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B0TXQ5 (TRMB_FRAP2) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 42. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:Fphi_1288
OrganismFrancisella philomiragia subsp. philomiragia (strain ATCC 25017) [Complete proteome] [HAMAP]
Taxonomic identifier484022 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaThiotrichalesFrancisellaceaeFrancisella

Protein attributes

Sequence length229 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 229229tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_1000084442

Regions

Region143 – 1486Interaction with RNA Potential
Region208 – 2114Substrate binding By similarity

Sites

Active site1371 By similarity
Binding site621S-adenosyl-L-methionine By similarity
Binding site871S-adenosyl-L-methionine By similarity
Binding site1141S-adenosyl-L-methionine By similarity
Binding site1371S-adenosyl-L-methionine By similarity
Binding site1411Substrate By similarity
Binding site1731Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
B0TXQ5 [UniParc].

Last modified April 8, 2008. Version 1.
Checksum: DA6E298D37BA2C38

FASTA22926,515
        10         20         30         40         50         60 
MSDNSKENLR QIKSYVQRAG RVTKKQQQAL DDYASKYMIE YDQNKSLDFS EIFKNSNDVV 

        70         80         90        100        110        120 
LEIGFGMGGS LVQMALENPT KNYLGIEVHK AGVGNILYEI EHQNISNLLV MSHDAVEILE 

       130        140        150        160        170        180 
NMISDNSLSG MQIYFPDPWH KKKHNKRRLV NQSNVDLFAK KLKVGGVFHY ASDWLPYAEE 

       190        200        210        220 
VLELLENDNK YKNLYDGFAP RPEWRPLTKF EKRGQNLDHP ISDILFEKI 

« Hide

References

[1]"Complete sequence of chromosome of Francisella philomiragia subsp. philomiragia ATCC 25017."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Richardson P.
Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 25017.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000937 Genomic DNA. Translation: ABZ87513.1.
RefSeqYP_001678014.1. NC_010336.1.

3D structure databases

ProteinModelPortalB0TXQ5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING484022.Fphi_1288.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABZ87513; ABZ87513; Fphi_1288.
GeneID5908856.
KEGGfph:Fphi_1288.
PATRIC17939510. VBIFraPhi43880_1336.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000073968.
KOK03439.
OMADNYWPVM.
OrthoDBEOG6K6VBC.
ProtClustDBPRK00121.

Enzyme and pathway databases

BioCycFPHI484022:GHVB-1325-MONOMER.
UniPathwayUPA00989.

Family and domain databases

HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_FRAP2
AccessionPrimary (citable) accession number: B0TXQ5
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: April 8, 2008
Last modified: April 16, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways