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B0TSW5

- NAPA_SHEHH

UniProt

B0TSW5 - NAPA_SHEHH

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Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Shewanella halifaxensis (strain HAW-EB4)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.UniRule annotation

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster.UniRule annotation
Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi46 – 461Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi49 – 491Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi53 – 531Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi81 – 811Iron-sulfur (4Fe-4S)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. electron carrier activity Source: UniProtKB-HAMAP
  3. iron ion binding Source: UniProtKB-HAMAP
  4. molybdenum ion binding Source: InterPro
  5. nitrate reductase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
  2. nitrate assimilation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciSHAL458817:GH1X-731-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductaseUniRule annotation (EC:1.7.99.4UniRule annotation)
Gene namesi
Name:napAUniRule annotation
Ordered Locus Names:Shal_0715
OrganismiShewanella halifaxensis (strain HAW-EB4)
Taxonomic identifieri458817 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
ProteomesiUP000001317: Chromosome

Subcellular locationi

Periplasm UniRule annotation

GO - Cellular componenti

  1. periplasmic space Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3232Tat-type signalUniRule annotationAdd
BLAST
Chaini33 – 826794Periplasmic nitrate reductasePRO_1000088120Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

Interactioni

Subunit structurei

Interacts with NapB.UniRule annotation

Protein-protein interaction databases

STRINGi458817.Shal_0715.

Structurei

3D structure databases

ProteinModelPortaliB0TSW5.
SMRiB0TSW5. Positions 39-825.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini39 – 95574Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation
Contains 1 4Fe-4S Mo/W bis-MGD-type domain.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031441.
KOiK02567.
OMAiRRLSHKD.
OrthoDBiEOG6CVV7G.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B0TSW5 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSISRREFLK ANAAVAAATA VGATLPVKIV EAAEQKDNIK WDKAPCRFCG
60 70 80 90 100
VGCSVLVGTD NGKVVATKGD PESPVNKGLN CIKGYFLSKI MYGKDRLTTP
110 120 130 140 150
LLRMKDGQYD KEGEFTPISW DSAFDIMADK WKDTLKTKGP TAVGMFGSGQ
160 170 180 190 200
WTVWEGYAAS KLHKAGFLTN NIDPNARHCM ASAVGGFMRT FGIDEPMGCY
210 220 230 240 250
DDLEAADQFV LWGANMAEMH PILWARLSDR RLSSPTSRVH VLSTYENRSF
260 270 280 290 300
DLADNAMVFR PQSDLVILNY VANYIIQNDA VNKDFVNKHT KFALGTTDIG
310 320 330 340 350
YGLRPDHPLE KKAKNPGNGK SKPISFDEYA KFVSSYTLEY AAEMSGVEPE
360 370 380 390 400
KLELMAKAYA DPNVKMMSLW TMGINQHTRG VWANNMLYNI HLLTGKIATP
410 420 430 440 450
GNSPFSLTGQ PSACGTAREV GTFAHRLPAD MVVANPKHRE ITEKLWQVPA
460 470 480 490 500
GTIPPKPGFH AVLQSRMLKD GKLNCYWTMC TNNMQAGPNI NDEMYPGFRN
510 520 530 540 550
PENFIVVSDP YPTVTAMAAD LILPTAMWVE KEGAYGNAER RTHMWHQQVK
560 570 580 590 600
APEGAKSDLW QLMEFSKRFK VSEVWPAELI AKQPELADKT LFDVLYANGV
610 620 630 640 650
VDKFPSSECK GQYNDEADAF GFYVQKGLFE EYAQFGRGHA HDLADFDTYH
660 670 680 690 700
ETRGLRWPVV DGKETLRRFS KGDPYVKGDK EFDFYGKPDG KAVIFALPFE
710 720 730 740 750
PAAEEPNEEY DIWLSTGRVL EHWHTGSMTA RVPELYRAYP DAQIFMHPED
760 770 780 790 800
AKARGLKRGD EVIVASPRGE VKTRVETKGR NKPPRGVAFM PFFDARQLVN
810 820
KLILDATDPL SKETDFKKCP VKVMKA
Length:826
Mass (Da):92,339
Last modified:April 8, 2008 - v1
Checksum:i40B787D80A241A78
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000931 Genomic DNA. Translation: ABZ75290.1.
RefSeqiWP_012275844.1. NC_010334.1.
YP_001672949.1. NC_010334.1.

Genome annotation databases

EnsemblBacteriaiABZ75290; ABZ75290; Shal_0715.
GeneIDi5906601.
KEGGishl:Shal_0715.
PATRICi23503910. VBISheHal24697_0751.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000931 Genomic DNA. Translation: ABZ75290.1 .
RefSeqi WP_012275844.1. NC_010334.1.
YP_001672949.1. NC_010334.1.

3D structure databases

ProteinModelPortali B0TSW5.
SMRi B0TSW5. Positions 39-825.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 458817.Shal_0715.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABZ75290 ; ABZ75290 ; Shal_0715 .
GeneIDi 5906601.
KEGGi shl:Shal_0715.
PATRICi 23503910. VBISheHal24697_0751.

Phylogenomic databases

eggNOGi COG0243.
HOGENOMi HOG000031441.
KOi K02567.
OMAi RRLSHKD.
OrthoDBi EOG6CVV7G.

Enzyme and pathway databases

BioCyci SHAL458817:GH1X-731-MONOMER.

Family and domain databases

HAMAPi MF_01630. Nitrate_reduct.
InterProi IPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view ]
Pfami PF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view ]
SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view ]
SUPFAMi SSF50692. SSF50692. 1 hit.
TIGRFAMsi TIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HAW-EB4.

Entry informationi

Entry nameiNAPA_SHEHH
AccessioniPrimary (citable) accession number: B0TSW5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: April 8, 2008
Last modified: October 29, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3