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B0UCB6 (NAPA_METS4) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 49. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Periplasmic nitrate reductase

EC=1.7.99.4
Gene names
Name:napA
Ordered Locus Names:M446_1019
OrganismMethylobacterium sp. (strain 4-46) [Complete proteome] [HAMAP]
Taxonomic identifier426117 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium

Protein attributes

Sequence length838 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. HAMAP-Rule MF_01630

Catalytic activity

Nitrite + acceptor = nitrate + reduced acceptor. HAMAP-Rule MF_01630

Cofactor

Binds 1 4Fe-4S cluster By similarity. HAMAP-Rule MF_01630

Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Subunit structure

Interacts with NapB By similarity. HAMAP-Rule MF_01630

Subcellular location

Periplasm By similarity HAMAP-Rule MF_01630.

Post-translational modification

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP-Rule MF_01630

Sequence similarities

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.

Contains 1 4Fe-4S Mo/W bis-MGD-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2929Tat-type signal Potential
Chain30 – 838809Periplasmic nitrate reductase HAMAP-Rule MF_01630
PRO_5000309931

Regions

Domain40 – 96574Fe-4S Mo/W bis-MGD-type

Sites

Metal binding471Iron-sulfur (4Fe-4S) By similarity
Metal binding501Iron-sulfur (4Fe-4S) By similarity
Metal binding541Iron-sulfur (4Fe-4S) By similarity
Metal binding821Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
B0UCB6 [UniParc].

Last modified April 8, 2008. Version 1.
Checksum: 11DFA43229E04111

FASTA83894,400
        10         20         30         40         50         60 
MDMSRRTLLK AQAAAAAAAV AGIDLPAEAQ NLAAGEDIAL KWSKAPCRFC GTGCGVMVGV 

        70         80         90        100        110        120 
KDGRVVATHG DMQAEVNRGL NCVKGYFLSK IMYGADRLTQ PLMRVRGGKF DKNAELQPVS 

       130        140        150        160        170        180 
WDQAFDEMAR QWKRVLREKG PKAVGMFGSG QWTIFEGYAA TKLMRAGFRS NNLDPNARHC 

       190        200        210        220        230        240 
MASAAVGFIR TFGMDEPMGC YDDFEHADAF VLWGSNMAEM HPILWTRVID RRLSAPHVRI 

       250        260        270        280        290        300 
ATLSTYEHRT TELSDQSLIF QPGTDLAILN FVANYIIQNG AVNRDFVERH VNFRVANTDI 

       310        320        330        340        350        360 
GYGLRPEHVL EQRATHANEA AVSQPSNFDA YARMVGEYTL EKTEELTGVS KERLLTLAKT 

       370        380        390        400        410        420 
YADPKIRVMS LWTMGFNQHV RGVWANHLVY NIHLLTGKIS EPGNSPFSLT GQPSACGTAR 

       430        440        450        460        470        480 
EVGTFSHRLP ADMQVNNPEH RKHAEEIWKL PPGLLNGEIG YHAVQQDRML KDGKLNAYWI 

       490        500        510        520        530        540 
MCNNNLQTAP NTNTETYPGY RNPANFVVCS DPYPTVTAMA ADVVLPTAMW VEKEGAYGNA 

       550        560        570        580        590        600 
ERRTHMWRQL VNAPGDARSD LWQLMEFSKR FTTDEVWAQE ILVAHPDYKG KSLFDVLWRN 

       610        620        630        640        650        660 
GSVDRFGLDE IAADHENFES KQFGFYVQKG LFEEYASFGR GHGHDLAPFD RYHEVRGLRW 

       670        680        690        700        710        720 
PVVDGKETRW RYREGHDPYV EKGAGWQFYG NPDRRAVVMA APYEPPAEAP DQEYDLWLVT 

       730        740        750        760        770        780 
GRVLEHWHSG SMTMRVPELY RAFTGAVMFM HPDDAQKRGL RRGQEVRIIS RRGEIRSRVE 

       790        800        810        820        830 
TRGRNKMPPG RVFVPFFDAS QLINKATLDA TDPISKQTDF KKCAVKVIGV ATAERGAE 

« Hide

References

[1]"Complete sequence of chromosome of Methylobacterium sp. 4-46."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. expand/collapse author list , Kyrpides N., Ivanova N., Marx C.J., Richardson P.
Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 4-46.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000943 Genomic DNA. Translation: ACA15550.1.
RefSeqYP_001767984.1. NC_010511.1.

3D structure databases

ProteinModelPortalB0UCB6.
SMRB0UCB6. Positions 40-827.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING426117.M446_1019.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACA15550; ACA15550; M446_1019.
GeneID6129780.
KEGGmet:M446_1019.
PATRIC22583391. VBIMetSp32184_1062.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0243.
HOGENOMHOG000031441.
KOK02567.
OMAHQLVEAP.
OrthoDBEOG6CVV7G.

Enzyme and pathway databases

BioCycMSP426117:GI2I-1027-MONOMER.

Family and domain databases

HAMAPMF_01630. Nitrate_reduct.
InterProIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMSSF50692. SSF50692. 1 hit.
TIGRFAMsTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNAPA_METS4
AccessionPrimary (citable) accession number: B0UCB6
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: April 8, 2008
Last modified: May 14, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families