Reviewed, UniProtKB/Swiss-Prot B0TYR8 (TDH_FRAP2)

Names and origin

Protein names

Recommended name:
    L-threonine 3-dehydrogenase
    EC=1.1.1.103

Gene names

Name:	tdh
Ordered Locus Names:	Fphi_0217

Organism

Francisella philomiragia subsp. philomiragia (strain ATCC 25017) [Complete proteome] [HAMAP]

Taxonomic identifier

484022 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Thiotrichales › Francisellaceae › Francisella

Protein attributes

Sequence length	351 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-threonine + NAD+ = L-2-amino-3-oxobutanoate + NADH. HAMAP MF_00627
Cofactor	Binds 2 zinc ions per subunit By similarity.
Pathway	Amino-acid degradation; L-threonine degradation via oxydo-reductase pathway; glycine from L-threonine: step 1/2. HAMAP MF_00627
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the zinc-containing alcohol dehydrogenase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding NAD Zinc
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW threonine catabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-threonine 3-dehydrogenase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 351	351	L-threonine 3-dehydrogenase HAMAP MF_00627		PRO_1000082611
Sites
	Metal binding	39	1	Zinc 1; catalytic By similarity
	Metal binding	64	1	Zinc 1; catalytic By similarity
	Metal binding	94	1	Zinc 2 By similarity
	Metal binding	97	1	Zinc 2 By similarity
	Metal binding	100	1	Zinc 2 By similarity
	Metal binding	108	1	Zinc 2 By similarity
	Metal binding	149	1	Zinc 1; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	B0TYR8-1 [UniParc]. Last modified April 8, 2008. Version 1. Checksum: 24BA37258CDDC23D	FASTA	351	38,452
	10 20 30 40 50 60 MKALAKLKKE PGIWMIDDAP MPEYGYNDVL IKIKKTAICG TDLHIYNWDK WSQATIPVPM 70 80 90 100 110 120 ITGHEFAGEV VAKGNGVTSV DVGDRVSGEG HLVCGQCRNC RAGKRHLCRK TIGIGVNVQG 130 140 150 160 170 180 AFAEYLVMPA VNVFKIPDSI SDDIASTFDP MGNAIHTALS FNLTGEDVLI TGAGPIGLMA 190 200 210 220 230 240 VKIARFCGAR RIVITDINKY RLQMARDFGA TVAVNVSEFQ NQQQLTAQMR KVMSEIGMTE 250 260 270 280 290 300 GFDVGLEMSG INSAISMMLD VMNHGGKLSL LGISAGDISV DWGAILFKGL TLKGIYGREM 310 320 330 340 350 FETWYLMTSM LQAGMDMEPI ITHRLHIDDY QKGFEIMKSG QCGKVILDWS R

References

[1]

"Complete sequence of chromosome of Francisella philomiragia subsp. philomiragia ATCC 25017." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Richardson P. Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000937 Genomic DNA. Translation: ABZ86433.1.
RefSeq	YP_001676934.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5907794.
GenomeReviews	Gene locus Fphi_0217 in contig CP000937_GR.
KEGG	fph:Fphi_0217.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	WMIDDAP.
Family and domain databases
HAMAP	MF_00627. [Tree]
InterPro	IPR013154. ADH_GroES-like. IPR002085. ADH_SF_Zn. IPR013149. ADH_Zn-bd. IPR002328. ADH_Zn_CS. IPR004627. L-Threonine_3-DHase. [Graphical view]
PANTHER	PTHR11695. ADH_Sf_Zn. 1 hit.
Pfam	PF08240. ADH_N. 1 hit. PF00107. ADH_zinc_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00692. tdh. 1 hit.
PROSITE	PS00059. ADH_ZINC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TDH_FRAP2

Accession

Primary (citable) accession number: B0TYR8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	April 8, 2008
Last modified:	November 3, 2009
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)