ID   MAO1_SHEHH              Reviewed;         562 AA.
AC   B0TRQ2;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   08-APR-2008, sequence version 1.
DT   16-JUN-2009, entry version 12.
DE   RecName: Full=NAD-dependent malic enzyme;
DE            Short=NAD-ME;
DE            EC=1.1.1.38;
GN   Name=sfcA; OrderedLocusNames=Shal_3267;
OS   Shewanella halifaxensis (strain HAW-EB4).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales;
OC   Shewanellaceae; Shewanella.
OX   NCBI_TaxID=458817;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E.,
RA   Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T.,
RA   Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M.,
RA   Hauser L., Kyrpides N., Kim E., Zhao J.-S., Richardson P.;
RT   "Complete sequence of Shewanella halifaxensis HAW-EB4.";
RL   Submitted (JAN-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY: (S)-malate + NAD(+) = pyruvate + CO(2) + NADH.
CC   -!- COFACTOR: Divalent metal cations. Prefers magnesium or manganese
CC       (By similarity).
CC   -!- SUBUNIT: Homotetramer (By similarity).
CC   -!- SIMILARITY: Belongs to the malic enzymes family.
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DR   EMBL; CP000931; ABZ77814.1; -; Genomic_DNA.
DR   RefSeq; YP_001675473.1; -.
DR   GeneID; 5903878; -.
DR   GenomeReviews; CP000931_GR; Shal_3267.
DR   KEGG; shl:Shal_3267; -.
DR   OMA; B0TRQ2; IQDNNET.
DR   GO; GO:0016619; F:malate dehydrogenase (oxaloacetate-decarbox...; IEA:HAMAP.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0051287; F:NAD or NADH binding; IEA:InterPro.
DR   GO; GO:0006108; P:malate metabolic process; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   HAMAP; MF_01619; -; 1.
DR   InterPro; IPR015884; Malic_enzyme_CS.
DR   InterPro; IPR012301; Malic_N.
DR   InterPro; IPR012302; Malic_NAD_bd.
DR   InterPro; IPR001891; Malic_OxRdtase.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   Pfam; PF00390; malic; 1.
DR   Pfam; PF03949; Malic_M; 1.
DR   PRINTS; PR00072; MALOXRDTASE.
DR   PROSITE; PS00331; MALIC_ENZYMES; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Metal-binding; NAD; Oxidoreductase.
FT   CHAIN         1    562       NAD-dependent malic enzyme.
FT                                /FTId=PRO_1000088075.
FT   ACT_SITE    101    101       Proton donor (By similarity).
FT   ACT_SITE    172    172       Proton acceptor (By similarity).
FT   METAL       243    243       Divalent metal cation (By similarity).
FT   METAL       244    244       Divalent metal cation (By similarity).
FT   METAL       267    267       Divalent metal cation (By similarity).
FT   BINDING     154    154       NAD (By similarity).
FT   BINDING     267    267       NAD (By similarity).
FT   BINDING     415    415       NAD (By similarity).
FT   SITE        267    267       Important for activity (By similarity).
SQ   SEQUENCE   562 AA;  62355 MW;  284A512F5FC46CD3 CRC64;
     MDDNKRPLYL PFAGPAILEA PLINKGSAFT EEERIFFNLE GLLPHVIETI EEQASRAYDQ
     YKNFSNDLDK HIYLRNIQDT NETLYYRLVQ NHITEMMPII YTPTVGMACE RFSKDYRRNR
     GLFISYANKD RIDDILNNST RQKVKIIVVT DGERILGLGD QGIGGMGIPI GKLSLYTSCG
     GISPAYTLPV TLDVGTDNPH LLEDPMYMGM RSPRIGGEEY KEFVEAFMQA VNRRWPDALI
     QFEDFAQKNA MPLLERYKDQ YCCFNDDIQG TAAVTVGSLL AACKAAKSKL SEQRITFLGA
     GSAGCGIAEA IVAQMVSEGI SEAQARKQVF MVDRWGMLQS NMPNLLPFQQ KLAQQCDDLT
     NWDNFSDNIS LLDVVNNAKP TVLIGVSGAP GLFTEEIIKA MHSHCPRPIV FPLSNPTSRV
     EATPKDILHW TQGKALVATG SPFEPVVIDD TTYEIAQCNN SYIFPGIGLG VLASGAKRVS
     DAMLMASSRA LAECSPLAID GEGSLLPKLE DIHLVSKRIA FAVARVAIEE GHALPTTKEL
     LTYAIEDNFW TAEYRSYKRT AF
//