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Protein

Sulfite reductase [NADPH] hemoprotein beta-component

Gene

cysI

Organism
Shewanella halifaxensis (strain HAW-EB4)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate.UniRule annotation

Catalytic activityi

H2S + 3 NADP+ + 3 H2O = sulfite + 3 NADPH.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • sirohemeUniRule annotationNote: Binds 1 siroheme per subunit.UniRule annotation
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per subunit.UniRule annotation

Pathwayi: hydrogen sulfide biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes hydrogen sulfide from sulfite (NADPH route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Sulfite reductase [NADPH] flavoprotein alpha-component (Shal_3543), Sulfite reductase [NADPH] hemoprotein beta-component (cysI)
This subpathway is part of the pathway hydrogen sulfide biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes hydrogen sulfide from sulfite (NADPH route), the pathway hydrogen sulfide biosynthesis and in Sulfur metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi429 – 4291Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi435 – 4351Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi474 – 4741Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi478 – 4781Iron (siroheme axial ligand)UniRule annotation
Metal bindingi478 – 4781Iron-sulfur (4Fe-4S)UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Cysteine biosynthesis

Keywords - Ligandi

4Fe-4S, Heme, Iron, Iron-sulfur, Metal-binding, NADP

Enzyme and pathway databases

BioCyciSHAL458817:GH1X-3661-MONOMER.
UniPathwayiUPA00140; UER00207.

Names & Taxonomyi

Protein namesi
Recommended name:
Sulfite reductase [NADPH] hemoprotein beta-componentUniRule annotation (EC:1.8.1.2UniRule annotation)
Short name:
SiR-HPUniRule annotation
Short name:
SiRHPUniRule annotation
Gene namesi
Name:cysIUniRule annotation
Ordered Locus Names:Shal_3542
OrganismiShewanella halifaxensis (strain HAW-EB4)
Taxonomic identifieri458817 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
Proteomesi
  • UP000001317 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 565565Sulfite reductase [NADPH] hemoprotein beta-componentPRO_0000388514Add
BLAST

Interactioni

Subunit structurei

Alpha(8)-beta8. The alpha component is a flavoprotein, the beta component is a hemoprotein.UniRule annotation

Protein-protein interaction databases

STRINGi458817.Shal_3542.

Structurei

3D structure databases

ProteinModelPortaliB0TTE4.
SMRiB0TTE4. Positions 72-564.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105ET1. Bacteria.
COG0155. LUCA.
HOGENOMiHOG000218418.
KOiK00381.
OMAiMGMTHGD.
OrthoDBiEOG6W9XFB.

Family and domain databases

Gene3Di3.90.480.10. 2 hits.
HAMAPiMF_01540. CysI.
InterProiIPR011786. CysI.
IPR005117. NiRdtase/SiRdtase_haem-b_fer.
IPR006067. NO2/SO3_Rdtase_4Fe4S_dom.
IPR006066. NO2/SO3_Rdtase_FeS/sirohaem_BS.
[Graphical view]
PfamiPF01077. NIR_SIR. 2 hits.
PF03460. NIR_SIR_ferr. 2 hits.
[Graphical view]
PRINTSiPR00397. SIROHAEM.
SUPFAMiSSF55124. SSF55124. 2 hits.
TIGRFAMsiTIGR02041. CysI. 1 hit.
PROSITEiPS00365. NIR_SIR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B0TTE4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEQKLAVNE YLKTDSDYLR GTIQQGLNTA VTGAFSEGDQ QLIKFHGFYQ
60 70 80 90 100
QDDRDLRNER KEQKLEPLYS FMLRARVAGG VCSPEQWLAV DKIASNLTSA
110 120 130 140 150
NSIRLTTRQT FQYHGIPKRN LKTIIQDLDR EALDSIAACG DVNRNVMCNP
160 170 180 190 200
NPVESKLHQQ AYAYAKQLSD NMLPHTKAYA EIWLDNEKLV TTEGEEVEPV
210 220 230 240 250
YGQTYLPRKF KMAVAVPPDN DVDVYTNDLG FIAVAEGDEL VGFNMVAGGG
260 270 280 290 300
MGSTHGEVST FPRLADDFGY IKAEDSLKFA EAVMTIQRDW GNRENRKLSR
310 320 330 340 350
LKYTIVKHGF ETFKAEIEAR TGIKFEPRRD VVIGDRGDRY GWKQGVDDNW
360 370 380 390 400
HLTLFIEGGR VKDLPGQPLQ TGLREIAKIH RGDFRMTSNQ NMIIAGVASA
410 420 430 440 450
DKEQIEGLAR QYGLLGKLIT ETRGHSIACV ALPTCALAMA EAERYFPDFL
460 470 480 490 500
TKVEALQQKH GFLDQGIVIR MTGCPNGCAR PFAAEIGLVG KAPGRYNLYL
510 520 530 540 550
GASFEGTRLN KLYRENIQEA EILDQLDTLF AQYASQRQAG ETFGNYTVRS
560
GVVAAVNDAA KDFHG
Length:565
Mass (Da):63,081
Last modified:April 8, 2008 - v1
Checksum:i288C1BB66C7C1782
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000931 Genomic DNA. Translation: ABZ78085.1.
RefSeqiWP_012278605.1. NC_010334.1.

Genome annotation databases

EnsemblBacteriaiABZ78085; ABZ78085; Shal_3542.
KEGGishl:Shal_3542.
PATRICi23510046. VBISheHal24697_3710.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000931 Genomic DNA. Translation: ABZ78085.1.
RefSeqiWP_012278605.1. NC_010334.1.

3D structure databases

ProteinModelPortaliB0TTE4.
SMRiB0TTE4. Positions 72-564.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi458817.Shal_3542.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABZ78085; ABZ78085; Shal_3542.
KEGGishl:Shal_3542.
PATRICi23510046. VBISheHal24697_3710.

Phylogenomic databases

eggNOGiENOG4105ET1. Bacteria.
COG0155. LUCA.
HOGENOMiHOG000218418.
KOiK00381.
OMAiMGMTHGD.
OrthoDBiEOG6W9XFB.

Enzyme and pathway databases

UniPathwayiUPA00140; UER00207.
BioCyciSHAL458817:GH1X-3661-MONOMER.

Family and domain databases

Gene3Di3.90.480.10. 2 hits.
HAMAPiMF_01540. CysI.
InterProiIPR011786. CysI.
IPR005117. NiRdtase/SiRdtase_haem-b_fer.
IPR006067. NO2/SO3_Rdtase_4Fe4S_dom.
IPR006066. NO2/SO3_Rdtase_FeS/sirohaem_BS.
[Graphical view]
PfamiPF01077. NIR_SIR. 2 hits.
PF03460. NIR_SIR_ferr. 2 hits.
[Graphical view]
PRINTSiPR00397. SIROHAEM.
SUPFAMiSSF55124. SSF55124. 2 hits.
TIGRFAMsiTIGR02041. CysI. 1 hit.
PROSITEiPS00365. NIR_SIR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HAW-EB4.

Entry informationi

Entry nameiCYSI_SHEHH
AccessioniPrimary (citable) accession number: B0TTE4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 3, 2009
Last sequence update: April 8, 2008
Last modified: December 9, 2015
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.