B0U1I2 (DUT_XYLFM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Deoxyuridine 5'-triphosphate nucleotidohydrolase
Short name=dUTPase
EC=3.6.1.23

Alternative name(s):
dUTP pyrophosphatase

Gene names

Name:	dut
Ordered Locus Names:	Xfasm12_0127

Organism

Xylella fastidiosa (strain M12) [Complete proteome] [HAMAP]

Taxonomic identifier

405440 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Xanthomonadales › Xanthomonadaceae › Xylella › Xylella fastidiosa

Protein attributes

Sequence length	155 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA By similarity. HAMAP-Rule MF_00116
Catalytic activity	dUTP + H2O = dUMP + diphosphate. HAMAP-Rule MF_00116
Cofactor	Magnesium By similarity.
Pathway	Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2. HAMAP-Rule MF_00116
Sequence similarities	Belongs to the dUTPase family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	dUMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway dUTP metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	dUTP diphosphatase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 155	155	Deoxyuridine 5'-triphosphate nucleotidohydrolase HAMAP-Rule MF_00116		PRO_1000095004
Regions
	Region	74 – 76	3	Substrate binding By similarity
	Region	91 – 93	3	Substrate binding By similarity
Sites
	Binding site	87	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	B0U1I2 [UniParc]. Last modified April 8, 2008. Version 1. Checksum: 2E047246225348DF	FASTA	155	16,260
	10 20 30 40 50 60 MSAAVKPLQI KILDPRLGTV WPLPTYATEA SAGLDLRAAL DAPMTLVPGD AELLSTGIAI 70 80 90 100 110 120 HLVDPSLCAV VLPRSGLGHR HGIVLGNGTG LIDSDYQGPL LVSLWNRGRE AFTIEPGDRI 130 140 150 AQLVVLPIVR VVLQVVDTFV ESGRGAGGFG HTGVR

References

[1]

"Whole genome sequences of two Xylella fastidiosa strains (M12 and M23) causing almond leaf scorch disease in California." Chen J., Xie G., Han S., Chertkov O., Sims D., Civerolo E.L. J. Bacteriol. 192:4534-4534(2010) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: M12.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000941 Genomic DNA. Translation: ACA11166.1.
RefSeq	YP_001774796.1. NC_010513.1.
3D structure databases
ProteinModelPortal	B0U1I2.
SMR	B0U1I2. Positions 5-139.
ModBase	Search...
Protein-protein interaction databases
STRING	405440.Xfasm12_0127.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACA11166; ACA11166; Xfasm12_0127.
GeneID	6119838.
KEGG	xfm:Xfasm12_0127.
PATRIC	24136366. VBIXylFas124301_0158.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0756.
HOGENOM	HOG000028968.
KO	K01520.
OMA	ILPRSGM.
ProtClustDB	PRK00601.
Enzyme and pathway databases
UniPathway	UPA00610; UER00666.
Family and domain databases
HAMAP	MF_00116. dUTPase_bact.
InterPro	IPR008180. dUTP_pyroPase. IPR008181. dUTP_pyroPase_sf. [Graphical view]
PANTHER	PTHR11241. PTHR11241. 1 hit.
Pfam	PF00692. dUTPase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00576. dut. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DUT_XYLFM

Accession

Primary (citable) accession number: B0U1I2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	April 8, 2008
Last modified:	May 1, 2013
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program