Reviewed, UniProtKB/Swiss-Prot B0TSW5 (NAPA_SHEHH)

Names and origin

Protein names

Recommended name:
    Periplasmic nitrate reductase
    EC=1.7.99.4

Gene names

Name:	napA
Ordered Locus Names:	Shal_0715

Organism

Shewanella halifaxensis (strain HAW-EB4) [Complete proteome] [HAMAP]

Taxonomic identifier

458817 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	826 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein napC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity.
Catalytic activity	Nitrite + acceptor = nitrate + reduced acceptor. HAMAP MF_01630
Cofactor	Binds 1 4Fe-4S cluster By similarity. Binds 1 molybdenum ion per subunit By similarity. Binds 2 molybdopterin guanine dinucleotide (MGD) groups per subunit By similarity.
Subunit structure	Interacts with napB By similarity.
Subcellular location	Periplasm By similarity.
Post-translational modification	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP MF_01630
Sequence similarities	Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily.

Ontologies

Keywords
Biological process	Electron transport Nitrate assimilation Transport
Cellular component	Periplasm
Domain	Signal
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding Molybdenum
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	Mo-molybdopterin cofactor biosynthetic process Inferred from electronic annotation. Source: HAMAP electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW nitrate assimilation Inferred from electronic annotation. Source: HAMAP transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP molybdenum ion binding Inferred from electronic annotation. Source: UniProtKB-KW nitrate reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 32	32	Tat-type signal Potential
	Chain	33 – 826	794	Periplasmic nitrate reductase HAMAP MF_01630		PRO_1000088120
Sites
	Metal binding	46	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	49	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	53	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	81	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	B0TSW5-1 [UniParc]. Last modified April 8, 2008. Version 1. Checksum: 40B787D80A241A78	FASTA	826	92,339
	10 20 30 40 50 60 MSISRREFLK ANAAVAAATA VGATLPVKIV EAAEQKDNIK WDKAPCRFCG VGCSVLVGTD 70 80 90 100 110 120 NGKVVATKGD PESPVNKGLN CIKGYFLSKI MYGKDRLTTP LLRMKDGQYD KEGEFTPISW 130 140 150 160 170 180 DSAFDIMADK WKDTLKTKGP TAVGMFGSGQ WTVWEGYAAS KLHKAGFLTN NIDPNARHCM 190 200 210 220 230 240 ASAVGGFMRT FGIDEPMGCY DDLEAADQFV LWGANMAEMH PILWARLSDR RLSSPTSRVH 250 260 270 280 290 300 VLSTYENRSF DLADNAMVFR PQSDLVILNY VANYIIQNDA VNKDFVNKHT KFALGTTDIG 310 320 330 340 350 360 YGLRPDHPLE KKAKNPGNGK SKPISFDEYA KFVSSYTLEY AAEMSGVEPE KLELMAKAYA 370 380 390 400 410 420 DPNVKMMSLW TMGINQHTRG VWANNMLYNI HLLTGKIATP GNSPFSLTGQ PSACGTAREV 430 440 450 460 470 480 GTFAHRLPAD MVVANPKHRE ITEKLWQVPA GTIPPKPGFH AVLQSRMLKD GKLNCYWTMC 490 500 510 520 530 540 TNNMQAGPNI NDEMYPGFRN PENFIVVSDP YPTVTAMAAD LILPTAMWVE KEGAYGNAER 550 560 570 580 590 600 RTHMWHQQVK APEGAKSDLW QLMEFSKRFK VSEVWPAELI AKQPELADKT LFDVLYANGV 610 620 630 640 650 660 VDKFPSSECK GQYNDEADAF GFYVQKGLFE EYAQFGRGHA HDLADFDTYH ETRGLRWPVV 670 680 690 700 710 720 DGKETLRRFS KGDPYVKGDK EFDFYGKPDG KAVIFALPFE PAAEEPNEEY DIWLSTGRVL 730 740 750 760 770 780 EHWHTGSMTA RVPELYRAYP DAQIFMHPED AKARGLKRGD EVIVASPRGE VKTRVETKGR 790 800 810 820 NKPPRGVAFM PFFDARQLVN KLILDATDPL SKETDFKKCP VKVMKA

References

[1]

"Complete sequence of Shewanella halifaxensis HAW-EB4." Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Kim E., Zhao J.-S., Richardson P. Submitted (JAN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000931 Genomic DNA. Translation: ABZ75290.1.
RefSeq	YP_001672949.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5906601.
GenomeReviews	Gene locus Shal_0715 in contig CP000931_GR.
KEGG	shl:Shal_0715.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	B0TSW5. NMLYNIH.
Family and domain databases
HAMAP	MF_01630. [Tree]
InterPro	IPR009010. Asp_de-COase-like_fold. IPR006656. Mopterin_OxRdtase. IPR006963. Mopterin_OxRdtase_Fe4S4. IPR006655. Mopterin_OxRdtase_prok_CS. IPR006657. MPT_dinuc_bd. IPR010051. NO3_reductase_lsu_periplasm. IPR006311. Tat. IPR019546. TAT_signal. IPR017909. Twin_arg_translocation_Tat. [Graphical view]
Gene3D	G3DSA:2.40.40.20. Asp_decarboxylase-like_fold. 1 hit.
Pfam	PF04879. Molybdop_Fe4S4. 1 hit. PF00384. Molybdopterin. 1 hit. PF01568. Molydop_binding. 1 hit. PF10518. TAT_signal. 1 hit. [Graphical view]
TIGRFAMs	TIGR01706. NAPA. 1 hit. TIGR01409. TAT_signal_seq. 1 hit.
PROSITE	PS00551. MOLYBDOPTERIN_PROK_1. 1 hit. PS00490. MOLYBDOPTERIN_PROK_2. False negative. PS00932. MOLYBDOPTERIN_PROK_3. False negative. PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAPA_SHEHH

Accession

Primary (citable) accession number: B0TSW5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	April 8, 2008
Last modified:	June 16, 2009
This is version 11 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)