	913	99,264
	10 20 30 40 50 60 MKVVVVESPA KAKTINKYLG HDYEVLASFG HVRDLPAKDG SVDPEQDFKM LWELEERGAK 70 80 90 100 110 120 RVSEIARAVK GADTLILATD PDREGEAISW HVLEALQAKR VLKDVAVERV TFNAITKDAV 130 140 150 160 170 180 QTAMANPRQI DQALVDAYLA RRALDYLVGF NLSPVLWRKL PGARSAGRVQ SVALRLVCDR 190 200 210 220 230 240 EAEIEAFKPR EYWSLVATLA TAKGATFEAR LVGADGKRIQ RLDVGTAEEA EAFRRDLELA 250 260 270 280 290 300 TFAVASVESK PAKRHPQPPF TTSTLQQEAS RKLGLAPAQT MRIAQRLYEG VEIGGETVGL 310 320 330 340 350 360 ITYMRTDGVD MAPEAIRDAR RVIGAEFGDA YLPGAPRSYS VKAKNAQEAH EAVRPTDLGR 370 380 390 400 410 420 LPKEVARFLE PEQAKLYELI WIRTIASQME SAELERTVVE IAAQVGPRRL DLRATGQVVR 430 440 450 460 470 480 FDGFLTLYQE GKDDEEDEES RRLPPMQAGD PLRRERVAAT QHFTEPPPRY SEASLVKRME 490 500 510 520 530 540 ELGIGRPSTY AAVLQVLRDR EYVRLDKKRL VPEDKGRLVT GFLESFFQRY VEYDFTADLE 550 560 570 580 590 600 EQLDRISNAE IDWREVLRDF WRDFSAALAG TKELRTAEVL EALNQLLGPH IFPPRADGAN 610 620 630 640 650 660 PRACPNCAGG TLSLKLGKFG AFVGCSNYPE CKYTRQLSAA GLDGDGEGSA GEGGQPGVRV 670 680 690 700 710 720 LGEDPATGLP VTLRDGRFGP FVQLGEASAE KGAEKPKRSS LPRGLTPADV TLETALKLLA 730 740 750 760 770 780 LPREVARHPE SGEPILVNLG RFGPYVQHGK TYANLGKDDD LLEIGANRAI DLIVAKEQGG 790 800 810 820 830 840 GRPAADPGRV LGNDPEGRAV TVKAGRYGPY VTDGEVNATL PKGADKEALT LEEALRLIVA 850 860 870 880 890 900 RREAGGGTKK KTSGRARAAR KTEAKPAAKA KAEKAAKPAA AGKSASAGKS ASAAKSATAP 910 EKAPARKKAA AAE

References

[1]

"Complete sequence of chromosome of Methylobacterium sp. 4-46." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Ivanova N., Marx C.J., Richardson P. Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 4-46 EMBL ACA18693.1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000943 Genomic DNA. Translation: ACA18693.1.
RefSeq	YP_001771127.1. NC_010511.1.
3D structure databases
ProteinModelPortal	B0UIR7.
ModBase	Search...
Protein-protein interaction databases
STRING	426117.M446_4350.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACA18693; ACA18693; M446_4350.
GeneID	6134697.
KEGG	met:M446_4350.
PATRIC	22589848. VBIMetSp32184_4257.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1754.
HOGENOM	HOG000004020.
KO	K03168.
OMA	GKYGPYL.
ProtClustDB	PRK07561.
Enzyme and pathway databases
BioCyc	MSP426117:GI2I-4392-MONOMER.
Family and domain databases
Gene3D	1.10.460.10. 2 hits. 2.70.20.10. 2 hits.
InterPro	IPR000380. Topo_IA. IPR003601. Topo_IA_2. IPR023406. Topo_IA_AS. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013825. Topo_IA_cen_sub2. IPR023405. Topo_IA_core_domain. IPR003602. Topo_IA_DNA-bd. IPR013498. Topo_IA_Znf. IPR005733. TopoI_bac-type. IPR006171. Toprim_domain. [Graphical view]
PANTHER	PTHR11390. PTHR11390. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. PF01396. zf-C4_Topoisom. 1 hit. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
SUPFAM	SSF56712. Topo_IA_core. 1 hit.
TIGRFAMs	TIGR01051. topA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B0UIR7_METS4

Accession

Primary (citable) accession number: B0UIR7

Entry history

Integrated into UniProtKB/TrEMBL:	April 8, 2008
Last sequence update:	April 8, 2008
Last modified:	May 1, 2013
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)