B0U106 (SYE_FRAP2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS

Gene names

Name:	gltX
Ordered Locus Names:	Fphi_0603

Organism

Francisella philomiragia subsp. philomiragia (strain ATCC 25017) [Complete proteome] [HAMAP]

Taxonomic identifier

484022 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Thiotrichales › Francisellaceae › Francisella

Protein attributes

Sequence length	469 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 469	469	Glutamate--tRNA ligase HAMAP MF_00022_B		PRO_1000074321
Regions
	Motif	8 – 18	11	"HIGH" region HAMAP MF_00022_B
	Motif	236 – 240	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Metal binding	97	1	Zinc By similarity
	Metal binding	99	1	Zinc By similarity
	Metal binding	124	1	Zinc By similarity
	Metal binding	126	1	Zinc By similarity
	Binding site	239	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B0U106 [UniParc]. Last modified April 8, 2008. Version 1. Checksum: 282BDBDF2A417A66	FASTA	469	53,360
	10 20 30 40 50 60 MITTRFAPSP TGFLHVGGVR TALFSWLYAR HNNGKFLLRI EDTDLERSTQ AAVDAILDGM 70 80 90 100 110 120 SWLGLKNDEE IYYQTKRFDR YHEVIKQLIA EGKAYYCNCS KERLDELREH QQANNLKTGY 130 140 150 160 170 180 DGKCRDASYI PQEGDSFVVR FKNPQDGLVS WDDAVKGRIS ISNHELDDMI IQRTDGSPTY 190 200 210 220 230 240 NFCVVVDDID MAITHIIRGD DHVNNTPKQI NIYKALNAHV PVFAHVPMIL GPDGAKLSKR 250 260 270 280 290 300 HGAVNVMQYR EDGYLPQAIL NYLVRLGWSH GDQEIFSIDE MIKSFNLEHI NASPSRFDFD 310 320 330 340 350 360 KLKWLNKHYI KESKFEDIRT EVEYHFAKFG LDIANGPDLQ DLVAVMAEKV DTLVELTEKS 370 380 390 400 410 420 SYFYSDDIVY DEKAVKKHVK TTTGEIFIKL LENFEALETQ QWQDPDTLHN VVASTAEQCE 430 440 450 460 VGMGKVGMPL RIAITGSGQS PDIGITLKLL GKEKVLSRLQ RAIKELCNS

References

[1]

"Complete sequence of chromosome of Francisella philomiragia subsp. philomiragia ATCC 25017." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Richardson P. Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25017.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000937 Genomic DNA. Translation: ABZ86822.1.
RefSeq	YP_001677323.1. NC_010336.1.
3D structure databases
ProteinModelPortal	B0U106.
ModBase	Search...
Protein-protein interaction databases
STRING	B0U106.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5908728.
GenomeReviews	Gene locus Fphi_0603 in contig CP000937_GR.
KEGG	fph:Fphi_0603.
PATRIC	17938055. VBIFraPhi43880_0627.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG628189.
OMA	NATIIEM.
ProtClustDB	PRK01406.
Enzyme and pathway databases
BioCyc	FPHI484022:FPHI_0603-MONOMER.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE_FRAP2

Accession

Primary (citable) accession number: B0U106

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	April 8, 2008
Last modified:	January 25, 2012
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program