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B0U3N1 (QUEF_XYLFM) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 44. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
NADPH-dependent 7-cyano-7-deazaguanine reductase
EC=1.7.1.13
Alternative name(s):
7-cyano-7-carbaguanine reductase
NADPH-dependent nitrile oxidoreductase
PreQ(0) reductase
Gene names
Name:queF
Ordered Locus Names:Xfasm12_1548
OrganismXylella fastidiosa (strain M12) [Complete proteome] [HAMAP]
Taxonomic identifier405440 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Xanthomonadales › Xanthomonadaceae › Xylella ›

Protein attributes

Sequence length275 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) By similarity. HAMAP-Rule MF_00817

Catalytic activity

7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH. HAMAP-Rule MF_00817

Pathway

tRNA modification; tRNA-queuosine biosynthesis. HAMAP-Rule MF_00817

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily.

Ontologies

Keywords
   Biological processQueuosine biosynthesis
   Cellular componentCytoplasm
   LigandNADP
   Molecular functionOxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processqueuosine biosynthetic process

Inferred from electronic annotation. Source: HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionoxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor

Inferred from electronic annotation. Source: HAMAP

preQ1 synthase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 275275NADPH-dependent 7-cyano-7-deazaguanine reductase HAMAP-Rule MF_00817
PRO_1000134287

Sequences

Sequence LengthMass (Da)Tools
B0U3N1 [UniParc].

Last modified April 8, 2008. Version 1.
Checksum: 63BFA44B495301B3

FASTA27530,780
        10         20         30         40         50         60 
MNTSHYSVLG HTVPYPKVYD PSLLFPISRA VGRTQIGIGV VLPFVGEDRW HAYELSWLDA 

        70         80         90        100        110        120 
RGKPCVATAT FHVPCDSPYL IESKSLKLYL NSFSAEVFNR AEALRLRIAA DLSACAGAAV 

       130        140        150        160        170        180 
AVEFGLPPVG GGDKEISLDR LNVDIEDYGP PNPDYLSNVA QNFVEEMVEE TLTSTLFKSN 

       190        200        210        220        230        240 
CPVTGQPDWA SVTVRYFGVP IDHEGLLRYF ISFRHHAEFH EQCVERIFQD VLQRCAPQCL 

       250        260        270 
AVEARYTRRG GLDINPLRTT SEMAWPLSVF RDPRQ

« Hide

References

[1]"Whole genome sequences of two Xylella fastidiosa strains (M12 and M23) causing almond leaf scorch disease in California."
Chen J., Xie G., Han S., Chertkov O., Sims D., Civerolo E.L.
J. Bacteriol. 192:4534-4534(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: M12.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000941 Genomic DNA. Translation: ACA12460.1.
RefSeqYP_001776090.1. NC_010513.1.

3D structure databases

ProteinModelPortalB0U3N1.
ModBaseSearch...

Protein-protein interaction databases

STRING405440.Xfasm12_1548.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACA12460; ACA12460; Xfasm12_1548.
GeneID6119989.
KEGGxfm:Xfasm12_1548.
PATRIC24139720. VBIXylFas124301_1808.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0780.
HOGENOMHOG000273755.
KOK06879.
OMAERIFCDL.
ProtClustDBPRK11792.

Enzyme and pathway databases

UniPathwayUPA00392.

Family and domain databases

HAMAPMF_00817. QueF_type2.
InterProIPR016428. CN_OxRdtase_NADPH-dep_YqcD.
IPR020602. GTP_CycHdrlase_I/CN_OxRdtase.
[Graphical view]
PfamPF01227. GTP_cyclohydroI. 1 hit.
[Graphical view]
PIRSFPIRSF004750. Nitrile_oxidored_YqcD_prd. 1 hit.
TIGRFAMsTIGR03138. QueF. 1 hit.
ProtoNetSearch...

Entry information

Entry nameQUEF_XYLFM
AccessionPrimary (citable) accession number: B0U3N1
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: April 8, 2008
Last modified: May 1, 2013
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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