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B0TL21

- FADJ_SHEHH

UniProt

B0TL21 - FADJ_SHEHH

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Protein

Fatty acid oxidation complex subunit alpha

Gene

fadJ

Organism
Shewanella halifaxensis (strain HAW-EB4)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the formation of a hydroxyacyl-CoA by addition of water on enoyl-CoA. Also exhibits 3-hydroxyacyl-CoA epimerase and 3-hydroxyacyl-CoA dehydrogenase activities.UniRule annotation

Catalytic activityi

(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O.UniRule annotation
(S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH.UniRule annotation
(S)-3-hydroxybutanoyl-CoA = (R)-3-hydroxybutanoyl-CoA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei118 – 1181Important for catalytic activityUniRule annotation
Sitei140 – 1401Important for catalytic activityUniRule annotation

GO - Molecular functioni

  1. 3-hydroxyacyl-CoA dehydrogenase activity Source: UniProtKB-HAMAP
  2. 3-hydroxybutyryl-CoA epimerase activity Source: UniProtKB-HAMAP
  3. enoyl-CoA hydratase activity Source: UniProtKB-HAMAP
  4. NAD binding Source: InterPro

GO - Biological processi

  1. fatty acid beta-oxidation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Lyase, Oxidoreductase

Keywords - Biological processi

Fatty acid metabolism, Lipid degradation, Lipid metabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciSHAL458817:GH1X-2766-MONOMER.
UniPathwayiUPA00659.

Names & Taxonomyi

Protein namesi
Recommended name:
Fatty acid oxidation complex subunit alphaUniRule annotation
Including the following 2 domains:
Enoyl-CoA hydratase/3-hydroxybutyryl-CoA epimeraseUniRule annotation (EC:4.2.1.17UniRule annotation, EC:5.1.2.3UniRule annotation)
3-hydroxyacyl-CoA dehydrogenaseUniRule annotation (EC:1.1.1.35UniRule annotation)
Gene namesi
Name:fadJUniRule annotation
Ordered Locus Names:Shal_2670
OrganismiShewanella halifaxensis (strain HAW-EB4)
Taxonomic identifieri458817 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
ProteomesiUP000001317: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 708708Fatty acid oxidation complex subunit alphaPRO_1000088063Add
BLAST

Interactioni

Subunit structurei

Heterotetramer of two alpha chains (FadJ) and two beta chains (FadI).UniRule annotation

Protein-protein interaction databases

STRINGi458817.Shal_2670.

Structurei

3D structure databases

ProteinModelPortaliB0TL21.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 190190Enoyl-CoA hydrataseUniRule annotationAdd
BLAST
Regioni310 – 7083993-hydroxyacyl-CoA dehydrogenaseUniRule annotationAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.UniRule annotation
In the central section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1250.
HOGENOMiHOG000261346.
KOiK01782.
OMAiPFRYMDT.
OrthoDBiEOG6M9F0M.

Family and domain databases

Gene3Di1.10.1040.10. 2 hits.
3.40.50.720. 1 hit.
3.90.226.10. 1 hit.
HAMAPiMF_01617. FadJ.
InterProiIPR006176. 3-OHacyl-CoA_DH_NAD-bd.
IPR006108. 3HC_DH_C.
IPR008927. 6-PGluconate_DH_C-like.
IPR029045. ClpP/crotonase-like_dom.
IPR001753. Crotonase_core_superfam.
IPR013328. DH_multihelical.
IPR012802. FadJ.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00725. 3HCDH. 2 hits.
PF02737. 3HCDH_N. 1 hit.
PF00378. ECH. 1 hit.
[Graphical view]
SUPFAMiSSF48179. SSF48179. 2 hits.
SSF52096. SSF52096. 1 hit.
TIGRFAMsiTIGR02440. FadJ. 1 hit.

Sequencei

Sequence statusi: Complete.

