true2009-07-282024-03-2784BIOB_METS4Complete sequence of chromosome of Methylobacterium sp. 4-46.Copeland A.Lucas S.Lapidus A.Glavina del Rio T.Dalin E.Tice H.Bruce D.Goodwin L.Pitluck S.Chertkov O.Brettin T.Detter J.C.Han C.Kuske C.R.Schmutz J.Larimer F.Land M.Hauser L.Kyrpides N.Ivanova N.Marx C.J.Richardson P.US DOE Joint Genome Institute2008-02EMBL/GenBank/DDBJNUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]4-46BacteriaRadical_SAMAldolase class IBioBAldolase_TIMBATS_domBiotin_synth/BioABBiotin_synthaseElp3/MiaA/NifB-like_rSAMrSAMbioBBIOTIN SYNTHASEBIOTIN SYNTHASE, MITOCHONDRIALBATSRadical_SAMBiotin_synthbiotin_synthasebiotin_synthase_likeBATSElp3Radical SAM enzymesRADICAL_SAMBiotin synthase2.8.1.6bioBM446_5411Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.Homodimer.Belongs to the radical SAM superfamily. Biotin synthase family.Biotin synthase354461332Radical SAM core462756165681061381982704Fe-4S-S-AdoMet2008-04-08135446ee952ade7ee5f44d0dab4711c6137648MTTTERSDPAIRHDWTVAQVQAIHDMPLLDLVHRASLVHRAHHDPSDIQRASLLSIKTGGCPEDCGYCSQSAHHKETGVARQRLMPVEAVLREAAAAKAAGATRFCMGAAWRSPKDGPDFDAVLAMVRGVRGLGMEACVTLGMLTPSQAERLAEAGLTAYNHNLDTGPDYYDKIVSTRSYEDRLATLQAVRDAGIGVCCGGIIGMGEGVTDRVAMLQVLANHAPHPESVPINALAAVPGTPLGERPPVDPFEMVRMCATARIVMPRARVRLSAGRRALSREAQVLCFLAGANSIFYGERLLTTANTDADADAQLLRDIGVPVPAISALEAAEtruetrue