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Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Shewanella halifaxensis (strain HAW-EB4)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.UniRule annotation

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation
  • Mo-bis(molybdopterin guanine dinucleotide)UniRule annotationNote: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi46 – 461Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi49 – 491Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi53 – 531Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi81 – 811Iron-sulfur (4Fe-4S)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. electron carrier activity Source: UniProtKB-HAMAP
  3. iron ion binding Source: UniProtKB-HAMAP
  4. molybdenum ion binding Source: InterPro
  5. nitrate reductase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
  2. nitrate assimilation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciSHAL458817:GH1X-731-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductaseUniRule annotation (EC:1.7.99.4UniRule annotation)
Gene namesi
Name:napAUniRule annotation
Ordered Locus Names:Shal_0715
OrganismiShewanella halifaxensis (strain HAW-EB4)
Taxonomic identifieri458817 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
ProteomesiUP000001317 Componenti: Chromosome

Subcellular locationi

Periplasm UniRule annotation

GO - Cellular componenti

  1. periplasmic space Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3232Tat-type signalUniRule annotationAdd
BLAST
Chaini33 – 826794Periplasmic nitrate reductasePRO_1000088120Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

Interactioni

Subunit structurei

Interacts with NapB.UniRule annotation

Protein-protein interaction databases

STRINGi458817.Shal_0715.

Structurei

3D structure databases

SMRiB0TSW5. Positions 39-825.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini39 – 95574Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation
Contains 1 4Fe-4S Mo/W bis-MGD-type domain.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031441.
KOiK02567.
OMAiESPVNKG.
OrthoDBiEOG6CVV7G.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B0TSW5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSISRREFLK ANAAVAAATA VGATLPVKIV EAAEQKDNIK WDKAPCRFCG
60 70 80 90 100
VGCSVLVGTD NGKVVATKGD PESPVNKGLN CIKGYFLSKI MYGKDRLTTP
110 120 130 140 150
LLRMKDGQYD KEGEFTPISW DSAFDIMADK WKDTLKTKGP TAVGMFGSGQ
160 170 180 190 200
WTVWEGYAAS KLHKAGFLTN NIDPNARHCM ASAVGGFMRT FGIDEPMGCY
210 220 230 240 250
DDLEAADQFV LWGANMAEMH PILWARLSDR RLSSPTSRVH VLSTYENRSF
260 270 280 290 300
DLADNAMVFR PQSDLVILNY VANYIIQNDA VNKDFVNKHT KFALGTTDIG
310 320 330 340 350
YGLRPDHPLE KKAKNPGNGK SKPISFDEYA KFVSSYTLEY AAEMSGVEPE
360 370 380 390 400
KLELMAKAYA DPNVKMMSLW TMGINQHTRG VWANNMLYNI HLLTGKIATP
410 420 430 440 450
GNSPFSLTGQ PSACGTAREV GTFAHRLPAD MVVANPKHRE ITEKLWQVPA
460 470 480 490 500
GTIPPKPGFH AVLQSRMLKD GKLNCYWTMC TNNMQAGPNI NDEMYPGFRN
510 520 530 540 550
PENFIVVSDP YPTVTAMAAD LILPTAMWVE KEGAYGNAER RTHMWHQQVK
560 570 580 590 600
APEGAKSDLW QLMEFSKRFK VSEVWPAELI AKQPELADKT LFDVLYANGV
610 620 630 640 650
VDKFPSSECK GQYNDEADAF GFYVQKGLFE EYAQFGRGHA HDLADFDTYH
660 670 680 690 700
ETRGLRWPVV DGKETLRRFS KGDPYVKGDK EFDFYGKPDG KAVIFALPFE
710 720 730 740 750
PAAEEPNEEY DIWLSTGRVL EHWHTGSMTA RVPELYRAYP DAQIFMHPED
760 770 780 790 800
AKARGLKRGD EVIVASPRGE VKTRVETKGR NKPPRGVAFM PFFDARQLVN
810 820
KLILDATDPL SKETDFKKCP VKVMKA
Length:826
Mass (Da):92,339
Last modified:April 8, 2008 - v1
Checksum:i40B787D80A241A78
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000931 Genomic DNA. Translation: ABZ75290.1.
RefSeqiWP_012275844.1. NC_010334.1.
YP_001672949.1. NC_010334.1.

Genome annotation databases

EnsemblBacteriaiABZ75290; ABZ75290; Shal_0715.
KEGGishl:Shal_0715.
PATRICi23503910. VBISheHal24697_0751.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000931 Genomic DNA. Translation: ABZ75290.1.
RefSeqiWP_012275844.1. NC_010334.1.
YP_001672949.1. NC_010334.1.

3D structure databases

SMRiB0TSW5. Positions 39-825.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi458817.Shal_0715.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABZ75290; ABZ75290; Shal_0715.
KEGGishl:Shal_0715.
PATRICi23503910. VBISheHal24697_0751.

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031441.
KOiK02567.
OMAiESPVNKG.
OrthoDBiEOG6CVV7G.

Enzyme and pathway databases

BioCyciSHAL458817:GH1X-731-MONOMER.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HAW-EB4.

Entry informationi

Entry nameiNAPA_SHEHH
AccessioniPrimary (citable) accession number: B0TSW5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: April 8, 2008
Last modified: April 1, 2015
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.