B0UI57 (B0UI57_METS4) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Cobyrinic acid A,C-diamide synthase HAMAP-Rule MF_00027

Gene names

Name:	cobB HAMAP-Rule MF_00027
Ordered Locus Names:	M446_2455 EMBL ACA16908.1

Organism

Methylobacterium sp. (strain 4-46) [Complete proteome] [HAMAP] EMBL ACA16908.1

Taxonomic identifier

426117 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Methylobacteriaceae › Methylobacterium

Protein attributes

Sequence length	447 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Responsible for the amidation of carboxylic groups at position A and C of either cobyrinic acid or hydrogenobrynic acid. NH2 groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation By similarity. HAMAP-Rule MF_00027
Pathway	Cofactor biosynthesis; adenosylcobalamin biosynthesis. HAMAP-Rule MF_00027
Sequence similarities	Belongs to the CobB/CobQ family. CobB subfamily. HAMAP-Rule MF_00027 Contains 1 GATase cobBQ-type domain. HAMAP-Rule MF_00027 SAAS SAAS011698

Ontologies

Keywords
Biological process	Cobalamin biosynthesis HAMAP-Rule MF_00027 SAAS SAAS011698
Domain	Glutamine amidotransferase HAMAP-Rule MF_00027 SAAS SAAS011698
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cobalamin biosynthetic process Inferred from electronic annotation. Source: HAMAP glutamine metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	cobalamin-transporting ATPase activity Inferred from electronic annotation. Source: HAMAP cobyrinic acid a,c-diamide synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	337	1	Nucleophile By similarity HAMAP-Rule MF_00027
	Active site	442	1	By similarity HAMAP-Rule MF_00027

Sequences

	Sequence		Length	Mass (Da)
	B0UI57 [UniParc]. Last modified April 8, 2008. Version 1. Checksum: 96B998C6C86E0803	FASTA	447	45,901
	10 20 30 40 50 60 MTDLSACGPL PTRGLLIAAP RSGSGKTTVT LAILRALARR GLAVAGAKCG PDYIDPAFHA 70 80 90 100 110 120 AATGRPSYNL DSFAMDDDLL DAVAARAAAG ADLLVAEGSM GLFDGVVAET GRSGANADVA 130 140 150 160 170 180 ARYGWPVVLV LDVSGAAQSA AAVALGCRLY DPRLAFAGVI LNKVASPRHR RLVEAGLARA 190 200 210 220 230 240 GLPVLGAIGR DAALVLPERH LGLVQAEETG DLAARLDRLA ALAEASLDLD ALLRAAGGRV 250 260 270 280 290 300 PASGRHRLPD PPGQRIALAR DAAFSFVYPH MTAGWRAAGA ELVPFSPLAD EPPPPDCDAC 310 320 330 340 350 360 WLPGGYPELH AGRLAAAGRF LAGLRAFARA RPVHGECGGY MVLGEGLEDA GGHRHAMAGL 370 380 390 400 410 420 LPVATSYARR KLHLGYRLAA LLGDGPLGRA GDTVVGHEFH YASELTPAAD AACGLARVSD 430 440 GEGRSLGIAG HRLGRVSGSF FHLIGGR

References

[1]

"Complete sequence of chromosome of Methylobacterium sp. 4-46." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Ivanova N., Marx C.J., Richardson P. Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 4-46 EMBL ACA16908.1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000943 Genomic DNA. Translation: ACA16908.1.
RefSeq	YP_001769342.1. NC_010511.1.
3D structure databases
ProteinModelPortal	B0UI57.
ModBase	Search...
Protein-protein interaction databases
STRING	426117.M446_2455.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACA16908; ACA16908; M446_2455.
GeneID	6129937.
KEGG	met:M446_2455.
PATRIC	22586160. VBIMetSp32184_2440.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1797.
HOGENOM	HOG000289959.
KO	K02224.
OMA	CWLPGGY.
ProtClustDB	PRK01077.
Enzyme and pathway databases
BioCyc	MSP426117:GI2I-2468-MONOMER.
UniPathway	UPA00148.
Family and domain databases
HAMAP	MF_00027. CobB.
InterPro	IPR004484. CbiA_synth. IPR017929. CobB/CobQ_GATase. IPR002586. CobQ/CobB/MinD/ParA_Nub-bd_dom. IPR011698. GATase_3. [Graphical view]
Pfam	PF01656. CbiA. 1 hit. PF07685. GATase_3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00379. cobB. 1 hit.
PROSITE	PS51274. GATASE_COBBQ. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B0UI57_METS4

Accession

Primary (citable) accession number: B0UI57

Entry history

Integrated into UniProtKB/TrEMBL:	April 8, 2008
Last sequence update:	April 8, 2008
Last modified:	May 1, 2013
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)