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Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Shewanella halifaxensis (strain HAW-EB4)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase complex NapAB. Receives electrons from NapB and catalyzes the reduction of nitrate to nitrite.UniRule annotation

Catalytic activityi

2 ferrocytochrome + nitrate + 2 H+ = 2 ferricytochrome + nitrite.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation
  • Mo-bis(molybdopterin guanine dinucleotide)UniRule annotationNote: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi46Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi49Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi53Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi81Iron-sulfur (4Fe-4S)UniRule annotation1
Binding sitei83Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei150Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei175Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei179Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei372Mo-bis(molybdopterin guanine dinucleotide); via amide nitrogenUniRule annotation1
Binding sitei376Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei482Mo-bis(molybdopterin guanine dinucleotide); via amide nitrogenUniRule annotation1
Binding sitei531Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei558Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei792Substrate; via amide nitrogenUniRule annotation1
Binding sitei800Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei817Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Nitrate assimilation, Transport
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciSHAL458817:G1GB1-741-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductaseUniRule annotation (EC:1.9.6.1UniRule annotation)
Gene namesi
Name:napAUniRule annotation
Ordered Locus Names:Shal_0715
OrganismiShewanella halifaxensis (strain HAW-EB4)
Taxonomic identifieri458817 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
Proteomesi
  • UP000001317 Componenti: Chromosome

Subcellular locationi

  • Periplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 32Tat-type signalUniRule annotationAdd BLAST32
ChainiPRO_100008812033 – 826Periplasmic nitrate reductaseUniRule annotationAdd BLAST794

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.UniRule annotation

Interactioni

Subunit structurei

Component of the periplasmic nitrate reductase NapAB complex composed of NapA and NapB.UniRule annotation

Protein-protein interaction databases

STRINGi458817.Shal_0715

Structurei

3D structure databases

ProteinModelPortaliB0TSW5
SMRiB0TSW5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini39 – 954Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd BLAST57

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni212 – 219Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation8
Regioni262 – 264Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation3
Regioni508 – 509Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation2
Regioni716 – 725Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation10

Sequence similaritiesi

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4107QIW Bacteria
COG0243 LUCA
HOGENOMiHOG000031441
KOiK02567
OMAiANSMGTR
OrthoDBiPOG091H060P

Family and domain databases

HAMAPiMF_01630 Nitrate_reduct_NapA, 1 hit
InterProiView protein in InterPro
IPR009010 Asp_de-COase-like_dom_sf
IPR006657 MoPterin_dinucl-bd_dom
IPR006656 Mopterin_OxRdtase
IPR006963 Mopterin_OxRdtase_4Fe-4S_dom
IPR027467 MopterinOxRdtase_cofactor_BS
IPR010051 Periplasm_NO3_reductase_lsu
IPR006311 TAT_signal
IPR019546 TAT_signal_bac_arc
PANTHERiPTHR11615:SF123 PTHR11615:SF123, 1 hit
PfamiView protein in Pfam
PF04879 Molybdop_Fe4S4, 1 hit
PF00384 Molybdopterin, 1 hit
PF01568 Molydop_binding, 1 hit
PF10518 TAT_signal, 1 hit
SMARTiView protein in SMART
SM00926 Molybdop_Fe4S4, 1 hit
SUPFAMiSSF50692 SSF50692, 1 hit
TIGRFAMsiTIGR01706 NAPA, 1 hit
TIGR01409 TAT_signal_seq, 1 hit
PROSITEiView protein in PROSITE
PS51669 4FE4S_MOW_BIS_MGD, 1 hit
PS00551 MOLYBDOPTERIN_PROK_1, 1 hit
PS51318 TAT, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B0TSW5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSISRREFLK ANAAVAAATA VGATLPVKIV EAAEQKDNIK WDKAPCRFCG
60 70 80 90 100
VGCSVLVGTD NGKVVATKGD PESPVNKGLN CIKGYFLSKI MYGKDRLTTP
110 120 130 140 150
LLRMKDGQYD KEGEFTPISW DSAFDIMADK WKDTLKTKGP TAVGMFGSGQ
160 170 180 190 200
WTVWEGYAAS KLHKAGFLTN NIDPNARHCM ASAVGGFMRT FGIDEPMGCY
210 220 230 240 250
DDLEAADQFV LWGANMAEMH PILWARLSDR RLSSPTSRVH VLSTYENRSF
260 270 280 290 300
DLADNAMVFR PQSDLVILNY VANYIIQNDA VNKDFVNKHT KFALGTTDIG
310 320 330 340 350
YGLRPDHPLE KKAKNPGNGK SKPISFDEYA KFVSSYTLEY AAEMSGVEPE
360 370 380 390 400
KLELMAKAYA DPNVKMMSLW TMGINQHTRG VWANNMLYNI HLLTGKIATP
410 420 430 440 450
GNSPFSLTGQ PSACGTAREV GTFAHRLPAD MVVANPKHRE ITEKLWQVPA
460 470 480 490 500
GTIPPKPGFH AVLQSRMLKD GKLNCYWTMC TNNMQAGPNI NDEMYPGFRN
510 520 530 540 550
PENFIVVSDP YPTVTAMAAD LILPTAMWVE KEGAYGNAER RTHMWHQQVK
560 570 580 590 600
APEGAKSDLW QLMEFSKRFK VSEVWPAELI AKQPELADKT LFDVLYANGV
610 620 630 640 650
VDKFPSSECK GQYNDEADAF GFYVQKGLFE EYAQFGRGHA HDLADFDTYH
660 670 680 690 700
ETRGLRWPVV DGKETLRRFS KGDPYVKGDK EFDFYGKPDG KAVIFALPFE
710 720 730 740 750
PAAEEPNEEY DIWLSTGRVL EHWHTGSMTA RVPELYRAYP DAQIFMHPED
760 770 780 790 800
AKARGLKRGD EVIVASPRGE VKTRVETKGR NKPPRGVAFM PFFDARQLVN
810 820
KLILDATDPL SKETDFKKCP VKVMKA
Length:826
Mass (Da):92,339
Last modified:April 8, 2008 - v1
Checksum:i40B787D80A241A78
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000931 Genomic DNA Translation: ABZ75290.1
RefSeqiWP_012275844.1, NC_010334.1

Genome annotation databases

EnsemblBacteriaiABZ75290; ABZ75290; Shal_0715
KEGGishl:Shal_0715

Similar proteinsi

Entry informationi

Entry nameiNAPA_SHEHH
AccessioniPrimary (citable) accession number: B0TSW5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: April 8, 2008
Last modified: March 28, 2018
This is version 69 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
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Main funding by: National Institutes of Health