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B0TZQ5

- SYI_FRAP2

UniProt

B0TZQ5 - SYI_FRAP2

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Protein

Isoleucine--tRNA ligase

Gene
ileS, Fphi_0396
Organism
Francisella philomiragia subsp. philomiragia (strain ATCC 25017)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei558 – 5581Aminoacyl-adenylate By similarity
Binding sitei602 – 6021ATP By similarity
Metal bindingi897 – 8971Zinc By similarity
Metal bindingi900 – 9001Zinc By similarity
Metal bindingi917 – 9171Zinc By similarity
Metal bindingi920 – 9201Zinc By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciFPHI484022:GHVB-403-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:Fphi_0396
OrganismiFrancisella philomiragia subsp. philomiragia (strain ATCC 25017)
Taxonomic identifieri484022 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaThiotrichalesFrancisellaceaeFrancisella
ProteomesiUP000001320: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 935935Isoleucine--tRNA ligaseUniRule annotationPRO_1000088546Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi484022.Fphi_0396.

Structurei

3D structure databases

ProteinModelPortaliB0TZQ5.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionUniRule annotationAdd
BLAST
Motifi599 – 6035"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B0TZQ5-1 [UniParc]FASTAAdd to Basket

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MSDYKDTLNL PKTSFSMKGN LANKEPMILN KWEKQGIYKK IREHFAGREK    50
FVLHDGPPYA NGSIHVGHAV NKILKDIIVK SKTLSGYDAP YIPTWDCHGL 100
PIELQVEKKH GKAGQKISED AFRKECRKYA KQQVEIQKKD FKRLGVLGQW 150
DQPYLTMDFS YEANMIRTLA KIIENKHLTK GFKPVHWCTD CGSALAEAEV 200
EYKDKISPAI DVKFKIKDKE KLAKAFGLES LNHDAFAIIW TTTPWTLPAN 250
QAIAVNNQLN YSLVKIEDFY IILAENLVEQ TLKRYAIENA QVIANTSGYK 300
LIGIIAEHPF YSRHVPILHG DHVTDDSGTG MVHTAPTHGV EDFTLGKEHD 350
LSMEIFVKGN GCYSENTKLF AGEFVFKAND RVIELLGEKK RLMNFDKLKH 400
SYPHCWRHKT PLIFRATPQW FISMEKEGLR EKAIEAIKET SWAPSWGQAR 450
IEGMIKDRPD WCISRQRTWG VPLPLFIHKE TEELHPNTIE ILHKVAQKIE 500
KGGIEAWFNA DDNEFIAETD KYKRVKDTLD VWFDSGSSSM CILDIDKSLS 550
YPADLYLEGS DQHRGWFQTS LLVAMSAKGN QPYKEVFTHG FVVDEHGRKM 600
SKSLGNVTSP QDIYNTLGAD ILRLWTASTD YKSEMAVSDQ ILKRTADTYR 650
RLRNTARFLL SNLEGFNPET DIIEFDKLVK LDQWAIAKTK EFQDKIIEAY 700
DKYQTHTVAQ LIHHFCSIEM GSFYLDIIKD RQYTAKADGH PRKSAQTAIY 750
HIVHALVRWM APILSYTADE IWEATPKTTD LPIQLCEWYT DLKSFNDQDE 800
LNLEFWAKIQ EIRSEVNRIL EIKRNEEVIK ASLEAEIIIY ADNDNYKLLE 850
KLGNELRFLL ISSKASLRAI EEKTNNSIES NITGLNIEVN KIEEPKCERC 900
WHRSATVGQN EEYQDICSRC VENITTEAGE SREFA 935
Length:935
Mass (Da):107,504
Last modified:April 8, 2008 - v1
Checksum:iC82D382AC612D76C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000937 Genomic DNA. Translation: ABZ86614.1.
RefSeqiWP_012279887.1. NC_010336.1.
YP_001677115.1. NC_010336.1.

Genome annotation databases

EnsemblBacteriaiABZ86614; ABZ86614; Fphi_0396.
GeneIDi5907234.
KEGGifph:Fphi_0396.
PATRICi17937619. VBIFraPhi43880_0412.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000937 Genomic DNA. Translation: ABZ86614.1 .
RefSeqi WP_012279887.1. NC_010336.1.
YP_001677115.1. NC_010336.1.

3D structure databases

ProteinModelPortali B0TZQ5.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 484022.Fphi_0396.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABZ86614 ; ABZ86614 ; Fphi_0396 .
GeneIDi 5907234.
KEGGi fph:Fphi_0396.
PATRICi 17937619. VBIFraPhi43880_0412.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci FPHI484022:GHVB-403-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of chromosome of Francisella philomiragia subsp. philomiragia ATCC 25017."
    US DOE Joint Genome Institute
    Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Richardson P.
    Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 25017.

Entry informationi

Entry nameiSYI_FRAP2
AccessioniPrimary (citable) accession number: B0TZQ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: April 8, 2008
Last modified: September 3, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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