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B0UEX5

- PNP_METS4

UniProt

B0UEX5 - PNP_METS4

Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Methylobacterium sp. (strain 4-46)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 44 (01 Oct 2014)
      Sequence version 1 (08 Apr 2008)
      Previous versions | rss
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    Functioni

    Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

    Catalytic activityi

    RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi487 – 4871MagnesiumUniRule annotation
    Metal bindingi493 – 4931MagnesiumUniRule annotation

    GO - Molecular functioni

    1. 3'-5'-exoribonuclease activity Source: InterPro
    2. magnesium ion binding Source: UniProtKB-HAMAP
    3. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
    4. RNA binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. mRNA catabolic process Source: UniProtKB-HAMAP
    2. RNA processing Source: InterPro

    Keywords - Molecular functioni

    Nucleotidyltransferase, Transferase

    Keywords - Ligandi

    Magnesium, Metal-binding, RNA-binding

    Enzyme and pathway databases

    BioCyciMSP426117:GI2I-4969-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
    Alternative name(s):
    Polynucleotide phosphorylaseUniRule annotation
    Short name:
    PNPaseUniRule annotation
    Gene namesi
    Name:pnpUniRule annotation
    Ordered Locus Names:M446_4916
    OrganismiMethylobacterium sp. (strain 4-46)
    Taxonomic identifieri426117 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium
    ProteomesiUP000001185: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 725725Polyribonucleotide nucleotidyltransferasePRO_1000147932Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi426117.M446_4916.

    Structurei

    3D structure databases

    ProteinModelPortaliB0UEX5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini554 – 61360KHUniRule annotationAdd
    BLAST
    Domaini623 – 69169S1 motifUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
    Contains 1 KH domain.UniRule annotation
    Contains 1 S1 motif domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1185.
    HOGENOMiHOG000218327.
    KOiK00962.
    OMAiHGNFKSN.
    OrthoDBiEOG6WT8CC.

    Family and domain databases

    Gene3Di1.10.10.400. 1 hit.
    2.40.50.140. 1 hit.
    3.30.1370.10. 1 hit.
    3.30.230.70. 2 hits.
    HAMAPiMF_01595. PNPase.
    InterProiIPR001247. ExoRNase_PH_dom1.
    IPR015847. ExoRNase_PH_dom2.
    IPR004087. KH_dom.
    IPR004088. KH_dom_type_1.
    IPR012340. NA-bd_OB-fold.
    IPR012162. PNPase.
    IPR027408. PNPase/RNase_PH_dom.
    IPR015848. PNPase_PH_RNA-bd_bac/org-type.
    IPR003029. Rbsml_prot_S1_RNA-bd_dom.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR022967. RNA-binding_domain_S1.
    [Graphical view]
    PANTHERiPTHR11252. PTHR11252. 1 hit.
    PfamiPF00013. KH_1. 1 hit.
    PF03726. PNPase. 1 hit.
    PF01138. RNase_PH. 2 hits.
    PF03725. RNase_PH_C. 2 hits.
    PF00575. S1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005499. PNPase. 1 hit.
    SMARTiSM00322. KH. 1 hit.
    SM00316. S1. 1 hit.
    [Graphical view]
    SUPFAMiSSF50249. SSF50249. 1 hit.
    SSF54211. SSF54211. 2 hits.
    SSF54791. SSF54791. 1 hit.
    SSF55666. SSF55666. 2 hits.
    TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
    PROSITEiPS50084. KH_TYPE_1. 1 hit.
    PS50126. S1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B0UEX5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFDTQREELL WGGRKLVLET GKVARQADGA VVASYGETTV LATVVSMKEP    50
    KPGIDFLPLT VNYQERAYAA GRIPGGYFKR EGRPSEKETL VSRLIDRPIR 100
    PLFVEGWRND TQVVVTVLSH DLENDPDIVS MVAASAALTL SGVPFMGPIG 150
    AARVGYLGGQ YKLNPTVQEM EESSLDLVVA GTDAAVLMVE SEAKELPEDV 200
    MLGAVMFGHK HFQPVIEAII RLAEKAAKEP RDFQPTDLTE VEKAVLEIGE 250
    ADLREAYRKT VKQERYAAVD AVKAKVMAAL VPEGGEAKFE PEKVKAAFKE 300
    VQAKVVRWNI LDTGSRIDGR DVRTVRPILS EVGVLPRAHG SALFTRGETQ 350
    ALVVATLGTG DDEQFIDALE GTYKETFLLH YNFPPYSVGE TGRMGSPGRR 400
    EIGHGKLAWR AVHPLLPPAH EFPYTLRVVS EITESNGSSS MATVCGSSLA 450
    LMDAGVPLRR PVAGIAMGLI LEGERYAVLS DILGDEDHLG DMDFKVAGTE 500
    EGVTSLQMDI KIAGITEEIM RVALDQAKEG RAHILGEMAK ALTAARPELG 550
    EHAPRIETMQ IPTDKIREVI GTGGKVIREI VEKTGAKIDI QDTGVVKIAS 600
    SDGKAIKAAY NWIRSIVAEP EAGMIYDGTV VKTMEFGAFV NFFGAKDGLV 650
    HISELAPQRV AKVTDVVKEG DKVKVKFLGQ DERGKIRLSM KVVDQQTGED 700
    LTEKLKAERE ADRNRERQAR QSAGE 725
    Length:725
    Mass (Da):79,004
    Last modified:April 8, 2008 - v1
    Checksum:i20F838835B5ABBCB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000943 Genomic DNA. Translation: ACA19245.1.
    RefSeqiYP_001771679.1. NC_010511.1.

    Genome annotation databases

    EnsemblBacteriaiACA19245; ACA19245; M446_4916.
    GeneIDi6135556.
    KEGGimet:M446_4916.
    PATRICi22591026. VBIMetSp32184_4835.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000943 Genomic DNA. Translation: ACA19245.1 .
    RefSeqi YP_001771679.1. NC_010511.1.

    3D structure databases

    ProteinModelPortali B0UEX5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 426117.M446_4916.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACA19245 ; ACA19245 ; M446_4916 .
    GeneIDi 6135556.
    KEGGi met:M446_4916.
    PATRICi 22591026. VBIMetSp32184_4835.

    Phylogenomic databases

    eggNOGi COG1185.
    HOGENOMi HOG000218327.
    KOi K00962.
    OMAi HGNFKSN.
    OrthoDBi EOG6WT8CC.

    Enzyme and pathway databases

    BioCyci MSP426117:GI2I-4969-MONOMER.

    Family and domain databases

    Gene3Di 1.10.10.400. 1 hit.
    2.40.50.140. 1 hit.
    3.30.1370.10. 1 hit.
    3.30.230.70. 2 hits.
    HAMAPi MF_01595. PNPase.
    InterProi IPR001247. ExoRNase_PH_dom1.
    IPR015847. ExoRNase_PH_dom2.
    IPR004087. KH_dom.
    IPR004088. KH_dom_type_1.
    IPR012340. NA-bd_OB-fold.
    IPR012162. PNPase.
    IPR027408. PNPase/RNase_PH_dom.
    IPR015848. PNPase_PH_RNA-bd_bac/org-type.
    IPR003029. Rbsml_prot_S1_RNA-bd_dom.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR022967. RNA-binding_domain_S1.
    [Graphical view ]
    PANTHERi PTHR11252. PTHR11252. 1 hit.
    Pfami PF00013. KH_1. 1 hit.
    PF03726. PNPase. 1 hit.
    PF01138. RNase_PH. 2 hits.
    PF03725. RNase_PH_C. 2 hits.
    PF00575. S1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005499. PNPase. 1 hit.
    SMARTi SM00322. KH. 1 hit.
    SM00316. S1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50249. SSF50249. 1 hit.
    SSF54211. SSF54211. 2 hits.
    SSF54791. SSF54791. 1 hit.
    SSF55666. SSF55666. 2 hits.
    TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
    PROSITEi PS50084. KH_TYPE_1. 1 hit.
    PS50126. S1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 4-46.

    Entry informationi

    Entry nameiPNP_METS4
    AccessioniPrimary (citable) accession number: B0UEX5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 14, 2009
    Last sequence update: April 8, 2008
    Last modified: October 1, 2014
    This is version 44 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3