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B0U0E8 (SYL_FRAP2) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Fphi_1752
OrganismFrancisella philomiragia subsp. philomiragia (strain ATCC 25017) [Complete proteome] [HAMAP]
Taxonomic identifier484022 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaThiotrichalesFrancisellaceaeFrancisella

Protein attributes

Sequence length813 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 813813Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000074834

Regions

Motif41 – 5111"HIGH" region HAMAP-Rule MF_00049
Motif575 – 5795"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5781ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B0U0E8 [UniParc].

Last modified April 8, 2008. Version 1.
Checksum: 1BEAB571428237E2

FASTA81393,274
        10         20         30         40         50         60 
MSEYNFTQIE QQAQEYWREN NSFKAVEDKN KEKFYCLSML PYPSGTLHMG HVRNYTIGDV 

        70         80         90        100        110        120 
IARYQKMQGK NVLHPMGWDA FGLPAENAAI KHKKSPYEWT KSNIAYMRSQ LDSLGFSFDW 

       130        140        150        160        170        180 
SREVATCDES YYKWEQWFFI QLYKKGLAYR KNSVVNWDPV DQTVLANEQV VDGRGWRSGA 

       190        200        210        220        230        240 
LIEKKEIPQW FLKITDYADE LLKDINQLDG WPEAVKTMQT NWIGKSKGLT VKFKIQNSDK 

       250        260        270        280        290        300 
EIEVFTTRPD TLMGVSYLGI APEHPLALEE AKTNSQLKSF IDECKRISTM EADLATQEKK 

       310        320        330        340        350        360 
GFKTSIQAIH PISGETVNVW VANFVLMGYG SGAVMSVPAH DQRDWEFAQK YNIALKQVIK 

       370        380        390        400        410        420 
PSDNKSKLDL DKEAFTEKGI LINSGEFDGL NFKSAYQAIK KYLFDNDKGY ETTNFRIHDW 

       430        440        450        460        470        480 
GISRQRYWGC PIPMIHCNDC GLVPEKEENL PVKLPTNVTL TEAGSPLKDI PEFLNVACPN 

       490        500        510        520        530        540 
CGKPATRETD TFDTFFESSW YYARYTCPTA DKMLSEEANY WLPVDKYIGG IEHAIMHLLY 

       550        560        570        580        590        600 
ARFFHKLMRD QGLVTSDEPF KNLLTQGMVL KDGAKMSKSK GNTVDPQELI DKYGADTVRL 

       610        620        630        640        650        660 
FSMFAAPPEQ SLEWSDTGVD GANKFLRKVY NYAYTNKEIL AKNITIDVTK LSKNDKKARY 

       670        680        690        700        710        720 
EIYANLKQAI FDFDKSQFNT VVSACMKILN TLNNYDNLSD SVKLEGFSIL LRILSPFTPH 

       730        740        750        760        770        780 
ICHYLWQEIG LGEDILHTQF PTVDDIALEK DEFLLVVQIN GKVKVKLELD ASLTKEQVEQ 

       790        800        810 
EVLSDEQIKT FIKDKQIVKV IYVPQKLINI VVK 

« Hide

References

[1]"Complete sequence of chromosome of Francisella philomiragia subsp. philomiragia ATCC 25017."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Richardson P.
Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 25017.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000937 Genomic DNA. Translation: ABZ87978.1.
RefSeqYP_001678479.1. NC_010336.1.

3D structure databases

ProteinModelPortalB0U0E8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING484022.Fphi_1752.

Proteomic databases

PRIDEB0U0E8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABZ87978; ABZ87978; Fphi_1752.
GeneID5908117.
KEGGfph:Fphi_1752.
PATRIC17940495. VBIFraPhi43880_1815.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAMAFAGPP.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycFPHI484022:GHVB-1805-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_FRAP2
AccessionPrimary (citable) accession number: B0U0E8
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: April 8, 2008
Last modified: May 14, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries