B0U5Q1 (MDH_XYLFM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Malate dehydrogenase
EC=1.1.1.37

Gene names

Name:	mdh
Ordered Locus Names:	Xfasm12_0550

Organism

Xylella fastidiosa (strain M12) [Complete proteome] [HAMAP]

Taxonomic identifier

405440 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Xanthomonadales › Xanthomonadaceae › Xylella › Xylella fastidiosa

Protein attributes

Sequence length	328 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible oxidation of malate to oxaloacetate By similarity. HAMAP-Rule MF_01517
Catalytic activity	(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP-Rule MF_01517
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 2 family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cellular carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro malate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Molecular_function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 328	328	Malate dehydrogenase HAMAP-Rule MF_01517		PRO_1000191634
Regions
	Nucleotide binding	11 – 17	7	NAD By similarity
	Nucleotide binding	131 – 133	3	NAD By similarity
Sites
	Active site	189	1	Proton acceptor By similarity
	Binding site	94	1	Substrate By similarity
	Binding site	100	1	Substrate By similarity
	Binding site	107	1	NAD By similarity
	Binding site	114	1	NAD By similarity
	Binding site	133	1	Substrate By similarity
	Binding site	164	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	B0U5Q1 [UniParc]. Last modified April 8, 2008. Version 1. Checksum: 3A9E3F2502D7B881	FASTA	328	35,448
	10 20 30 40 50 60 MKALVRVAVT GAAGQIGYSL LFRIAAGEMF GKDRSVILQM LELPDEKAQA ALKGVMMELE 70 80 90 100 110 120 DCAFPLLAGM VVTDNPDIAF KDADAALLVG SRPRGPGMER KDLLMENAKI FTAQGAALNK 130 140 150 160 170 180 VARRDVKVLV VGNPANTNAY IAMKSAPDLN PKHFTAMLRL DHNRALSQLS TKLSKPVANI 190 200 210 220 230 240 EKLIVWGNHS PTMYPDYRFA TADGTPIIEA INDQAWNANS FIPTVSKRGA AIIEARGLSS 250 260 270 280 290 300 AASAANAAID HMRDWLLGSN GKWITMGVPS DGSYGIPEGM IFGFPVTTTN GEYSIVKDLP 310 320 IDTFSKTYID KTLAELEEER ASIAHLLR

References

[1]

"Whole genome sequences of two Xylella fastidiosa strains (M12 and M23) causing almond leaf scorch disease in California." Chen J., Xie G., Han S., Chertkov O., Sims D., Civerolo E.L. J. Bacteriol. 192:4534-4534(2010) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: M12.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000941 Genomic DNA. Translation: ACA11555.1.
RefSeq	YP_001775185.1. NC_010513.1.
3D structure databases
ProteinModelPortal	B0U5Q1.
SMR	B0U5Q1. Positions 6-326.
ModBase	Search...
Protein-protein interaction databases
STRING	405440.Xfasm12_0550.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACA11555; ACA11555; Xfasm12_0550.
GeneID	6120957.
KEGG	xfm:Xfasm12_0550.
PATRIC	24137402. VBIXylFas124301_0658.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0039.
HOGENOM	HOG000220953.
KO	K00024.
OMA	NCLIASK.
ProtClustDB	PRK05442.
Family and domain databases
Gene3D	3.40.50.720. 1 hit. 3.90.110.10. 1 hit.
HAMAP	MF_01517. Malate_dehydrog_2.
InterPro	IPR001557. L-lactate/malate_DH. IPR022383. Lactate/malate_DH_C. IPR001236. Lactate/malate_DH_N. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR010945. Malate_DH_type2. IPR016040. NAD(P)-bd_dom. [Graphical view]
PANTHER	PTHR23382. PTHR23382. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
SUPFAM	SSF56327. Lactate_DH/Glyco_hydro_4_C. 1 hit.
TIGRFAMs	TIGR01759. MalateDH-SF1. 1 hit.
PROSITE	PS00068. MDH. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MDH_XYLFM

Accession

Primary (citable) accession number: B0U5Q1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	April 8, 2008
Last modified:	May 1, 2013
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program