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B0TZQ5

- SYI_FRAP2

UniProt

B0TZQ5 - SYI_FRAP2

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Francisella philomiragia subsp. philomiragia (strain ATCC 25017)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei558 – 5581Aminoacyl-adenylateUniRule annotation
Binding sitei602 – 6021ATPUniRule annotation
Metal bindingi897 – 8971ZincUniRule annotation
Metal bindingi900 – 9001ZincUniRule annotation
Metal bindingi917 – 9171ZincUniRule annotation
Metal bindingi920 – 9201ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciFPHI484022:GHVB-403-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Fphi_0396
OrganismiFrancisella philomiragia subsp. philomiragia (strain ATCC 25017)
Taxonomic identifieri484022 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaThiotrichalesFrancisellaceaeFrancisella
ProteomesiUP000001320: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 935935Isoleucine--tRNA ligasePRO_1000088546Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi484022.Fphi_0396.

Structurei

3D structure databases

ProteinModelPortaliB0TZQ5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionAdd
BLAST
Motifi599 – 6035"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B0TZQ5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSDYKDTLNL PKTSFSMKGN LANKEPMILN KWEKQGIYKK IREHFAGREK
60 70 80 90 100
FVLHDGPPYA NGSIHVGHAV NKILKDIIVK SKTLSGYDAP YIPTWDCHGL
110 120 130 140 150
PIELQVEKKH GKAGQKISED AFRKECRKYA KQQVEIQKKD FKRLGVLGQW
160 170 180 190 200
DQPYLTMDFS YEANMIRTLA KIIENKHLTK GFKPVHWCTD CGSALAEAEV
210 220 230 240 250
EYKDKISPAI DVKFKIKDKE KLAKAFGLES LNHDAFAIIW TTTPWTLPAN
260 270 280 290 300
QAIAVNNQLN YSLVKIEDFY IILAENLVEQ TLKRYAIENA QVIANTSGYK
310 320 330 340 350
LIGIIAEHPF YSRHVPILHG DHVTDDSGTG MVHTAPTHGV EDFTLGKEHD
360 370 380 390 400
LSMEIFVKGN GCYSENTKLF AGEFVFKAND RVIELLGEKK RLMNFDKLKH
410 420 430 440 450
SYPHCWRHKT PLIFRATPQW FISMEKEGLR EKAIEAIKET SWAPSWGQAR
460 470 480 490 500
IEGMIKDRPD WCISRQRTWG VPLPLFIHKE TEELHPNTIE ILHKVAQKIE
510 520 530 540 550
KGGIEAWFNA DDNEFIAETD KYKRVKDTLD VWFDSGSSSM CILDIDKSLS
560 570 580 590 600
YPADLYLEGS DQHRGWFQTS LLVAMSAKGN QPYKEVFTHG FVVDEHGRKM
610 620 630 640 650
SKSLGNVTSP QDIYNTLGAD ILRLWTASTD YKSEMAVSDQ ILKRTADTYR
660 670 680 690 700
RLRNTARFLL SNLEGFNPET DIIEFDKLVK LDQWAIAKTK EFQDKIIEAY
710 720 730 740 750
DKYQTHTVAQ LIHHFCSIEM GSFYLDIIKD RQYTAKADGH PRKSAQTAIY
760 770 780 790 800
HIVHALVRWM APILSYTADE IWEATPKTTD LPIQLCEWYT DLKSFNDQDE
810 820 830 840 850
LNLEFWAKIQ EIRSEVNRIL EIKRNEEVIK ASLEAEIIIY ADNDNYKLLE
860 870 880 890 900
KLGNELRFLL ISSKASLRAI EEKTNNSIES NITGLNIEVN KIEEPKCERC
910 920 930
WHRSATVGQN EEYQDICSRC VENITTEAGE SREFA
Length:935
Mass (Da):107,504
Last modified:April 8, 2008 - v1
Checksum:iC82D382AC612D76C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000937 Genomic DNA. Translation: ABZ86614.1.
RefSeqiYP_001677115.1. NC_010336.1.

Genome annotation databases

EnsemblBacteriaiABZ86614; ABZ86614; Fphi_0396.
GeneIDi5907234.
KEGGifph:Fphi_0396.
PATRICi17937619. VBIFraPhi43880_0412.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000937 Genomic DNA. Translation: ABZ86614.1 .
RefSeqi YP_001677115.1. NC_010336.1.

3D structure databases

ProteinModelPortali B0TZQ5.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 484022.Fphi_0396.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABZ86614 ; ABZ86614 ; Fphi_0396 .
GeneIDi 5907234.
KEGGi fph:Fphi_0396.
PATRICi 17937619. VBIFraPhi43880_0412.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci FPHI484022:GHVB-403-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of chromosome of Francisella philomiragia subsp. philomiragia ATCC 25017."
    US DOE Joint Genome Institute
    Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Richardson P.
    Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 25017.

Entry informationi

Entry nameiSYI_FRAP2
AccessioniPrimary (citable) accession number: B0TZQ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: April 8, 2008
Last modified: November 26, 2014
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3