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Protein

Pimeloyl-[acyl-carrier protein] methyl ester esterase

Gene

bioH

Organism
Xylella fastidiosa (strain M12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

The physiological role of BioH is to remove the methyl group introduced by BioC when the pimeloyl moiety is complete. It allows to synthesize pimeloyl-ACP via the fatty acid synthetic pathway through the hydrolysis of the ester bonds of pimeloyl-ACP esters.UniRule annotation

Catalytic activityi

Pimeloyl-[acyl-carrier protein] methyl ester + H2O = pimeloyl-[acyl-carrier protein] + methanol.UniRule annotation

Pathwayi: biotin biosynthesis

This protein is involved in the pathway biotin biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway biotin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei18Substrate; via amide nitrogen and carbonyl oxygenUniRule annotation1
Active sitei78NucleophileUniRule annotation1
Active sitei203UniRule annotation1
Active sitei233UniRule annotation1
Binding sitei233SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Serine esterase

Keywords - Biological processi

Biotin biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00078.

Protein family/group databases

ESTHERixylfa-XF1356. BioH.

Names & Taxonomyi

Protein namesi
Recommended name:
Pimeloyl-[acyl-carrier protein] methyl ester esteraseUniRule annotation (EC:3.1.1.85UniRule annotation)
Alternative name(s):
Biotin synthesis protein BioHUniRule annotation
Carboxylesterase BioHUniRule annotation
Gene namesi
Name:bioHUniRule annotation
Ordered Locus Names:Xfasm12_0709
OrganismiXylella fastidiosa (strain M12)
Taxonomic identifieri405440 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeXylella

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001400111 – 255Pimeloyl-[acyl-carrier protein] methyl ester esteraseAdd BLAST255

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB0U6I9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni78 – 79Substrate bindingUniRule annotation2
Regioni139 – 143Substrate bindingUniRule annotation5

Sequence similaritiesi

Belongs to the AB hydrolase superfamily. Carboxylesterase BioH family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000028062.
KOiK02170.
OMAiTIGEGDC.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
HAMAPiMF_01260. Carboxylester. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR010076. BioH.
[Graphical view]
PfamiPF12697. Abhydrolase_6. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
TIGRFAMsiTIGR01738. bioH. 1 hit.

Sequencei

Sequence statusi: Complete.

B0U6I9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYIEVTGYGP ALVLIHGWAM HSGVFAPLVE QLRPHHTLYL VDLPGHGYNH
60 70 80 90 100
TTPTPLALPQ VVHAIAAATP PAVWLGWSLG GLFALHAAAT LPQVRGLIML
110 120 130 140 150
AATPCFVRRE DWPHAVEVSI FTQFAQDLKQ NYTETINRFL ALDTLGSTHA
160 170 180 190 200
QSELRQLRKI LNARHTPNTA TLQAGLELLA HTDLRRAVID LTPPSLWIAG
210 220 230 240 250
QRDRLVPAAS IQAATALAPS GQTELLTITG GGHAPFLSHA NQMTAALQHF

IATLP
Length:255
Mass (Da):27,592
Last modified:April 8, 2008 - v1
Checksum:iB438D33CC65C82FF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000941 Genomic DNA. Translation: ACA11705.1.
RefSeqiWP_004083854.1. NC_010513.1.

Genome annotation databases

EnsemblBacteriaiACA11705; ACA11705; Xfasm12_0709.
KEGGixfm:Xfasm12_0709.
PATRICi24137784. VBIXylFas124301_0849.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000941 Genomic DNA. Translation: ACA11705.1.
RefSeqiWP_004083854.1. NC_010513.1.

3D structure databases

ProteinModelPortaliB0U6I9.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

ESTHERixylfa-XF1356. BioH.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACA11705; ACA11705; Xfasm12_0709.
KEGGixfm:Xfasm12_0709.
PATRICi24137784. VBIXylFas124301_0849.

Phylogenomic databases

HOGENOMiHOG000028062.
KOiK02170.
OMAiTIGEGDC.

Enzyme and pathway databases

UniPathwayiUPA00078.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
HAMAPiMF_01260. Carboxylester. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR010076. BioH.
[Graphical view]
PfamiPF12697. Abhydrolase_6. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
TIGRFAMsiTIGR01738. bioH. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiBIOH_XYLFM
AccessioniPrimary (citable) accession number: B0U6I9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: April 8, 2008
Last modified: November 2, 2016
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.