B0U8Y5 (CYSD_METS4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sulfate adenylyltransferase subunit 2
EC=2.7.7.4

Alternative name(s):
ATP-sulfurylase small subunit
Sulfate adenylate transferase
Short name=SAT

Gene names

Name:	cysD
Ordered Locus Names:	M446_5480

Organism

Methylobacterium sp. (strain 4-46) [Complete proteome] [HAMAP]

Taxonomic identifier

426117 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Methylobacteriaceae › Methylobacterium

Protein attributes

Sequence length	311 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + sulfate = diphosphate + adenylyl sulfate. HAMAP MF_00064
Pathway	Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 1/3. HAMAP MF_00064
Subunit structure	Heterodimer composed of CysD, the smaller subunit, and CysN By similarity.
Sequence similarities	Belongs to the PAPS reductase family. CysD subfamily.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	sulfate reduction Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW sulfate adenylyltransferase (ATP) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 311	311	Sulfate adenylyltransferase subunit 2 HAMAP MF_00064		PRO_0000340204

Sequences

	Sequence		Length	Mass (Da)
	B0U8Y5 [UniParc]. Last modified April 8, 2008. Version 1. Checksum: 3DE4F2EC8097A31D	FASTA	311	35,557
	10 20 30 40 50 60 MSVALRSFAE TSPRLTHLQR LEAESIHIMR ETVAETENPV MLYSIGKDSS VLLHLALKAF 70 80 90 100 110 120 APGRLPFPLL HIDTTWKFRE MIAFRDRRAK ELGLDLLVYT NPDGLARGIG PVSHGSEVHT 130 140 150 160 170 180 DVMKTQALRQ ALDKHRFDAA FGGARRDEEA SRAKERVVSL RTAQHRWDPK RQRAEPWHLY 190 200 210 220 230 240 NLKKRRGESL RVFPLSNWTE LDIWLYIERE NIPIVPLYYA AERPVVRREG QLIMVDDARL 250 260 270 280 290 300 PLEPGETPEL RKVRFRTLGC YPLTGAVESE AATLPEIIGE TLAARTSERQ GRVIDKDGAG 310 AMERKKQEGY F

References

[1]

"Complete sequence of chromosome of Methylobacterium sp. 4-46." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Ivanova N., Marx C.J., Richardson P. Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 4-46.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000943 Genomic DNA. Translation: ACA19796.1.
RefSeq	YP_001772230.1. NC_010511.1.
3D structure databases
ProteinModelPortal	B0U8Y5.
SMR	B0U8Y5. Positions 14-221.
ModBase	Search...
Protein-protein interaction databases
STRING	B0U8Y5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6129637.
GenomeReviews	Gene locus M446_5480 in contig CP000943_GR.
KEGG	met:M446_5480.
PATRIC	22592192. VBIMetSp32184_5407.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG758022.
OMA	NARIHPG.
ProtClustDB	PRK05253.
Enzyme and pathway databases
BioCyc	MSP409:M446_5480-MONOMER.
Family and domain databases
HAMAP	MF_00064. Sulf_adenylyltr_sub2. [Tree]
InterPro	IPR002500. PAPS_reduct. IPR014729. Rossmann-like_a/b/a_fold. IPR011784. SO4_adenylTrfase_ssu. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KO	K00957.
Pfam	PF01507. PAPS_reduct. 1 hit. [Graphical view]
PIRSF	PIRSF002936. CysDAde_trans. 1 hit.
TIGRFAMs	TIGR02039. CysD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CYSD_METS4

Accession

Primary (citable) accession number: B0U8Y5

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	April 8, 2008
Last modified:	January 25, 2012
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program