B0TXY0 (GLO2_FRAP2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hydroxyacylglutathione hydrolase
EC=3.1.2.6

Alternative name(s):
Glyoxalase II
Short name=Glx II

Gene names

Name:	gloB
Ordered Locus Names:	Fphi_1316

Organism

Francisella philomiragia subsp. philomiragia (strain ATCC 25017) [Complete proteome] [HAMAP]

Taxonomic identifier

484022 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Thiotrichales › Francisellaceae › Francisella

Protein attributes

Sequence length	252 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid By similarity. HAMAP MF_01374
Catalytic activity	S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate. HAMAP MF_01374
Cofactor	Binds 2 zinc ions per subunit By similarity. HAMAP MF_01374
Pathway	Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 2/2. HAMAP MF_01374
Subunit structure	Monomer By similarity. HAMAP MF_01374
Sequence similarities	Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	hydroxyacylglutathione hydrolase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 252	252	Hydroxyacylglutathione hydrolase HAMAP MF_01374		PRO_1000087280
Sites
	Metal binding	54	1	Zinc 1 By similarity
	Metal binding	56	1	Zinc 1 By similarity
	Metal binding	58	1	Zinc 2 By similarity
	Metal binding	59	1	Zinc 2 By similarity
	Metal binding	111	1	Zinc 1 By similarity
	Metal binding	130	1	Zinc 1 By similarity
	Metal binding	130	1	Zinc 2 By similarity
	Metal binding	170	1	Zinc 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	B0TXY0 [UniParc]. Last modified April 8, 2008. Version 1. Checksum: 170D5841DCF11D76	FASTA	252	28,949
	10 20 30 40 50 60 MQVKRWFLNN SLRNYQYLLY DENYAIVIDP LKADIFDEFI KQNTLKLEAI LITHRHGDHI 70 80 90 100 110 120 AGVKKLLEIY PDALVYAYAD NELFKPNIYV ADGDFVDFGF TSCKVMYTPG HIDDHVCFLF 130 140 150 160 170 180 EKEKALFCGD TLFNAGVGGV HAASADVNQL YDSVVKISSL DGDIKPYPAH DYWQSNLDFA 190 200 210 220 230 240 LSILPNDESF NYYRNQVAEL AAEHKPVVNL AEESKLNIFI RSISDKSLLQ ALPEYRLGRE 250 MFVKLRKLKN NF

References

[1]

"Complete sequence of chromosome of Francisella philomiragia subsp. philomiragia ATCC 25017." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Richardson P. Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25017.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000937 Genomic DNA. Translation: ABZ87541.1.
RefSeq	YP_001678042.1. NC_010336.1.
3D structure databases
ProteinModelPortal	B0TXY0.
ModBase	Search...
Protein-protein interaction databases
STRING	B0TXY0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5907518.
GenomeReviews	Gene locus Fphi_1316 in contig CP000937_GR.
KEGG	fph:Fphi_1316.
PATRIC	17939570. VBIFraPhi43880_1365.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG753931.
OMA	QEYAANA.
ProtClustDB	CLSK935013.
Enzyme and pathway databases
BioCyc	FPHI484022:FPHI_1316-MONOMER.
Family and domain databases
HAMAP	MF_01374. Glyoxalase_2. [Tree]
InterPro	IPR001279. Beta-lactamas-like. IPR017782. Hydroxyacylglutathione_Hdrlase. [Graphical view]
KO	K01069.
PANTHER	PTHR11935:SF7. PTHR11935:SF7. 1 hit.
Pfam	PF00753. Lactamase_B. 1 hit. [Graphical view]
SMART	SM00849. Lactamase_B. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLO2_FRAP2

Accession

Primary (citable) accession number: B0TXY0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	April 8, 2008
Last modified:	January 25, 2012
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program