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B0TJN8 (BIOB_SHEHH) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 43. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:Shal_2477
OrganismShewanella halifaxensis (strain HAW-EB4) [Complete proteome] [HAMAP]
Taxonomic identifier458817 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella

Protein attributes

Sequence length350 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 350350Biotin synthase HAMAP-Rule MF_01694
PRO_0000381619

Sites

Metal binding561Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding601Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding631Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1001Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1311Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1911Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2631Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
B0TJN8 [UniParc].

Last modified April 8, 2008. Version 1.
Checksum: A1524D5EFAB96EE5

FASTA35038,875
        10         20         30         40         50         60 
MSEVQLRNNW KREEIESLFA LPMNDLLFQA HSIHRQEFDP NEVQISRLLS IKTGACPEDC 

        70         80         90        100        110        120 
KYCPQSARYD TGLEKERLLA METVLTEARS AKAAGASRFC MGAAWRNPKE RDMPYLKTMV 

       130        140        150        160        170        180 
EEVKALGMET CMTLGMLSAE QANTLADAGL DYYNHNLDTS PEYYGDVITT RTYQSRLDTL 

       190        200        210        220        230        240 
SNVRASGMKV CSGGIVGMGE KATDRAGLIQ QLANLDQHPD SVPINMLVKV EGTPFEKLDD 

       250        260        270        280        290        300 
LDPLEFVRTI AVARITMPKS RVRLSAGREN MSDELQAMCF FAGANSIFYG CKLLTTPNPE 

       310        320        330        340        350 
ENDDMGLFRR LGLHPEQGVA STKEQDEAML AKAAAQQDKK VSAFYDAGAL 

« Hide

References

[1]"Complete sequence of Shewanella halifaxensis HAW-EB4."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. expand/collapse author list , Kyrpides N., Kim E., Zhao J.-S., Richardson P.
Submitted (JAN-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: HAW-EB4.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000931 Genomic DNA. Translation: ABZ77034.1.
RefSeqYP_001674693.1. NC_010334.1.

3D structure databases

ProteinModelPortalB0TJN8.
SMRB0TJN8. Positions 7-317.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING458817.Shal_2477.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABZ77034; ABZ77034; Shal_2477.
GeneID5905856.
KEGGshl:Shal_2477.
PATRIC23507769. VBISheHal24697_2599.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239957.
KOK01012.
OMAADRFCMG.
OrthoDBEOG622PMP.
ProtClustDBCLSK2304484.

Enzyme and pathway databases

BioCycSHAL458817:GH1X-2569-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_SHEHH
AccessionPrimary (citable) accession number: B0TJN8
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: April 8, 2008
Last modified: February 19, 2014
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways