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B0UCB6

- NAPA_METS4

UniProt

B0UCB6 - NAPA_METS4

Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Methylobacterium sp. (strain 4-46)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 51 (01 Oct 2014)
      Sequence version 1 (08 Apr 2008)
      Previous versions | rss
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    Functioni

    Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.UniRule annotation

    Catalytic activityi

    Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster.UniRule annotation
    Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi47 – 471Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi50 – 501Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi54 – 541Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi82 – 821Iron-sulfur (4Fe-4S)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. electron carrier activity Source: UniProtKB-HAMAP
    3. iron ion binding Source: UniProtKB-HAMAP
    4. molybdenum ion binding Source: InterPro
    5. nitrate reductase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
    2. nitrate assimilation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Nitrate assimilation, Transport

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

    Enzyme and pathway databases

    BioCyciMSP426117:GI2I-1027-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Periplasmic nitrate reductaseUniRule annotation (EC:1.7.99.4UniRule annotation)
    Gene namesi
    Name:napAUniRule annotation
    Ordered Locus Names:M446_1019
    OrganismiMethylobacterium sp. (strain 4-46)
    Taxonomic identifieri426117 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium
    ProteomesiUP000001185: Chromosome

    Subcellular locationi

    Periplasm UniRule annotation

    GO - Cellular componenti

    1. periplasmic space Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Periplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2929Tat-type signalUniRule annotationAdd
    BLAST
    Chaini30 – 838809Periplasmic nitrate reductasePRO_5000309931Add
    BLAST

    Post-translational modificationi

    Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

    Interactioni

    Subunit structurei

    Interacts with NapB.UniRule annotation

    Protein-protein interaction databases

    STRINGi426117.M446_1019.

    Structurei

    3D structure databases

    ProteinModelPortaliB0UCB6.
    SMRiB0UCB6. Positions 40-827.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini40 – 96574Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation
    Contains 1 4Fe-4S Mo/W bis-MGD-type domain.UniRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG0243.
    HOGENOMiHOG000031441.
    KOiK02567.
    OMAiHQLVEAP.
    OrthoDBiEOG6CVV7G.

    Family and domain databases

    HAMAPiMF_01630. Nitrate_reduct.
    InterProiIPR009010. Asp_de-COase-like_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR027467. MopterinOxRdtase_cofactor_BS.
    IPR010051. Periplasm_NO3_reductase_lsu.
    IPR006311. TAT_signal.
    IPR019546. TAT_signal_bac_arc.
    [Graphical view]
    PfamiPF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    [Graphical view]
    SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view]
    SUPFAMiSSF50692. SSF50692. 1 hit.
    TIGRFAMsiTIGR01706. NAPA. 1 hit.
    TIGR01409. TAT_signal_seq. 1 hit.
    PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
    PS51318. TAT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    B0UCB6-1 [UniParc]FASTAAdd to Basket

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    MDMSRRTLLK AQAAAAAAAV AGIDLPAEAQ NLAAGEDIAL KWSKAPCRFC    50
    GTGCGVMVGV KDGRVVATHG DMQAEVNRGL NCVKGYFLSK IMYGADRLTQ 100
    PLMRVRGGKF DKNAELQPVS WDQAFDEMAR QWKRVLREKG PKAVGMFGSG 150
    QWTIFEGYAA TKLMRAGFRS NNLDPNARHC MASAAVGFIR TFGMDEPMGC 200
    YDDFEHADAF VLWGSNMAEM HPILWTRVID RRLSAPHVRI ATLSTYEHRT 250
    TELSDQSLIF QPGTDLAILN FVANYIIQNG AVNRDFVERH VNFRVANTDI 300
    GYGLRPEHVL EQRATHANEA AVSQPSNFDA YARMVGEYTL EKTEELTGVS 350
    KERLLTLAKT YADPKIRVMS LWTMGFNQHV RGVWANHLVY NIHLLTGKIS 400
    EPGNSPFSLT GQPSACGTAR EVGTFSHRLP ADMQVNNPEH RKHAEEIWKL 450
    PPGLLNGEIG YHAVQQDRML KDGKLNAYWI MCNNNLQTAP NTNTETYPGY 500
    RNPANFVVCS DPYPTVTAMA ADVVLPTAMW VEKEGAYGNA ERRTHMWRQL 550
    VNAPGDARSD LWQLMEFSKR FTTDEVWAQE ILVAHPDYKG KSLFDVLWRN 600
    GSVDRFGLDE IAADHENFES KQFGFYVQKG LFEEYASFGR GHGHDLAPFD 650
    RYHEVRGLRW PVVDGKETRW RYREGHDPYV EKGAGWQFYG NPDRRAVVMA 700
    APYEPPAEAP DQEYDLWLVT GRVLEHWHSG SMTMRVPELY RAFTGAVMFM 750
    HPDDAQKRGL RRGQEVRIIS RRGEIRSRVE TRGRNKMPPG RVFVPFFDAS 800
    QLINKATLDA TDPISKQTDF KKCAVKVIGV ATAERGAE 838
    Length:838
    Mass (Da):94,400
    Last modified:April 8, 2008 - v1
    Checksum:i11DFA43229E04111
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000943 Genomic DNA. Translation: ACA15550.1.
    RefSeqiWP_012330967.1. NC_010511.1.
    YP_001767984.1. NC_010511.1.

    Genome annotation databases

    EnsemblBacteriaiACA15550; ACA15550; M446_1019.
    GeneIDi6129780.
    KEGGimet:M446_1019.
    PATRICi22583391. VBIMetSp32184_1062.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000943 Genomic DNA. Translation: ACA15550.1 .
    RefSeqi WP_012330967.1. NC_010511.1.
    YP_001767984.1. NC_010511.1.

    3D structure databases

    ProteinModelPortali B0UCB6.
    SMRi B0UCB6. Positions 40-827.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 426117.M446_1019.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACA15550 ; ACA15550 ; M446_1019 .
    GeneIDi 6129780.
    KEGGi met:M446_1019.
    PATRICi 22583391. VBIMetSp32184_1062.

    Phylogenomic databases

    eggNOGi COG0243.
    HOGENOMi HOG000031441.
    KOi K02567.
    OMAi HQLVEAP.
    OrthoDBi EOG6CVV7G.

    Enzyme and pathway databases

    BioCyci MSP426117:GI2I-1027-MONOMER.

    Family and domain databases

    HAMAPi MF_01630. Nitrate_reduct.
    InterProi IPR009010. Asp_de-COase-like_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR027467. MopterinOxRdtase_cofactor_BS.
    IPR010051. Periplasm_NO3_reductase_lsu.
    IPR006311. TAT_signal.
    IPR019546. TAT_signal_bac_arc.
    [Graphical view ]
    Pfami PF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    [Graphical view ]
    SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50692. SSF50692. 1 hit.
    TIGRFAMsi TIGR01706. NAPA. 1 hit.
    TIGR01409. TAT_signal_seq. 1 hit.
    PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
    PS51318. TAT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 4-46.

    Entry informationi

    Entry nameiNAPA_METS4
    AccessioniPrimary (citable) accession number: B0UCB6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: April 8, 2008
    Last modified: October 1, 2014
    This is version 51 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3