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Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Methylobacterium sp. (strain 4-46)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase complex NapAB. Receives electrons from NapB and catalyzes the reduction of nitrate to nitrite.UniRule annotation

Catalytic activityi

2 ferrocytochrome + nitrate + 2 H+ = 2 ferricytochrome + nitrite.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation
  • Mo-bis(molybdopterin guanine dinucleotide)UniRule annotationNote: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi47Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi50Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi54Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi82Iron-sulfur (4Fe-4S)UniRule annotation1
Binding sitei84Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei151Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei176Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei180Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei374Mo-bis(molybdopterin guanine dinucleotide); via amide nitrogenUniRule annotation1
Binding sitei378Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei484Mo-bis(molybdopterin guanine dinucleotide); via amide nitrogenUniRule annotation1
Binding sitei533Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei560Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei796Substrate; via amide nitrogenUniRule annotation1
Binding sitei804Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei821Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Nitrate assimilation, Transport
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductaseUniRule annotation (EC:1.9.6.1UniRule annotation)
Gene namesi
Name:napAUniRule annotation
Ordered Locus Names:M446_1019
OrganismiMethylobacterium sp. (strain 4-46)
Taxonomic identifieri426117 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium
Proteomesi
  • UP000001185 Componenti: Chromosome

Subcellular locationi

  • Periplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 29Tat-type signalUniRule annotationAdd BLAST29
ChainiPRO_500030993130 – 838Periplasmic nitrate reductaseUniRule annotationAdd BLAST809

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.UniRule annotation

Interactioni

Subunit structurei

Component of the periplasmic nitrate reductase NapAB complex composed of NapA and NapB.UniRule annotation

Protein-protein interaction databases

STRINGi426117.M446_1019

Structurei

3D structure databases

ProteinModelPortaliB0UCB6
SMRiB0UCB6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini40 – 964Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd BLAST57

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni213 – 220Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation8
Regioni244 – 248Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation5
Regioni263 – 265Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation3
Regioni510 – 511Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation2
Regioni720 – 729Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation10

Sequence similaritiesi

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4107QIW Bacteria
COG0243 LUCA
HOGENOMiHOG000031441
KOiK02567
OMAiTQHWRQQ
OrthoDBiPOG091H060P

Family and domain databases

HAMAPiMF_01630 Nitrate_reduct_NapA, 1 hit
InterProiView protein in InterPro
IPR009010 Asp_de-COase-like_dom_sf
IPR006657 MoPterin_dinucl-bd_dom
IPR006656 Mopterin_OxRdtase
IPR006963 Mopterin_OxRdtase_4Fe-4S_dom
IPR027467 MopterinOxRdtase_cofactor_BS
IPR010051 Periplasm_NO3_reductase_lsu
IPR006311 TAT_signal
IPR019546 TAT_signal_bac_arc
PANTHERiPTHR11615:SF123 PTHR11615:SF123, 1 hit
PfamiView protein in Pfam
PF04879 Molybdop_Fe4S4, 1 hit
PF00384 Molybdopterin, 1 hit
PF01568 Molydop_binding, 1 hit
SMARTiView protein in SMART
SM00926 Molybdop_Fe4S4, 1 hit
SUPFAMiSSF50692 SSF50692, 1 hit
TIGRFAMsiTIGR01706 NAPA, 1 hit
TIGR01409 TAT_signal_seq, 1 hit
PROSITEiView protein in PROSITE
PS51669 4FE4S_MOW_BIS_MGD, 1 hit
PS00551 MOLYBDOPTERIN_PROK_1, 1 hit
PS51318 TAT, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B0UCB6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDMSRRTLLK AQAAAAAAAV AGIDLPAEAQ NLAAGEDIAL KWSKAPCRFC
60 70 80 90 100
GTGCGVMVGV KDGRVVATHG DMQAEVNRGL NCVKGYFLSK IMYGADRLTQ
110 120 130 140 150
PLMRVRGGKF DKNAELQPVS WDQAFDEMAR QWKRVLREKG PKAVGMFGSG
160 170 180 190 200
QWTIFEGYAA TKLMRAGFRS NNLDPNARHC MASAAVGFIR TFGMDEPMGC
210 220 230 240 250
YDDFEHADAF VLWGSNMAEM HPILWTRVID RRLSAPHVRI ATLSTYEHRT
260 270 280 290 300
TELSDQSLIF QPGTDLAILN FVANYIIQNG AVNRDFVERH VNFRVANTDI
310 320 330 340 350
GYGLRPEHVL EQRATHANEA AVSQPSNFDA YARMVGEYTL EKTEELTGVS
360 370 380 390 400
KERLLTLAKT YADPKIRVMS LWTMGFNQHV RGVWANHLVY NIHLLTGKIS
410 420 430 440 450
EPGNSPFSLT GQPSACGTAR EVGTFSHRLP ADMQVNNPEH RKHAEEIWKL
460 470 480 490 500
PPGLLNGEIG YHAVQQDRML KDGKLNAYWI MCNNNLQTAP NTNTETYPGY
510 520 530 540 550
RNPANFVVCS DPYPTVTAMA ADVVLPTAMW VEKEGAYGNA ERRTHMWRQL
560 570 580 590 600
VNAPGDARSD LWQLMEFSKR FTTDEVWAQE ILVAHPDYKG KSLFDVLWRN
610 620 630 640 650
GSVDRFGLDE IAADHENFES KQFGFYVQKG LFEEYASFGR GHGHDLAPFD
660 670 680 690 700
RYHEVRGLRW PVVDGKETRW RYREGHDPYV EKGAGWQFYG NPDRRAVVMA
710 720 730 740 750
APYEPPAEAP DQEYDLWLVT GRVLEHWHSG SMTMRVPELY RAFTGAVMFM
760 770 780 790 800
HPDDAQKRGL RRGQEVRIIS RRGEIRSRVE TRGRNKMPPG RVFVPFFDAS
810 820 830
QLINKATLDA TDPISKQTDF KKCAVKVIGV ATAERGAE
Length:838
Mass (Da):94,400
Last modified:April 8, 2008 - v1
Checksum:i11DFA43229E04111
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000943 Genomic DNA Translation: ACA15550.1
RefSeqiWP_012330967.1, NC_010511.1

Genome annotation databases

EnsemblBacteriaiACA15550; ACA15550; M446_1019
KEGGimet:M446_1019

Similar proteinsi

Entry informationi

Entry nameiNAPA_METS4
AccessioniPrimary (citable) accession number: B0UCB6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: April 8, 2008
Last modified: March 28, 2018
This is version 70 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome