B0TNU4 (PLSB_SHEHH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycerol-3-phosphate acyltransferase
Short name=GPAT
EC=2.3.1.15

Gene names

Name:	plsB
Ordered Locus Names:	Shal_0271

Organism

Shewanella halifaxensis (strain HAW-EB4) [Complete proteome] [HAMAP]

Taxonomic identifier

458817 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	807 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate. HAMAP MF_00393
Pathway	Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3. HAMAP MF_00393
Subcellular location	Cell inner membrane; Peripheral membrane protein; Cytoplasmic side By similarity HAMAP MF_00393.
Domain	The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate By similarity. HAMAP MF_00393
Sequence similarities	Belongs to the GPAT/DAPAT family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glycerol-3-phosphate O-acyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 807	807	Glycerol-3-phosphate acyltransferase HAMAP MF_00393		PRO_1000080292
Regions
	Motif	308 – 313	6	HXXXXD motif HAMAP MF_00393

Sequences

	Sequence		Length	Mass (Da)
	B0TNU4 [UniParc]. Last modified April 8, 2008. Version 1. Checksum: 1F2853AD80477A76	FASTA	807	91,709
	10 20 30 40 50 60 MSKQDSLWFK SLRWLQRKLV HTVVVPHDPF DDLNLDPSKP LVYVMKTESV SDIAALSEMT 70 80 90 100 110 120 EKLGLPSPYE ELEVNGIRAP RVVCLEGSKP LFGQREGGEQ YIDYFKRLLS VHKQNLELDI 130 140 150 160 170 180 QLVPVSLYWG RTPGKEDDTM RAAVLERQNP TWLRKCLMIL FLGRHNFVQF SNAVSLRYMA 190 200 210 220 230 240 DEHGTDKRIA QKLARVARAH FERQRKVMTG PQLPKRQALF HALLKSDSIT KAIKEEAASK 250 260 270 280 290 300 KISESEARAK AMEYLDEVAA DYSDSLVRIA ERFLTWLWNK LYKGINIKGA EQVRQLHHDG 310 320 330 340 350 360 HEIVYVPCHR SHMDYLLLSY ILYYQGMVPP HIAAGINLNF WPAGPAFRRG GAFFIRRSFG 370 380 390 400 410 420 GNKLYTAVFR EYLDQLFTKG YSVEYFTEGG RSRTGRLLAP KTGMLAMTLN SVLRGVERPV 430 440 450 460 470 480 TLVPVYLGYD HVMEVATYHK ELSGKKKKKE SVWQIFGAIR KLGNFGQGYV NFGEPITLHN 490 500 510 520 530 540 FLNEQVPSWR DDIAKDPDQK PTWLTPVVNT LANQVMTNIN DAAAVSSVTL TSMVLLASEQ 550 560 570 580 590 600 NALERSQLEK QLDLYLTLLK ERPYTDYTSV PDGTGHDLVS QGLELKKLQI ESDPLGDIIS 610 620 630 640 650 660 IDQSIAITMT YYRNNIIHLM VLPSLIAACL LRKENCGRND VISIVNDFYP LLEAELFMGI 670 680 690 700 710 720 EDPSQYANQI LDILVAQGLV VECDHFEVVD SSINQLLLLS GTISETMQRY AILFNLLEVK 730 740 750 760 770 780 PNMERSELEK DSHRLAQRLG ALHGITAPEF YDKKLYATLS VKLKELGYLT DNQGCSDIKR 790 800 IKERANLLLR SSVKQTIVDS VHAEHIA

References

[1]

"Complete sequence of Shewanella halifaxensis HAW-EB4." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Kim E., Zhao J.-S., Richardson P. Submitted (JAN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HAW-EB4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000931 Genomic DNA. Translation: ABZ74847.1.
RefSeq	YP_001672506.1. NC_010334.1.
3D structure databases
ProteinModelPortal	B0TNU4.
ModBase	Search...
Protein-protein interaction databases
STRING	B0TNU4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5906541.
GenomeReviews	Gene locus Shal_0271 in contig CP000931_GR.
KEGG	shl:Shal_0271.
PATRIC	23502962. VBISheHal24697_0289.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG296590.
OMA	WNKLYQG.
ProtClustDB	PRK04974.
Enzyme and pathway databases
BioCyc	SHAL458817:SHAL_0271-MONOMER.
Family and domain databases
HAMAP	MF_00393. Glyc3P_acyltrans. [Tree]
InterPro	IPR002123. Acyltransferase. IPR022284. G3P_O-AcylTrfase. [Graphical view]
KO	K00631.
Pfam	PF01553. Acyltransferase. 1 hit. [Graphical view]
PIRSF	PIRSF000437. GPAT_DHAPAT. 1 hit.
SMART	SM00563. PlsC. 1 hit. [Graphical view]
TIGRFAMs	TIGR03703. PlsB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PLSB_SHEHH

Accession

Primary (citable) accession number: B0TNU4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	April 8, 2008
Last modified:	January 25, 2012
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program