B0UBI8 (DADA_METS4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
D-amino acid dehydrogenase small subunit
EC=1.4.99.1

Gene names

Name:	dadA
Ordered Locus Names:	M446_2122

Organism

Methylobacterium sp. (strain 4-46) [Complete proteome] [HAMAP]

Taxonomic identifier

426117 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Methylobacteriaceae › Methylobacterium

Protein attributes

Sequence length	421 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Oxidative deamination of D-amino acids By similarity. HAMAP MF_01202
Catalytic activity	A D-amino acid + H2O + acceptor = a 2-oxo acid + NH3 + reduced acceptor. HAMAP MF_01202
Cofactor	FAD By similarity. HAMAP MF_01202
Pathway	Amino-acid degradation; D-alanine degradation; NH(3) and pyruvate from D-alanine: step 1/1. HAMAP MF_01202
Subunit structure	Heterodimer of a small and a large subunit By similarity.
Sequence similarities	Belongs to the DadA oxidoreductase family.

Ontologies

Keywords
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	D-amino-acid dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 421	421	D-amino acid dehydrogenase small subunit HAMAP MF_01202		PRO_1000138658
Regions
	Nucleotide binding	3 – 17	15	FAD Potential

Sequences

	Sequence		Length	Mass (Da)
	B0UBI8 [UniParc]. Last modified April 8, 2008. Version 1. Checksum: 2209BC09E83C775F	FASTA	421	45,749
	10 20 30 40 50 60 MRVLVLGGGV VGVTSAYYLA RAGHEVTVLD RQPGAGLETS FANAGQVSPG YSAPWAAPGI 70 80 90 100 110 120 PLKAMKWLMM RHRPLVLWPQ LEPRLYGWLA RMLANCTQEA YQRNKGRMVR LAEFSRDALR 130 140 150 160 170 180 DLRGETGIAY DHREKGTLQL FRTRKQLDHV GDDTRVLDAY GVAYEVLDPE GCIAAEPALA 190 200 210 220 230 240 RVRDTFVGGL RLPGDETGDA HLFTQRLAAL CADRGVRFRF GVAVARLHHE AGRVTGVETA 250 260 270 280 290 300 TGEVLRAEAY VAAMGSYTPA LLRPLGLSLP VYPVKGYSLT LPVTDEGSAP VSTVMDETYK 310 320 330 340 350 360 VAITRLGDRI RVGGTAELAG FSQALRGPRR ATLERSLTDL FPAGGDLSRA TFWTGLRPMT 370 380 390 400 410 420 PDGTPIVGAT AYGNLYTNTG HGTLGWTMAC GSGRMLADLI TGRHPEIAHE DFAERRYRRA A

References

[1]

"Complete sequence of chromosome of Methylobacterium sp. 4-46." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Ivanova N., Marx C.J., Richardson P. Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 4-46.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000943 Genomic DNA. Translation: ACA16584.1.
RefSeq	YP_001769018.1. NC_010511.1.
3D structure databases
ProteinModelPortal	B0UBI8.
ModBase	Search...
Protein-protein interaction databases
STRING	B0UBI8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6130683.
GenomeReviews	Gene locus M446_2122 in contig CP000943_GR.
KEGG	met:M446_2122.
PATRIC	22585464. VBIMetSp32184_2094.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG729204.
OMA	LARMLAN.
ProtClustDB	CLSK2328131.
Enzyme and pathway databases
BioCyc	MSP409:M446_2122-MONOMER.
Family and domain databases
HAMAP	MF_01202. DadA. [Tree]
InterPro	IPR023080. D-aa_DH_ssu_DadA. IPR006076. FAD-dep_OxRdtase. [Graphical view]
KO	K00285.
Pfam	PF01266. DAO. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DADA_METS4

Accession

Primary (citable) accession number: B0UBI8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	April 8, 2008
Last modified:	January 25, 2012
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program