B0TR07 (RLMG_SHEHH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA large subunit methyltransferase G
EC=2.1.1.174

Alternative name(s):
23S rRNA m2G1835 methyltransferase
rRNA (guanine-N(2)-)-methyltransferase RlmG

Gene names

Name:	rlmG
Ordered Locus Names:	Shal_3191

Organism

Shewanella halifaxensis (strain HAW-EB4) [Complete proteome] [HAMAP]

Taxonomic identifier

458817 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	421 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the guanosine in position 1835 (m2G1835) of 23S rRNA By similarity. HAMAP MF_01859
Catalytic activity	S-adenosyl-L-methionine + guanosine(1835) in 23S rRNA = S-adenosyl-L-homocysteine + N(2)-methylguanosine(1835) in 23S rRNA. HAMAP MF_01859
Subcellular location	Cytoplasm Potential HAMAP MF_01859.
Sequence similarities	Belongs to the methyltransferase superfamily. RlmG family.
Sequence caution	The sequence ABZ77738.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nucleic acid binding Inferred from electronic annotation. Source: InterPro rRNA (guanine-N2-)-methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 421	421	Ribosomal RNA large subunit methyltransferase G HAMAP MF_01859		PRO_0000366510

Sequences

	Sequence		Length	Mass (Da)
	B0TR07 [UniParc]. Last modified March 24, 2009. Version 2. Checksum: C41D6019E6CE6B25	FASTA	421	46,654
	10 20 30 40 50 60 MTTQFSVSGI ELELLRYPSR QESNLQAWDA ADEHLIKHLI DTEQTNINTA IINDNFGALT 70 80 90 100 110 120 AGLLSIDPSW ALTLETDAKT SLLGTTQNLS RNQLPTDTLT WVNSCDELPQ GFELVLMKLP 130 140 150 160 170 180 KNLNYFSHQL NRLSHVLPAG TRVLIGAKAK SINKSLLETI EKNLGAASAS LTWKKTRVIT 190 200 210 220 230 240 CISDGKVRPL PKATTWSVPE FKLQITNLSN VFAANKLDIG ARIMLDNMPK GDFKSIVDLG 250 260 270 280 290 300 CGNGILGLHA KQVFPEAYIH FIDDSEMAVA SARENWALNK LDNPALVGEQ ATFGWDDCLT 310 320 330 340 350 360 HMSEGFRPDL ILCNPPFHQG EAITDHIAWQ MFLDAFRRLK NGGILHVVGN RHLAYHVKLQ 370 380 390 400 410 420 RIFKNCTTVA SNGKFVILQA QKISKKALEP ELEQESDLNS KLDANTEVPH PQSALYGKPK A

References

[1]

"Complete sequence of Shewanella halifaxensis HAW-EB4." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Kim E., Zhao J.-S., Richardson P. Submitted (JAN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HAW-EB4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000931 Genomic DNA. Translation: ABZ77738.1. Different initiation.
RefSeq	YP_001675397.1. NC_010334.1.
3D structure databases
ProteinModelPortal	B0TR07.
ModBase	Search...
Protein-protein interaction databases
STRING	B0TR07.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5903290.
GenomeReviews	Gene locus Shal_3191 in contig CP000931_GR.
KEGG	shl:Shal_3191.
PATRIC	23509281. VBISheHal24697_3346.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG321931.
ProtClustDB	CLSK906089.
Enzyme and pathway databases
BioCyc	SHAL458817:SHAL_3191-MONOMER.
Family and domain databases
HAMAP	MF_01859. 23SrRNA_methyltr_G. [Tree]
InterPro	IPR002052. DNA_methylase_N6_adenine_CS. IPR017237. rRNA_m2G-MeTrfase_RsmD. IPR007848. Small_mtfrase. [Graphical view]
KO	K00564.
Pfam	PF05175. MTS. 1 hit. [Graphical view]
PIRSF	PIRSF037565. RRNA_m2G_Mtase_RsmD_prd. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RLMG_SHEHH

Accession

Primary (citable) accession number: B0TR07

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	March 24, 2009
Last modified:	January 25, 2012
This is version 27 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program