B0TL21-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDMEKTFNLT RRDDGIAILT MDVPGETMNT LRSEFGPEIS DVLAEIKADT
60 70 80 90 100
SIKGLVLVSG KKDSFVAGAD ISMLDACETA TDAKQLSQQG HVVFNELESL
110 120 130 140 150
PIPVVAAING ACLGGGLELA LACHKRVCSL NPKTMMGVPE VQLGLLPGGG
160 170 180 190 200
GTQRLPRLVG VTTALDMMLT GKQLRPKQAL KMGLVDDAVP ESILLRTAVE
210 220 230 240 250
MALAGKRPAK KKHQSFFNKV LEGTSAGRNI IFDQAGKQVA KKTQGNYPAP
260 270 280 290 300
AKIIDCVRQG MTKGMAKGLE VEASHFADLV MSKESGAMRS VFFATTEMKK
310 320 330 340 350
ETGAGDVEPQ KVNKVMVLGG GLMGGGIASV TTTKAKIPAR VKDISETGLS
360 370 380 390 400
NALAYAYKLL DKGVKRRHMT PAVRDNLMAL MTTTTEYKGI KDADIVVEAV
410 420 430 440 450
FEDLALKHQM VKDVERECGE HTIFASNTSS LPIGQIAEAA SRPENVIGLH
460 470 480 490 500
YFSPVEKMPL VEVIAHEKTS AETIATTVAF AKKQGKTPIV VQDGAGFYVN
510 520 530 540 550
RILALYMNEA AQLLLEGQSV EHLDKALIKF GFPVGPMTLL DEVGIDVGAK
560 570 580 590 600
ISPILEKELG ERFKAPTAFD KLLADDRKGR KNGKGFYLYG GKKKAKEVDQ
610 620 630 640 650
TVYSVLGLKP GVDTEATEVA QRCVVQMLNE AVRCLEESII ACPRDGDIGA
660 670 680 690 700
IFGIGFPPFL GGPFHYIDTL GAANLVKILE NYQSRYGSRF EPAAKLKQMA

EEGTRFFS
Length:708
Mass (Da):76,230
Last modified:April 8, 2008 - v1
Checksum:iA0C731D06441039D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000931 Genomic DNA. Translation: ABZ77223.1.
RefSeqiYP_001674882.1. NC_010334.1.

Genome annotation databases

EnsemblBacteriaiABZ77223; ABZ77223; Shal_2670.
GeneIDi5902951.
KEGGishl:Shal_2670.
PATRICi23508199. VBISheHal24697_2810.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000931 Genomic DNA. Translation: ABZ77223.1 .
RefSeqi YP_001674882.1. NC_010334.1.

3D structure databases

ProteinModelPortali B0TL21.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 458817.Shal_2670.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABZ77223 ; ABZ77223 ; Shal_2670 .
GeneIDi 5902951.
KEGGi shl:Shal_2670.
PATRICi 23508199. VBISheHal24697_2810.

Phylogenomic databases

eggNOGi COG1250.
HOGENOMi HOG000261346.
KOi K01782.
OMAi PFRYMDT.
OrthoDBi EOG6M9F0M.

Enzyme and pathway databases

UniPathwayi UPA00659 .
BioCyci SHAL458817:GH1X-2766-MONOMER.

Family and domain databases

Gene3Di 1.10.1040.10. 2 hits.
3.40.50.720. 1 hit.
3.90.226.10. 1 hit.
HAMAPi MF_01617. FadJ.
InterProi IPR006176. 3-OHacyl-CoA_DH_NAD-bd.
IPR006108. 3HC_DH_C.
IPR008927. 6-PGluconate_DH_C-like.
IPR029045. ClpP/crotonase-like_dom.
IPR001753. Crotonase_core_superfam.
IPR013328. DH_multihelical.
IPR012802. FadJ.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
Pfami PF00725. 3HCDH. 2 hits.
PF02737. 3HCDH_N. 1 hit.
PF00378. ECH. 1 hit.
[Graphical view ]
SUPFAMi SSF48179. SSF48179. 2 hits.
SSF52096. SSF52096. 1 hit.
TIGRFAMsi TIGR02440. FadJ. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HAW-EB4.

Entry informationi

Entry nameiFADJ_SHEHH
AccessioniPrimary (citable) accession number: B0TL21
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: April 8, 2008
Last modified: October 29, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3