B0TZ54 (RSMA_FRAP2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA small subunit methyltransferase A
EC=2.1.1.182

Alternative name(s):
16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase
16S rRNA dimethyladenosine transferase
16S rRNA dimethylase
S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Gene names

Name:	rsmA
Synonyms:	ksgA
Ordered Locus Names:	Fphi_0277

Organism

Francisella philomiragia subsp. philomiragia (strain ATCC 25017) [Complete proteome] [HAMAP]

Taxonomic identifier

484022 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Thiotrichales › Francisellaceae › Francisella › Francisella philomiragia

Protein attributes

Sequence length	262 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits By similarity. HAMAP-Rule MF_00607
Catalytic activity	4 S-adenosyl-L-methionine + adenine(1518)/adenine(1519) in 16S rRNA = 4 S-adenosyl-L-homocysteine + N(6)-dimethyladenine(1518)/N(6)-dimethyladenine(1519) in 16S rRNA. HAMAP-Rule MF_00607
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_00607.
Sequence similarities	Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	RNA-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	rRNA methylation Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase activity Inferred from electronic annotation. Source: EC RNA binding Inferred from electronic annotation. Source: UniProtKB-KW rRNA (adenine-N6,N6-)-dimethyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 262	262	Ribosomal RNA small subunit methyltransferase A HAMAP-Rule MF_00607		PRO_1000082553
Sites
	Binding site	14	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	16	1	S-adenosyl-L-methionine; via amide nitrogen By similarity
	Binding site	41	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	62	1	S-adenosyl-L-methionine By similarity
	Binding site	87	1	S-adenosyl-L-methionine By similarity
	Binding site	109	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	B0TZ54 [UniParc]. Last modified April 8, 2008. Version 1. Checksum: AB53E14AE2B5090F	FASTA	262	30,131
	10 20 30 40 50 60 MQYKTKAKKS LGQNFLQDEN IIRKIVQLAN IKKDDIVLEI GPGLGALTRY ILKCSDNVNV 70 80 90 100 110 120 VEFDASVIDT LLQNCQKYGE PKVFNEDFLK FDLDIVRADS QQKLKLIGNL PYNISSPILF 130 140 150 160 170 180 KVIEQSDKII DAHFMLQKEV VERIISQPNS KTYGRLSVIL QYHFDCSMIL NIPPEVFYPQ 190 200 210 220 230 240 PKVDSAILRL KPKANKLVIN DYIFFENIVK QSFAQRRKTL HNNLKGILKE LDIDPNTLPV 250 260 DTKLRAENLS MEDFVSLVNF LK

References

[1]

"Complete sequence of chromosome of Francisella philomiragia subsp. philomiragia ATCC 25017." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Richardson P. Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25017.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000937 Genomic DNA. Translation: ABZ86493.1.
RefSeq	YP_001676994.1. NC_010336.1.
3D structure databases
ProteinModelPortal	B0TZ54.
ModBase	Search...
Protein-protein interaction databases
STRING	484022.Fphi_0277.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABZ86493; ABZ86493; Fphi_0277.
GeneID	5909013.
KEGG	fph:Fphi_0277.
PATRIC	17937353. VBIFraPhi43880_0286.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0030.
HOGENOM	HOG000227962.
KO	K02528.
OMA	PVPNVDS.
ProtClustDB	PRK00274.
Enzyme and pathway databases
BioCyc	FPHI484022:GHVB-275-MONOMER.
Family and domain databases
Gene3D	1.10.8.100. 1 hit.
HAMAP	MF_00607. 16SrRNA_methyltr_A.
InterPro	IPR023165. rRNA_Ade_diMease-like. IPR020596. rRNA_Ade_Mease_Trfase_CS. IPR001737. rRNA_Ade_methylase_transferase. IPR020598. rRNA_Ade_methylase_Trfase_N. IPR011530. rRNA_adenine_dimethylase. [Graphical view]
PANTHER	PTHR11727. PTHR11727. 1 hit.
Pfam	PF00398. RrnaAD. 1 hit. [Graphical view]
SMART	SM00650. rADc. 1 hit. [Graphical view]
TIGRFAMs	TIGR00755. ksgA. 1 hit.
PROSITE	PS01131. RRNA_A_DIMETH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RSMA_FRAP2

Accession

Primary (citable) accession number: B0TZ54

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	April 8, 2008
Last modified:	May 1, 2013
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program