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B0TL21

- FADJ_SHEHH

UniProt

B0TL21 - FADJ_SHEHH

Protein

Fatty acid oxidation complex subunit alpha

Gene

fadJ

Organism
Shewanella halifaxensis (strain HAW-EB4)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 50 (01 Oct 2014)
      Sequence version 1 (08 Apr 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the formation of a hydroxyacyl-CoA by addition of water on enoyl-CoA. Also exhibits 3-hydroxyacyl-CoA epimerase and 3-hydroxyacyl-CoA dehydrogenase activities.UniRule annotation

    Catalytic activityi

    (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O.UniRule annotation
    (S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH.UniRule annotation
    (S)-3-hydroxybutanoyl-CoA = (R)-3-hydroxybutanoyl-CoA.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei118 – 1181Important for catalytic activityUniRule annotation
    Sitei140 – 1401Important for catalytic activityUniRule annotation

    GO - Molecular functioni

    1. 3-hydroxyacyl-CoA dehydrogenase activity Source: UniProtKB-HAMAP
    2. 3-hydroxybutyryl-CoA epimerase activity Source: UniProtKB-HAMAP
    3. enoyl-CoA hydratase activity Source: UniProtKB-HAMAP
    4. NAD binding Source: InterPro

    GO - Biological processi

    1. fatty acid beta-oxidation Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Isomerase, Lyase, Oxidoreductase

    Keywords - Biological processi

    Fatty acid metabolism, Lipid degradation, Lipid metabolism

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    BioCyciSHAL458817:GH1X-2766-MONOMER.
    UniPathwayiUPA00659.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Fatty acid oxidation complex subunit alphaUniRule annotation
    Including the following 2 domains:
    Enoyl-CoA hydratase/3-hydroxybutyryl-CoA epimeraseUniRule annotation (EC:4.2.1.17UniRule annotation, EC:5.1.2.3UniRule annotation)
    3-hydroxyacyl-CoA dehydrogenaseUniRule annotation (EC:1.1.1.35UniRule annotation)
    Gene namesi
    Name:fadJUniRule annotation
    Ordered Locus Names:Shal_2670
    OrganismiShewanella halifaxensis (strain HAW-EB4)
    Taxonomic identifieri458817 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
    ProteomesiUP000001317: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 708708Fatty acid oxidation complex subunit alphaPRO_1000088063Add
    BLAST

    Interactioni

    Subunit structurei

    Heterotetramer of two alpha chains (FadJ) and two beta chains (FadI).UniRule annotation

    Protein-protein interaction databases

    STRINGi458817.Shal_2670.

    Structurei

    3D structure databases

    ProteinModelPortaliB0TL21.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 190190Enoyl-CoA hydrataseUniRule annotationAdd
    BLAST
    Regioni310 – 7083993-hydroxyacyl-CoA dehydrogenaseUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.UniRule annotation
    In the central section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1250.
    HOGENOMiHOG000261346.
    KOiK01782.
    OMAiPFRYMDT.
    OrthoDBiEOG6M9F0M.

