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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Xylella fastidiosa (strain M12)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei577Aminoacyl-adenylateUniRule annotation1
Binding sitei621ATPUniRule annotation1
Metal bindingi906ZincUniRule annotation1
Metal bindingi909ZincUniRule annotation1
Metal bindingi926ZincUniRule annotation1
Metal bindingi929ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciXFAS405440:G1GB5-1576-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Xfasm12_1578
OrganismiXylella fastidiosa (strain M12)
Taxonomic identifieri405440 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeXylella

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001892181 – 943Isoleucine--tRNA ligaseAdd BLAST943

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB0U3Q9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi59 – 69"HIGH" regionAdd BLAST11
Motifi618 – 622"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000246402
KOiK01870
OMAiHLGTAWN

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
IPR010663 Znf_FPG/IleRS
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PF06827 zf-FPG_IleRS, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

B0U3Q9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQDYKTTLH LPATDFPMRG DLPKREPAIL ERWERDDFYA QLRAHAKGRP
60 70 80 90 100
LFLLHDGPPY ANGQIHLGHA VNKILKDIII KSKHLDGFDA PYIPGWDCHG
110 120 130 140 150
LPIEIAIEKK YGKVGVTLDA VQFRQKCREY AAEQIQLQRR DFKRLGVIGD
160 170 180 190 200
WDAPYKTLDF RFEADEIRAL AKIVDKGHLI RGTKPVHWCF DCGSALAEAE
210 220 230 240 250
IEYTDKTSPM VDVAYPALDP SALAAVFNAT LPPDVQLAVP IWTTTPWTLP
260 270 280 290 300
ASLAISVGPT LDYVLVEGPT HSGQRRWLIL AEALAAKALA RYGIAELLIH
310 320 330 340 350
GSAKGAAMEQ HILAHPFYPD RTIPLLLGNH VSAEDGTGAV HTAPGHGQED
360 370 380 390 400
HQVFQQYGLL NRYSAAELNP VDARGVYLST TPPLGELTLA GLHIWKANPL
410 420 430 440 450
IVDALRLRGV LLAAAEMHHS YPHCWRHKTP IVFRATPQWF ISMEQAALRS
460 470 480 490 500
AALKAITHVT WYPQWGQARI LSMIENRPDW TISRQRTWGV PIPLFVHRHS
510 520 530 540 550
GAPHPRSAAL MRQIADRVEQ QGVDIWYSLD QTELLGTEAD QYEKITDILD
560 570 580 590 600
VWFDSGITHE AVLLERGLPK PADLYLEGAD QHRGWFQSSL LTGVAMDNAA
610 620 630 640 650
PYKQCLTHGF TVDQHGRKMS KSLGNGIEPQ DIIKTLGADI LRLWIASTDY
660 670 680 690 700
SNEMSLSQEI LKRTTDAYRR IRNTTRFLLG NLHGFDPTLH LVPLSDMIAL
710 720 730 740 750
DRWIVHRAFE LQQTIKAAYT RYDFAEIIQK ILNFCSVDLG SLYLDVTKDR
760 770 780 790 800
LYTMHENAPG RRSAQTAMYH LTAAFVRWIA PILSFTADEL WSYLPGDHAD
810 820 830 840 850
NVLFTTWYDG LAPLPPNAPL TAADFDKLLT LRDHVTKVLE PMRANGVIGA
860 870 880 890 900
ALEAEITVAA DADTAARWQP LTEELRFLFI TGDVTVTPAN TDGFFVSAQA
910 920 930 940
TTKAKCARCW HYRADIGAHP THPELCGRCI TNVDGPGEQR HWF
Length:943
Mass (Da):105,672
Last modified:April 8, 2008 - v1
Checksum:iA61A5E6210A0C42A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000941 Genomic DNA Translation: ACA12488.1
RefSeqiWP_012337992.1, NC_010513.1

Genome annotation databases

EnsemblBacteriaiACA12488; ACA12488; Xfasm12_1578
KEGGixfm:Xfasm12_1578

Similar proteinsi

Entry informationi

Entry nameiSYI_XYLFM
AccessioniPrimary (citable) accession number: B0U3Q9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: April 8, 2008
Last modified: March 28, 2018
This is version 76 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health