B0TUC0 (E4PD_SHEHH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
D-erythrose-4-phosphate dehydrogenase
Short name=E4PDH
EC=1.2.1.72

Gene names

Name:	epd
Ordered Locus Names:	Shal_0845

Organism

Shewanella halifaxensis (strain HAW-EB4) [Complete proteome] [HAMAP]

Taxonomic identifier

458817 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	339 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NAD-dependent conversion of D-erythrose 4-phosphate to 4-phosphoerythronate By similarity. HAMAP MF_01640
Catalytic activity	D-erythrose 4-phosphate + NAD+ + H2O = 4-phosphoerythronate + NADH. HAMAP MF_01640
Pathway	Cofactor biosynthesis; pyridoxine 5'-phosphate biosynthesis; pyridoxine 5'-phosphate from D-erythrose 4-phosphate: step 1/5. HAMAP MF_01640
Subunit structure	Homotetramer By similarity. HAMAP MF_01640
Subcellular location	Cytoplasm By similarity HAMAP MF_01640.
Sequence similarities	Belongs to the glyceraldehyde-3-phosphate dehydrogenase family. Epd subfamily.

Ontologies

Keywords
Biological process	Pyridoxine biosynthesis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: InterPro pyridoxal phosphate biosynthetic process Inferred from electronic annotation. Source: InterPro pyridoxine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro NADP binding Inferred from electronic annotation. Source: InterPro erythrose-4-phosphate dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 339	339	D-erythrose-4-phosphate dehydrogenase HAMAP MF_01640		PRO_1000088209
Regions
	Nucleotide binding	11 – 12	2	NAD By similarity
	Region	153 – 155	3	Substrate binding Potential
	Region	212 – 213	2	Substrate binding Potential
Sites
	Active site	154	1	Nucleophile By similarity
	Binding site	199	1	Substrate Potential
	Binding site	235	1	Substrate Potential
	Binding site	317	1	NAD By similarity
	Site	181	1	Activates thiol group during catalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	B0TUC0 [UniParc]. Last modified April 8, 2008. Version 1. Checksum: 2C0081DF3286173C	FASTA	339	37,808
	10 20 30 40 50 60 MIRVAINGYG RIGRSILRAL YESAKRDRIQ IVAINELAKP EAMLHLTQYD TTHGRFHTQV 70 80 90 100 110 120 KLDNQHMIIG DDAIKLLHEP NPANLPWKEL DIDIVYEATG VINDRQECEA HLKAGAKQVL 130 140 150 160 170 180 ISHPSSNDVD ATIVYGVNQD LLRAEHTVVS NASCTTNCIV PVIDVLDRHF EVKSGAITTI 190 200 210 220 230 240 HSAMNDQQVI DAYHDDLRRT RAAGQSIIPV DTKLARGIER ILPHMKNKFE AISVRVPTIN 250 260 270 280 290 300 VTAIDLSVTL NKRVDIELVN RVLKQATEGS FSGVVGFTNE PLVSCDFNHD PRSSIVDGTQ 310 320 330 TRVSDGHLVK LLLWCDNEWG FANRMLDTSL EMIKAKRSS

References

[1]

"Complete sequence of Shewanella halifaxensis HAW-EB4." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Kim E., Zhao J.-S., Richardson P. Submitted (JAN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HAW-EB4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000931 Genomic DNA. Translation: ABZ75420.1.
RefSeq	YP_001673079.1. NC_010334.1.
3D structure databases
ProteinModelPortal	B0TUC0.
ModBase	Search...
Protein-protein interaction databases
STRING	B0TUC0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5902965.
GenomeReviews	Gene locus Shal_0845 in contig CP000931_GR.
KEGG	shl:Shal_0845.
PATRIC	23504188. VBISheHal24697_0890.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG571736.
OMA	TTHGRFQ.
ProtClustDB	PRK13535.
Enzyme and pathway databases
BioCyc	SHAL458817:SHAL_0845-MONOMER.
Family and domain databases
HAMAP	MF_01640. E4P_dehydrog. [Tree]
InterPro	IPR006422. E4P_DH_bac. IPR020831. GlycerAld/Erythrose_P_DH. IPR020830. GlycerAld_3-P_DH_AS. IPR020829. GlycerAld_3-P_DH_cat. IPR020828. GlycerAld_3-P_DH_NAD(P)-bd. IPR006424. Glyceraldehyde-3-P_DH_1. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00134.
PANTHER	PTHR10836. GAP_DH. 1 hit. PTHR10836:SF24. PTHR10836:SF24. 1 hit.
Pfam	PF02800. Gp_dh_C. 1 hit. PF00044. Gp_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000149. GAP_DH. 1 hit.
PRINTS	PR00078. G3PDHDRGNASE.
SMART	SM00846. Gp_dh_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01532. E4PD_g-proteo. 1 hit. TIGR01534. GAPDH-I. 1 hit.
PROSITE	PS00071. GAPDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

E4PD_SHEHH

Accession

Primary (citable) accession number: B0TUC0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	April 8, 2008
Last modified:	January 25, 2012
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program