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B0TZ54 (RSMA_FRAP2) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribosomal RNA small subunit methyltransferase A

EC=2.1.1.182
Alternative name(s):
16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase
16S rRNA dimethyladenosine transferase
16S rRNA dimethylase
S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase
Gene names
Name:rsmA
Synonyms:ksgA
Ordered Locus Names:Fphi_0277
OrganismFrancisella philomiragia subsp. philomiragia (strain ATCC 25017) [Complete proteome] [HAMAP]
Taxonomic identifier484022 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaThiotrichalesFrancisellaceaeFrancisella

Protein attributes

Sequence length262 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits By similarity. HAMAP-Rule MF_00607

Catalytic activity

4 S-adenosyl-L-methionine + adenine(1518)/adenine(1519) in 16S rRNA = 4 S-adenosyl-L-homocysteine + N(6)-dimethyladenine(1518)/N(6)-dimethyladenine(1519) in 16S rRNA. HAMAP-Rule MF_00607

Subcellular location

Cytoplasm Potential HAMAP-Rule MF_00607.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 262262Ribosomal RNA small subunit methyltransferase A HAMAP-Rule MF_00607
PRO_1000082553

Sites

Binding site141S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding site161S-adenosyl-L-methionine; via amide nitrogen By similarity
Binding site411S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding site621S-adenosyl-L-methionine By similarity
Binding site871S-adenosyl-L-methionine By similarity
Binding site1091S-adenosyl-L-methionine By similarity

Sequences

Sequence LengthMass (Da)Tools
B0TZ54 [UniParc].

Last modified April 8, 2008. Version 1.
Checksum: AB53E14AE2B5090F

FASTA26230,131
        10         20         30         40         50         60 
MQYKTKAKKS LGQNFLQDEN IIRKIVQLAN IKKDDIVLEI GPGLGALTRY ILKCSDNVNV 

        70         80         90        100        110        120 
VEFDASVIDT LLQNCQKYGE PKVFNEDFLK FDLDIVRADS QQKLKLIGNL PYNISSPILF 

       130        140        150        160        170        180 
KVIEQSDKII DAHFMLQKEV VERIISQPNS KTYGRLSVIL QYHFDCSMIL NIPPEVFYPQ 

       190        200        210        220        230        240 
PKVDSAILRL KPKANKLVIN DYIFFENIVK QSFAQRRKTL HNNLKGILKE LDIDPNTLPV 

       250        260 
DTKLRAENLS MEDFVSLVNF LK 

« Hide

References

[1]"Complete sequence of chromosome of Francisella philomiragia subsp. philomiragia ATCC 25017."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Richardson P.
Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 25017.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000937 Genomic DNA. Translation: ABZ86493.1.
RefSeqYP_001676994.1. NC_010336.1.

3D structure databases

ProteinModelPortalB0TZ54.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING484022.Fphi_0277.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABZ86493; ABZ86493; Fphi_0277.
GeneID5909013.
KEGGfph:Fphi_0277.
PATRIC17937353. VBIFraPhi43880_0286.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0030.
HOGENOMHOG000227962.
KOK02528.
OMAHIPRKRF.
OrthoDBEOG66F08Z.
ProtClustDBPRK00274.

Enzyme and pathway databases

BioCycFPHI484022:GHVB-275-MONOMER.

Family and domain databases

Gene3D1.10.8.100. 1 hit.
HAMAPMF_00607. 16SrRNA_methyltr_A.
InterProIPR023165. rRNA_Ade_diMease-like.
IPR020596. rRNA_Ade_Mease_Trfase_CS.
IPR001737. rRNA_Ade_methylase_transferase.
IPR020598. rRNA_Ade_methylase_Trfase_N.
IPR011530. rRNA_adenine_dimethylase.
[Graphical view]
PANTHERPTHR11727. PTHR11727. 1 hit.
PfamPF00398. RrnaAD. 1 hit.
[Graphical view]
SMARTSM00650. rADc. 1 hit.
[Graphical view]
TIGRFAMsTIGR00755. ksgA. 1 hit.
PROSITEPS01131. RRNA_A_DIMETH. 1 hit.
PS51689. SAM_RNA_A_N6_MT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRSMA_FRAP2
AccessionPrimary (citable) accession number: B0TZ54
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: April 8, 2008
Last modified: April 16, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families