    Family and domain databases

    Gene3Di1.10.1040.10. 2 hits.
    3.40.50.720. 1 hit.
    3.90.226.10. 1 hit.
    HAMAPiMF_01617. FadJ.
    InterProiIPR006176. 3-OHacyl-CoA_DH_NAD-bd.
    IPR006108. 3HC_DH_C.
    IPR008927. 6-PGluconate_DH_C-like.
    IPR029045. ClpP/crotonase-like_dom.
    IPR001753. Crotonase_core_superfam.
    IPR013328. DH_multihelical.
    IPR012802. FadJ.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PfamiPF00725. 3HCDH. 2 hits.
    PF02737. 3HCDH_N. 1 hit.
    PF00378. ECH. 1 hit.
    [Graphical view]
    SUPFAMiSSF48179. SSF48179. 2 hits.
    SSF52096. SSF52096. 1 hit.
    TIGRFAMsiTIGR02440. FadJ. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    B0TL21-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDMEKTFNLT RRDDGIAILT MDVPGETMNT LRSEFGPEIS DVLAEIKADT    50
    SIKGLVLVSG KKDSFVAGAD ISMLDACETA TDAKQLSQQG HVVFNELESL 100
    PIPVVAAING ACLGGGLELA LACHKRVCSL NPKTMMGVPE VQLGLLPGGG 150
    GTQRLPRLVG VTTALDMMLT GKQLRPKQAL KMGLVDDAVP ESILLRTAVE 200
    MALAGKRPAK KKHQSFFNKV LEGTSAGRNI IFDQAGKQVA KKTQGNYPAP 250
    AKIIDCVRQG MTKGMAKGLE VEASHFADLV MSKESGAMRS VFFATTEMKK 300
    ETGAGDVEPQ KVNKVMVLGG GLMGGGIASV TTTKAKIPAR VKDISETGLS 350
    NALAYAYKLL DKGVKRRHMT PAVRDNLMAL MTTTTEYKGI KDADIVVEAV 400
    FEDLALKHQM VKDVERECGE HTIFASNTSS LPIGQIAEAA SRPENVIGLH 450
    YFSPVEKMPL VEVIAHEKTS AETIATTVAF AKKQGKTPIV VQDGAGFYVN 500
    RILALYMNEA AQLLLEGQSV EHLDKALIKF GFPVGPMTLL DEVGIDVGAK 550
    ISPILEKELG ERFKAPTAFD KLLADDRKGR KNGKGFYLYG GKKKAKEVDQ 600
    TVYSVLGLKP GVDTEATEVA QRCVVQMLNE AVRCLEESII ACPRDGDIGA 650
    IFGIGFPPFL GGPFHYIDTL GAANLVKILE NYQSRYGSRF EPAAKLKQMA 700
    EEGTRFFS 708
    Length:708
    Mass (Da):76,230
    Last modified:April 8, 2008 - v1
    Checksum:iA0C731D06441039D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000931 Genomic DNA. Translation: ABZ77223.1.
    RefSeqiWP_012277751.1. NC_010334.1.
    YP_001674882.1. NC_010334.1.

    Genome annotation databases

    EnsemblBacteriaiABZ77223; ABZ77223; Shal_2670.
    GeneIDi5902951.
    KEGGishl:Shal_2670.
    PATRICi23508199. VBISheHal24697_2810.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000931 Genomic DNA. Translation: ABZ77223.1 .
    RefSeqi WP_012277751.1. NC_010334.1.
    YP_001674882.1. NC_010334.1.

    3D structure databases

    ProteinModelPortali B0TL21.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 458817.Shal_2670.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABZ77223 ; ABZ77223 ; Shal_2670 .
    GeneIDi 5902951.
    KEGGi shl:Shal_2670.
    PATRICi 23508199. VBISheHal24697_2810.

    Phylogenomic databases

    eggNOGi COG1250.
    HOGENOMi HOG000261346.
    KOi K01782.
    OMAi PFRYMDT.
    OrthoDBi EOG6M9F0M.

    Enzyme and pathway databases

    UniPathwayi UPA00659 .
    BioCyci SHAL458817:GH1X-2766-MONOMER.

    Family and domain databases

    Gene3Di 1.10.1040.10. 2 hits.
    3.40.50.720. 1 hit.
    3.90.226.10. 1 hit.
    HAMAPi MF_01617. FadJ.
    InterProi IPR006176. 3-OHacyl-CoA_DH_NAD-bd.
    IPR006108. 3HC_DH_C.
    IPR008927. 6-PGluconate_DH_C-like.
    IPR029045. ClpP/crotonase-like_dom.
    IPR001753. Crotonase_core_superfam.
    IPR013328. DH_multihelical.
    IPR012802. FadJ.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    Pfami PF00725. 3HCDH. 2 hits.
    PF02737. 3HCDH_N. 1 hit.
    PF00378. ECH. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48179. SSF48179. 2 hits.
    SSF52096. SSF52096. 1 hit.
    TIGRFAMsi TIGR02440. FadJ. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: HAW-EB4.

    Entry informationi

    Entry nameiFADJ_SHEHH
    AccessioniPrimary (citable) accession number: B0TL21
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 20, 2008
    Last sequence update: April 8, 2008
    Last modified: October 1, 2014
    This is version 50 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3