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B0U3Q9

- SYI_XYLFM

UniProt

B0U3Q9 - SYI_XYLFM

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Protein
Isoleucine--tRNA ligase
Gene
ileS, Xfasm12_1578
Organism
Xylella fastidiosa (strain M12)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei577 – 5771Aminoacyl-adenylate By similarity
Binding sitei621 – 6211ATP By similarity
Metal bindingi906 – 9061Zinc By similarity
Metal bindingi909 – 9091Zinc By similarity
Metal bindingi926 – 9261Zinc By similarity
Metal bindingi929 – 9291Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciXFAS405440:GH0D-1613-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:Xfasm12_1578
OrganismiXylella fastidiosa (strain M12)
Taxonomic identifieri405440 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeXylella
ProteomesiUP000008539: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 943943Isoleucine--tRNA ligaseUniRule annotation
PRO_1000189218Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi405440.Xfasm12_1578.

Structurei

3D structure databases

ProteinModelPortaliB0U3Q9.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi59 – 6911"HIGH" regionUniRule annotation
Add
BLAST
Motifi618 – 6225"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B0U3Q9-1 [UniParc]FASTAAdd to Basket

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MSQDYKTTLH LPATDFPMRG DLPKREPAIL ERWERDDFYA QLRAHAKGRP    50
LFLLHDGPPY ANGQIHLGHA VNKILKDIII KSKHLDGFDA PYIPGWDCHG 100
LPIEIAIEKK YGKVGVTLDA VQFRQKCREY AAEQIQLQRR DFKRLGVIGD 150
WDAPYKTLDF RFEADEIRAL AKIVDKGHLI RGTKPVHWCF DCGSALAEAE 200
IEYTDKTSPM VDVAYPALDP SALAAVFNAT LPPDVQLAVP IWTTTPWTLP 250
ASLAISVGPT LDYVLVEGPT HSGQRRWLIL AEALAAKALA RYGIAELLIH 300
GSAKGAAMEQ HILAHPFYPD RTIPLLLGNH VSAEDGTGAV HTAPGHGQED 350
HQVFQQYGLL NRYSAAELNP VDARGVYLST TPPLGELTLA GLHIWKANPL 400
IVDALRLRGV LLAAAEMHHS YPHCWRHKTP IVFRATPQWF ISMEQAALRS 450
AALKAITHVT WYPQWGQARI LSMIENRPDW TISRQRTWGV PIPLFVHRHS 500
GAPHPRSAAL MRQIADRVEQ QGVDIWYSLD QTELLGTEAD QYEKITDILD 550
VWFDSGITHE AVLLERGLPK PADLYLEGAD QHRGWFQSSL LTGVAMDNAA 600
PYKQCLTHGF TVDQHGRKMS KSLGNGIEPQ DIIKTLGADI LRLWIASTDY 650
SNEMSLSQEI LKRTTDAYRR IRNTTRFLLG NLHGFDPTLH LVPLSDMIAL 700
DRWIVHRAFE LQQTIKAAYT RYDFAEIIQK ILNFCSVDLG SLYLDVTKDR 750
LYTMHENAPG RRSAQTAMYH LTAAFVRWIA PILSFTADEL WSYLPGDHAD 800
NVLFTTWYDG LAPLPPNAPL TAADFDKLLT LRDHVTKVLE PMRANGVIGA 850
ALEAEITVAA DADTAARWQP LTEELRFLFI TGDVTVTPAN TDGFFVSAQA 900
TTKAKCARCW HYRADIGAHP THPELCGRCI TNVDGPGEQR HWF 943
Length:943
Mass (Da):105,672
Last modified:April 8, 2008 - v1
Checksum:iA61A5E6210A0C42A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000941 Genomic DNA. Translation: ACA12488.1.
RefSeqiYP_001776118.1. NC_010513.1.

Genome annotation databases

EnsemblBacteriaiACA12488; ACA12488; Xfasm12_1578.
GeneIDi6119406.
KEGGixfm:Xfasm12_1578.
PATRICi24139794. VBIXylFas124301_1844.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000941 Genomic DNA. Translation: ACA12488.1 .
RefSeqi YP_001776118.1. NC_010513.1.

3D structure databases

ProteinModelPortali B0U3Q9.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 405440.Xfasm12_1578.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACA12488 ; ACA12488 ; Xfasm12_1578 .
GeneIDi 6119406.
KEGGi xfm:Xfasm12_1578.
PATRICi 24139794. VBIXylFas124301_1844.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci XFAS405440:GH0D-1613-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Whole genome sequences of two Xylella fastidiosa strains (M12 and M23) causing almond leaf scorch disease in California."
    Chen J., Xie G., Han S., Chertkov O., Sims D., Civerolo E.L.
    J. Bacteriol. 192:4534-4534(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: M12.

Entry informationi

Entry nameiSYI_XYLFM
AccessioniPrimary (citable) accession number: B0U3Q9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: April 8, 2008
Last modified: May 14, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi