B0TRN5 (KATG_SHEHH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Catalase-peroxidase
Short name=CP
EC=1.11.1.21

Alternative name(s):
Peroxidase/catalase

Gene names

Name:	katG
Ordered Locus Names:	Shal_1888

Organism

Shewanella halifaxensis (strain HAW-EB4) [Complete proteome] [HAMAP]

Taxonomic identifier

458817 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	718 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity. HAMAP MF_01961
Catalytic activity	Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961 2 H2O2 = O2 + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity. HAMAP MF_01961
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity. HAMAP MF_01961
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 718	718	Catalase-peroxidase HAMAP MF_01961		PRO_0000354919
Sites
	Active site	93	1	Proton acceptor By similarity
	Metal binding	261	1	Iron (heme axial ligand) By similarity
	Site	89	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	92 ↔ 220		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-246) By similarity
	Cross-link	220 ↔ 246		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-92) By similarity

Sequences

	Sequence		Length	Mass (Da)
	B0TRN5 [UniParc]. Last modified April 8, 2008. Version 1. Checksum: 1537EFDAD42FE9E6	FASTA	718	80,069
	10 20 30 40 50 60 MANKKCPVMH GGATEISMSN MEWWPKALNL DILHQHDSKT NPMGAGFNYR EQVKLLDVEA 70 80 90 100 110 120 LKKDLHALMT DSQPWWPADW GHYGGLMIRL SWHAAGTYRT ADGRGGAGRG NQRFAPLNSW 130 140 150 160 170 180 PDNVNLDKAR RLLWPLKKKY GNKLSWADLI AYAGTIAYES MGLKTFGFAF GREDIWHPEK 190 200 210 220 230 240 DIYWGAEKEW LAPSGSEGSR YSGERDLENP LASVMMGLIY VNPEGVDGHP DPLKTANDVR 250 260 270 280 290 300 VTFERMAMND EETVALTAGG HTVGKCHGNG DAELLGPVPE GADVEDQGLG WINKSQRGIG 310 320 330 340 350 360 RDTVSSGLEG AWTTHPTQWD NGYFTLLLNH EWQLNKSPAG AWQWEPINIK EEDKPVDVED 370 380 390 400 410 420 LTQRYNPIMT DADMAMKLDP DYQIISARFD KDPEYFSDVF ARTWFKLTHR DMGPKTRYIG 430 440 450 460 470 480 PDVPAVDLLW QDPVPKGRSD YDVEAVKAQI AASSLSVSDM VTTAWDSART FRGSDLRGGA 490 500 510 520 530 540 NGARIRLAPQ RDWQANEPER LNRVLAVLEP IAAKSGVSVA DVIVLAGNLA VEQAAKAAGF 550 560 570 580 590 600 AIKVPFSPGR GDATADMTDV ESFHVLEPLH DGYRNWLKKD YAVSAEELML DQTQLLGLTA 610 620 630 640 650 660 QEMTVLLGGM RVLGTNYNGE SHGAFTEHQG ALTNDFFVNL TDMNNTWQPV SNNLYEISDR 670 680 690 700 710 ETAKLKWTAT RVDLVFGSNS ILRAYAEVYA QDDNQQKFVE DFVTTWAKVM NSDRFDLD

References

[1]

"Complete sequence of Shewanella halifaxensis HAW-EB4." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Kim E., Zhao J.-S., Richardson P. Submitted (JAN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HAW-EB4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000931 Genomic DNA. Translation: ABZ76453.1.
RefSeq	YP_001674112.1. NC_010334.1.
3D structure databases
ProteinModelPortal	B0TRN5.
SMR	B0TRN5. Positions 19-718.
ModBase	Search...
Protein-protein interaction databases
STRING	B0TRN5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5903130.
GenomeReviews	Gene locus Shal_1888 in contig CP000931_GR.
KEGG	shl:Shal_1888.
PATRIC	23506467. VBISheHal24697_1981.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG285610.
OMA	WPNALNL.
ProtClustDB	PRK15061.
Enzyme and pathway databases
BioCyc	SHAL458817:SHAL_1888-MONOMER.
Family and domain databases
HAMAP	MF_01961. Catal-peroxid. [Tree]
InterPro	IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. [Graphical view]
KO	K03782.
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 2 hits.
TIGRFAMs	TIGR00198. Cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. False negative. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG_SHEHH

Accession

Primary (citable) accession number: B0TRN5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	April 8, 2008
Last modified:	January 25, 2012
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program