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B0UCB6

- NAPA_METS4

UniProt

B0UCB6 - NAPA_METS4

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Protein
Periplasmic nitrate reductase
Gene
napA, M446_1019
Organism
Methylobacterium sp. (strain 4-46)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity.UniRule annotation

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster By similarity.UniRule annotation
Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi47 – 471Iron-sulfur (4Fe-4S) By similarity
Metal bindingi50 – 501Iron-sulfur (4Fe-4S) By similarity
Metal bindingi54 – 541Iron-sulfur (4Fe-4S) By similarity
Metal bindingi82 – 821Iron-sulfur (4Fe-4S) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. electron carrier activity Source: UniProtKB-HAMAP
  3. iron ion binding Source: UniProtKB-HAMAP
  4. molybdenum ion binding Source: InterPro
  5. nitrate reductase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
  2. nitrate assimilation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciMSP426117:GI2I-1027-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductase (EC:1.7.99.4)
Gene namesi
Name:napA
Ordered Locus Names:M446_1019
OrganismiMethylobacterium sp. (strain 4-46)
Taxonomic identifieri426117 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium
ProteomesiUP000001185: Chromosome

Subcellular locationi

Periplasm By similarity UniRule annotation

GO - Cellular componenti

  1. periplasmic space Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2929Tat-type signal Reviewed prediction
Add
BLAST
Chaini30 – 838809Periplasmic nitrate reductaseUniRule annotation
PRO_5000309931Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.UniRule annotation

Interactioni

Subunit structurei

Interacts with NapB By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi426117.M446_1019.

Structurei

3D structure databases

ProteinModelPortaliB0UCB6.
SMRiB0UCB6. Positions 40-827.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini40 – 96574Fe-4S Mo/W bis-MGD-type
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031441.
KOiK02567.
OMAiHQLVEAP.
OrthoDBiEOG6CVV7G.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B0UCB6-1 [UniParc]FASTAAdd to Basket

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MDMSRRTLLK AQAAAAAAAV AGIDLPAEAQ NLAAGEDIAL KWSKAPCRFC    50
GTGCGVMVGV KDGRVVATHG DMQAEVNRGL NCVKGYFLSK IMYGADRLTQ 100
PLMRVRGGKF DKNAELQPVS WDQAFDEMAR QWKRVLREKG PKAVGMFGSG 150
QWTIFEGYAA TKLMRAGFRS NNLDPNARHC MASAAVGFIR TFGMDEPMGC 200
YDDFEHADAF VLWGSNMAEM HPILWTRVID RRLSAPHVRI ATLSTYEHRT 250
TELSDQSLIF QPGTDLAILN FVANYIIQNG AVNRDFVERH VNFRVANTDI 300
GYGLRPEHVL EQRATHANEA AVSQPSNFDA YARMVGEYTL EKTEELTGVS 350
KERLLTLAKT YADPKIRVMS LWTMGFNQHV RGVWANHLVY NIHLLTGKIS 400
EPGNSPFSLT GQPSACGTAR EVGTFSHRLP ADMQVNNPEH RKHAEEIWKL 450
PPGLLNGEIG YHAVQQDRML KDGKLNAYWI MCNNNLQTAP NTNTETYPGY 500
RNPANFVVCS DPYPTVTAMA ADVVLPTAMW VEKEGAYGNA ERRTHMWRQL 550
VNAPGDARSD LWQLMEFSKR FTTDEVWAQE ILVAHPDYKG KSLFDVLWRN 600
GSVDRFGLDE IAADHENFES KQFGFYVQKG LFEEYASFGR GHGHDLAPFD 650
RYHEVRGLRW PVVDGKETRW RYREGHDPYV EKGAGWQFYG NPDRRAVVMA 700
APYEPPAEAP DQEYDLWLVT GRVLEHWHSG SMTMRVPELY RAFTGAVMFM 750
HPDDAQKRGL RRGQEVRIIS RRGEIRSRVE TRGRNKMPPG RVFVPFFDAS 800
QLINKATLDA TDPISKQTDF KKCAVKVIGV ATAERGAE 838
Length:838
Mass (Da):94,400
Last modified:April 8, 2008 - v1
Checksum:i11DFA43229E04111
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000943 Genomic DNA. Translation: ACA15550.1.
RefSeqiWP_012330967.1. NC_010511.1.
YP_001767984.1. NC_010511.1.

Genome annotation databases

EnsemblBacteriaiACA15550; ACA15550; M446_1019.
GeneIDi6129780.
KEGGimet:M446_1019.
PATRICi22583391. VBIMetSp32184_1062.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000943 Genomic DNA. Translation: ACA15550.1 .
RefSeqi WP_012330967.1. NC_010511.1.
YP_001767984.1. NC_010511.1.

3D structure databases

ProteinModelPortali B0UCB6.
SMRi B0UCB6. Positions 40-827.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 426117.M446_1019.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACA15550 ; ACA15550 ; M446_1019 .
GeneIDi 6129780.
KEGGi met:M446_1019.
PATRICi 22583391. VBIMetSp32184_1062.

Phylogenomic databases

eggNOGi COG0243.
HOGENOMi HOG000031441.
KOi K02567.
OMAi HQLVEAP.
OrthoDBi EOG6CVV7G.

Enzyme and pathway databases

BioCyci MSP426117:GI2I-1027-MONOMER.

Family and domain databases

HAMAPi MF_01630. Nitrate_reduct.
InterProi IPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view ]
Pfami PF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view ]
SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view ]
SUPFAMi SSF50692. SSF50692. 1 hit.
TIGRFAMsi TIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 4-46.

Entry informationi

Entry nameiNAPA_METS4
AccessioniPrimary (citable) accession number: B0UCB6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: April 8, 2008
Last modified: September 3, 2014
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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