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B0UB90 (DDL_METS4) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:M446_0922
OrganismMethylobacterium sp. (strain 4-46) [Complete proteome] [HAMAP]
Taxonomic identifier426117 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium

Protein attributes

Sequence length382 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 382382D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_0000341133

Regions

Domain139 – 348210ATP-grasp
Nucleotide binding168 – 22356ATP By similarity

Sites

Metal binding3001Magnesium or manganese 1 By similarity
Metal binding3151Magnesium or manganese 1 By similarity
Metal binding3151Magnesium or manganese 2 By similarity
Metal binding3171Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
B0UB90 [UniParc].

Last modified April 8, 2008. Version 1.
Checksum: 067AB507F7C4C5B6

FASTA38239,694
        10         20         30         40         50         60 
MRRNVALLFG GRSAEHEVSI VSAGNVARAL DPDRYAVTPI AIDKETGAWR LCPPLAPGEA 

        70         80         90        100        110        120 
PAMVREGPRV AFLPGGGGRL AVLGETASVS EPFDVVVPVL HGPNGEDGTV QGALDLAGVP 

       130        140        150        160        170        180 
YVGSGVIGSA AAMDKDVAKR LMRDAGLPIV PYLVAGPRRG VAYTEAVEAL ESRTLFVKPA 

       190        200        210        220        230        240 
NMGSSVGVSR VADAGQFDQA LAHAFAYDEK ILIERAVPRA REIEFAVLET AEGEVRVSPP 

       250        260        270        280        290        300 
GEIAPAAAHG FYGYDAKYVD PDGAALLIPA SLAPALAERM GGLAARAFEA LACAGLARVD 

       310        320        330        340        350        360 
LFLDPDDPEG IFVNEVNTLP GFTAISMYPK LWDAAGLAPP ALMDALIAHA LARHARAVAT 

       370        380 
GRAAPRSAAD AAWAPMRESI SR 

« Hide

References

[1]"Complete sequence of chromosome of Methylobacterium sp. 4-46."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. expand/collapse author list , Kyrpides N., Ivanova N., Marx C.J., Richardson P.
Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 4-46.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000943 Genomic DNA. Translation: ACA15471.1.
RefSeqYP_001767905.1. NC_010511.1.

3D structure databases

ProteinModelPortalB0UB90.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING426117.M446_0922.

Proteomic databases

PRIDEB0UB90.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACA15471; ACA15471; M446_0922.
GeneID6132029.
KEGGmet:M446_0922.
PATRIC22583233. VBIMetSp32184_0984.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011593.
KOK01921.
OMAPHELPRI.
OrthoDBEOG64BQ73.

Enzyme and pathway databases

BioCycMSP426117:GI2I-928-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_METS4
AccessionPrimary (citable) accession number: B0UB90
Entry history
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: April 8, 2008
Last modified: May 14, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways