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B0TZI5 (GLGB_FRAP2) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 48. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan branching enzyme GlgB

EC=2.4.1.18
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE
Gene names
Name:glgB
Ordered Locus Names:Fphi_0328
OrganismFrancisella philomiragia subsp. philomiragia (strain ATCC 25017) [Complete proteome] [HAMAP]
Taxonomic identifier484022 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaThiotrichalesFrancisellaceaeFrancisella

Protein attributes

Sequence length640 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685

Pathway

Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00685

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 6406401,4-alpha-glucan branching enzyme GlgB HAMAP-Rule MF_00685
PRO_1000083067

Sites

Active site3181Nucleophile By similarity
Active site3711Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
B0TZI5 [UniParc].

Last modified April 8, 2008. Version 1.
Checksum: AA6BC602D643F232

FASTA64074,475
        10         20         30         40         50         60 
MKNVNSKQNN HSTIGEQDIH FFHEGKHIYA YEFMGAHKAS EDGIDGIRFT TWAPNAKSIC 

        70         80         90        100        110        120 
VIGDFNHWQV EDKNHMKRIT DAGLWSVFIA DVKDGDKYKF VVTNKDTNHY VYKSDPYAFF 

       130        140        150        160        170        180 
SELRPNTASV ISTQTTYKWH DEKWLKKRAT ADYYNSPMNT YELHLASWKT KDDRFMTYEE 

       190        200        210        220        230        240 
IAEVLPKYVK DMGYTHVEFM PLHEHPLDAS WGYQPTGFYS INSRHGDLVG LKHLVDKLHT 

       250        260        270        280        290        300 
HDIGVILDWV PGHFCKDQHG LINFDGSACY EYQEPTKAIN KGWGTHNFDL GRNEVKCFLI 

       310        320        330        340        350        360 
SNAMYWINEF HIDGLRVDAV SNILYLNYDR EDGQWVPNIH GGHENLEGIA FLRELNGVLK 

       370        380        390        400        410        420 
HTCKGVITIA EESSSWPNIS TPVEQGGLGF DFKWNMGWMN DTLRYISLDP VYRKYHHNLI 

       430        440        450        460        470        480 
TFSMVYHYSE KFILSISHDE VVHGKKSLIN KMWGDLWNKY AGLRLYMSFM IGHPGKKLIF 

       490        500        510        520        530        540 
MGSEFGQFIE WREYEQLQWQ VVDEYHTHRE TLNFFKKLNE FYKAETALWE CDYDHKGFQW 

       550        560        570        580        590        600 
IDANNSEQSI LSFVRSNKDG KEKLIFVCNF TPVTYYDYHI GVPDAGSYIE AFNSDDLEFG 

       610        620        630        640 
GSGQLIADEI FSTPESSHGF DQRITIKVPP MATLVLKLKK 

« Hide

References

[1]"Complete sequence of chromosome of Francisella philomiragia subsp. philomiragia ATCC 25017."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Richardson P.
Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 25017.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000937 Genomic DNA. Translation: ABZ86544.1.
RefSeqYP_001677045.1. NC_010336.1.

3D structure databases

ProteinModelPortalB0TZI5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING484022.Fphi_0328.

Protein family/group databases

CAZyCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABZ86544; ABZ86544; Fphi_0328.
GeneID5907381.
KEGGfph:Fphi_0328.
PATRIC17937455. VBIFraPhi43880_0337.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0296.
HOGENOMHOG000283037.
KOK00700.
OMAAKLLFMG.
OrthoDBEOG6JX7GT.

Enzyme and pathway databases

BioCycFPHI484022:GHVB-326-MONOMER.
UniPathwayUPA00164.

Family and domain databases

Gene3D2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_00685. GlgB.
InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
TIGRFAMsTIGR01515. branching_enzym. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLGB_FRAP2
AccessionPrimary (citable) accession number: B0TZI5
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: April 8, 2008
Last modified: June 11, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries