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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Francisella philomiragia subsp. philomiragia (strain ATCC 25017)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei578 – 5781ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciFPHI484022:GHVB-1805-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:Fphi_1752
OrganismiFrancisella philomiragia subsp. philomiragia (strain ATCC 25017)
Taxonomic identifieri484022 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaThiotrichalesFrancisellaceaeFrancisella
ProteomesiUP000001320: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 813813Leucine--tRNA ligasePRO_1000074834Add
BLAST

Proteomic databases

PRIDEiB0U0E8.

Interactioni

Protein-protein interaction databases

STRINGi484022.Fphi_1752.

Structurei

3D structure databases

ProteinModelPortaliB0U0E8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi41 – 5111"HIGH" regionAdd
BLAST
Motifi575 – 5795"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B0U0E8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEYNFTQIE QQAQEYWREN NSFKAVEDKN KEKFYCLSML PYPSGTLHMG
60 70 80 90 100
HVRNYTIGDV IARYQKMQGK NVLHPMGWDA FGLPAENAAI KHKKSPYEWT
110 120 130 140 150
KSNIAYMRSQ LDSLGFSFDW SREVATCDES YYKWEQWFFI QLYKKGLAYR
160 170 180 190 200
KNSVVNWDPV DQTVLANEQV VDGRGWRSGA LIEKKEIPQW FLKITDYADE
210 220 230 240 250
LLKDINQLDG WPEAVKTMQT NWIGKSKGLT VKFKIQNSDK EIEVFTTRPD
260 270 280 290 300
TLMGVSYLGI APEHPLALEE AKTNSQLKSF IDECKRISTM EADLATQEKK
310 320 330 340 350
GFKTSIQAIH PISGETVNVW VANFVLMGYG SGAVMSVPAH DQRDWEFAQK
360 370 380 390 400
YNIALKQVIK PSDNKSKLDL DKEAFTEKGI LINSGEFDGL NFKSAYQAIK
410 420 430 440 450
KYLFDNDKGY ETTNFRIHDW GISRQRYWGC PIPMIHCNDC GLVPEKEENL
460 470 480 490 500
PVKLPTNVTL TEAGSPLKDI PEFLNVACPN CGKPATRETD TFDTFFESSW
510 520 530 540 550
YYARYTCPTA DKMLSEEANY WLPVDKYIGG IEHAIMHLLY ARFFHKLMRD
560 570 580 590 600
QGLVTSDEPF KNLLTQGMVL KDGAKMSKSK GNTVDPQELI DKYGADTVRL
610 620 630 640 650
FSMFAAPPEQ SLEWSDTGVD GANKFLRKVY NYAYTNKEIL AKNITIDVTK
660 670 680 690 700
LSKNDKKARY EIYANLKQAI FDFDKSQFNT VVSACMKILN TLNNYDNLSD
710 720 730 740 750
SVKLEGFSIL LRILSPFTPH ICHYLWQEIG LGEDILHTQF PTVDDIALEK
760 770 780 790 800
DEFLLVVQIN GKVKVKLELD ASLTKEQVEQ EVLSDEQIKT FIKDKQIVKV
810
IYVPQKLINI VVK
Length:813
Mass (Da):93,274
Last modified:April 8, 2008 - v1
Checksum:i1BEAB571428237E2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000937 Genomic DNA. Translation: ABZ87978.1.
RefSeqiYP_001678479.1. NC_010336.1.

Genome annotation databases

EnsemblBacteriaiABZ87978; ABZ87978; Fphi_1752.
GeneIDi5908117.
KEGGifph:Fphi_1752.
PATRICi17940495. VBIFraPhi43880_1815.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000937 Genomic DNA. Translation: ABZ87978.1.
RefSeqiYP_001678479.1. NC_010336.1.

3D structure databases

ProteinModelPortaliB0U0E8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi484022.Fphi_1752.

Proteomic databases

PRIDEiB0U0E8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABZ87978; ABZ87978; Fphi_1752.
GeneIDi5908117.
KEGGifph:Fphi_1752.
PATRICi17940495. VBIFraPhi43880_1815.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciFPHI484022:GHVB-1805-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of chromosome of Francisella philomiragia subsp. philomiragia ATCC 25017."
    US DOE Joint Genome Institute
    Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Richardson P.
    Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 25017.

Entry informationi

Entry nameiSYL_FRAP2
AccessioniPrimary (citable) accession number: B0U0E8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: April 8, 2008
Last modified: January 7, 2015
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.