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Protein

Glutathione synthetase

Gene

gshB

Organism
Francisella philomiragia subsp. philomiragia (strain ATCC 25017)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.UniRule annotation

Cofactori

Mg2+UniRule annotation, Mn2+UniRule annotationNote: Binds 1 magnesium or manganese ion per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi281 – 2811Magnesium or manganeseUniRule annotation
Metal bindingi283 – 2831Magnesium or manganeseUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi151 – 20757ATPUniRule annotationAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. glutathione synthase activity Source: UniProtKB-HAMAP
  3. magnesium ion binding Source: UniProtKB-HAMAP
  4. manganese ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

LigaseUniRule annotationImported

Keywords - Biological processi

Glutathione biosynthesisUniRule annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotation, ManganeseUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciFPHI484022:GHVB-1867-MONOMER.
UniPathwayiUPA00142; UER00210.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione synthetaseUniRule annotation (EC:6.3.2.3UniRule annotation)
Alternative name(s):
GSH synthetaseUniRule annotation
Glutathione synthaseUniRule annotation
Gene namesi
Name:gshBUniRule annotation
Ordered Locus Names:Fphi_1813Imported
OrganismiFrancisella philomiragia subsp. philomiragia (strain ATCC 25017)Imported
Taxonomic identifieri484022 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaThiotrichalesFrancisellaceaeFrancisella
ProteomesiUP000001320 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: InterPro
Complete GO annotation...

Interactioni

Protein-protein interaction databases

STRINGi484022.Fphi_1813.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini125 – 310186ATP-graspUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the prokaryotic GSH synthase family.UniRule annotation
Contains 1 ATP-grasp domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG0189.
HOGENOMiHOG000265022.
KOiK01920.
OMAiMKPLHGH.
OrthoDBiEOG6WMHWB.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPiMF_00162. GSH_S.
InterProiIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR006284. Glut_synth_pro.
IPR004218. GSHS_ATP-bd.
IPR004215. GSHS_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PfamiPF02955. GSH-S_ATP. 1 hit.
PF02951. GSH-S_N. 1 hit.
[Graphical view]
SUPFAMiSSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01380. glut_syn. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B0U0T7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVGFIIDNL NSFNISKDST YMMLQAAQDK GWEIYTFYLN DLSIINGKPK
60 70 80 90 100
GDALKIKIHK AKESWYEILS QHHDLSLLDL DCIFMRKDPP FNMEYIYVTY
110 120 130 140 150
MLDLAKKHGV LIINNPQALR DFNEKVAISN YPKFAPNTLI TRSYKQINQF
160 170 180 190 200
YAEHKDIIVK PLDGMGGSSI FRIKDGDKNK NVILETLTHH ETRYIMVQDY
210 220 230 240 250
QEAIKDGDKR ILIVNGEPIK YLLARVPSDS DNRGNLAAGA RAEVRELQEQ
260 270 280 290 300
DYKIAKKVAK KLKKEGVMFA GLDVIGDKLT EVNITSPTGI QEIYKATKVN
310 320
AASLLMQAVE KKINKMRQEQ EHGE
Length:324
Mass (Da):37,048
Last modified:April 8, 2008 - v1
Checksum:i6EA984E0A431C408
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000937 Genomic DNA. Translation: ABZ88040.1.
RefSeqiWP_012281090.1. NC_010336.1.
YP_001678541.1. NC_010336.1.

Genome annotation databases

EnsemblBacteriaiABZ88040; ABZ88040; Fphi_1813.
KEGGifph:Fphi_1813.
PATRICi17940619. VBIFraPhi43880_1877.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000937 Genomic DNA. Translation: ABZ88040.1.
RefSeqiWP_012281090.1. NC_010336.1.
YP_001678541.1. NC_010336.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi484022.Fphi_1813.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABZ88040; ABZ88040; Fphi_1813.
KEGGifph:Fphi_1813.
PATRICi17940619. VBIFraPhi43880_1877.

Phylogenomic databases

eggNOGiCOG0189.
HOGENOMiHOG000265022.
KOiK01920.
OMAiMKPLHGH.
OrthoDBiEOG6WMHWB.

Enzyme and pathway databases

UniPathwayiUPA00142; UER00210.
BioCyciFPHI484022:GHVB-1867-MONOMER.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPiMF_00162. GSH_S.
InterProiIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR006284. Glut_synth_pro.
IPR004218. GSHS_ATP-bd.
IPR004215. GSHS_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PfamiPF02955. GSH-S_ATP. 1 hit.
PF02951. GSH-S_N. 1 hit.
[Graphical view]
SUPFAMiSSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01380. glut_syn. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of chromosome of Francisella philomiragia subsp. philomiragia ATCC 25017."
    US DOE Joint Genome Institute
    Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Richardson P.
    Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 25017Imported.

Entry informationi

Entry nameiB0U0T7_FRAP2
AccessioniPrimary (citable) accession number: B0U0T7
Entry historyi
Integrated into UniProtKB/TrEMBL: April 8, 2008
Last sequence update: April 8, 2008
Last modified: April 1, 2015
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.