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B0TSW5 (NAPA_SHEHH) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 43. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Periplasmic nitrate reductase

EC=1.7.99.4
Gene names
Name:napA
Ordered Locus Names:Shal_0715
OrganismShewanella halifaxensis (strain HAW-EB4) [Complete proteome] [HAMAP]
Taxonomic identifier458817 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella

Protein attributes

Sequence length826 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. HAMAP-Rule MF_01630

Catalytic activity

Nitrite + acceptor = nitrate + reduced acceptor. HAMAP-Rule MF_01630

Cofactor

Binds 1 4Fe-4S cluster By similarity. HAMAP-Rule MF_01630

Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Subunit structure

Interacts with NapB By similarity. HAMAP-Rule MF_01630

Subcellular location

Periplasm By similarity HAMAP-Rule MF_01630.

Post-translational modification

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP-Rule MF_01630

Sequence similarities

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.

Contains 1 4Fe-4S Mo/W bis-MGD-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3232Tat-type signal Potential
Chain33 – 826794Periplasmic nitrate reductase HAMAP-Rule MF_01630
PRO_1000088120

Regions

Domain39 – 95574Fe-4S Mo/W bis-MGD-type

Sites

Metal binding461Iron-sulfur (4Fe-4S) By similarity
Metal binding491Iron-sulfur (4Fe-4S) By similarity
Metal binding531Iron-sulfur (4Fe-4S) By similarity
Metal binding811Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
B0TSW5 [UniParc].

Last modified April 8, 2008. Version 1.
Checksum: 40B787D80A241A78

FASTA82692,339
        10         20         30         40         50         60 
MSISRREFLK ANAAVAAATA VGATLPVKIV EAAEQKDNIK WDKAPCRFCG VGCSVLVGTD 

        70         80         90        100        110        120 
NGKVVATKGD PESPVNKGLN CIKGYFLSKI MYGKDRLTTP LLRMKDGQYD KEGEFTPISW 

       130        140        150        160        170        180 
DSAFDIMADK WKDTLKTKGP TAVGMFGSGQ WTVWEGYAAS KLHKAGFLTN NIDPNARHCM 

       190        200        210        220        230        240 
ASAVGGFMRT FGIDEPMGCY DDLEAADQFV LWGANMAEMH PILWARLSDR RLSSPTSRVH 

       250        260        270        280        290        300 
VLSTYENRSF DLADNAMVFR PQSDLVILNY VANYIIQNDA VNKDFVNKHT KFALGTTDIG 

       310        320        330        340        350        360 
YGLRPDHPLE KKAKNPGNGK SKPISFDEYA KFVSSYTLEY AAEMSGVEPE KLELMAKAYA 

       370        380        390        400        410        420 
DPNVKMMSLW TMGINQHTRG VWANNMLYNI HLLTGKIATP GNSPFSLTGQ PSACGTAREV 

       430        440        450        460        470        480 
GTFAHRLPAD MVVANPKHRE ITEKLWQVPA GTIPPKPGFH AVLQSRMLKD GKLNCYWTMC 

       490        500        510        520        530        540 
TNNMQAGPNI NDEMYPGFRN PENFIVVSDP YPTVTAMAAD LILPTAMWVE KEGAYGNAER 

       550        560        570        580        590        600 
RTHMWHQQVK APEGAKSDLW QLMEFSKRFK VSEVWPAELI AKQPELADKT LFDVLYANGV 

       610        620        630        640        650        660 
VDKFPSSECK GQYNDEADAF GFYVQKGLFE EYAQFGRGHA HDLADFDTYH ETRGLRWPVV 

       670        680        690        700        710        720 
DGKETLRRFS KGDPYVKGDK EFDFYGKPDG KAVIFALPFE PAAEEPNEEY DIWLSTGRVL 

       730        740        750        760        770        780 
EHWHTGSMTA RVPELYRAYP DAQIFMHPED AKARGLKRGD EVIVASPRGE VKTRVETKGR 

       790        800        810        820 
NKPPRGVAFM PFFDARQLVN KLILDATDPL SKETDFKKCP VKVMKA 

« Hide

References

[1]"Complete sequence of Shewanella halifaxensis HAW-EB4."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. expand/collapse author list , Kyrpides N., Kim E., Zhao J.-S., Richardson P.
Submitted (JAN-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: HAW-EB4.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000931 Genomic DNA. Translation: ABZ75290.1.
RefSeqYP_001672949.1. NC_010334.1.

3D structure databases

ProteinModelPortalB0TSW5.
SMRB0TSW5. Positions 39-825.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING458817.Shal_0715.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABZ75290; ABZ75290; Shal_0715.
GeneID5906601.
KEGGshl:Shal_0715.
PATRIC23503910. VBISheHal24697_0751.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0243.
HOGENOMHOG000031441.
KOK02567.
OMARRLSHKD.
OrthoDBEOG6CVV7G.
ProtClustDBPRK13532.

Enzyme and pathway databases

BioCycSHAL458817:GH1X-731-MONOMER.

Family and domain databases

HAMAPMF_01630. Nitrate_reduct.
InterProIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMSSF50692. SSF50692. 1 hit.
TIGRFAMsTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNAPA_SHEHH
AccessionPrimary (citable) accession number: B0TSW5
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: April 8, 2008
Last modified: February 19, 2014
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families