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B0THS2

- PNP_HELMI

UniProt

B0THS2 - PNP_HELMI

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Protein

Polyribonucleotide nucleotidyltransferase

Gene
pnp, Helmi_22300, HM1_2322
Organism
Heliobacterium modesticaldum (strain ATCC 51547 / Ice1)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction By similarity.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi482 – 4821Magnesium By similarity
Metal bindingi488 – 4881Magnesium By similarity

GO - Molecular functioni

  1. 3'-5'-exoribonuclease activity Source: InterPro
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
  4. RNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. mRNA catabolic process Source: UniProtKB-HAMAP
  2. RNA processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciHMOD498761:GI46-2314-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferase (EC:2.7.7.8)
Alternative name(s):
Polynucleotide phosphorylase
Short name:
PNPase
Gene namesi
Name:pnp
Ordered Locus Names:Helmi_22300
ORF Names:HM1_2322
OrganismiHeliobacterium modesticaldum (strain ATCC 51547 / Ice1)
Taxonomic identifieri498761 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesHeliobacteriaceaeHeliobacterium
ProteomesiUP000008550: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 709709Polyribonucleotide nucleotidyltransferaseUniRule annotationPRO_0000381904Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi498761.HM1_2322.

Structurei

3D structure databases

ProteinModelPortaliB0THS2.
SMRiB0THS2. Positions 613-687.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini549 – 60860KHAdd
BLAST
Domaini618 – 68669S1 motifAdd
BLAST

Sequence similaritiesi

Contains 1 KH domain.
Contains 1 S1 motif domain.

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218327.
KOiK00962.
OMAiISMEDTH.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B0THS2-1 [UniParc]FASTAAdd to Basket

« Hide

MQVFSMEIAG RTLTIETGRV AKQAGGSVLA RYGDTVVLVT ATGSKEPRPG    50
IDFFPLTVDY EERLYAVGKI PGGFIKREGR PTEKAILSAR LIDRPIRPLF 100
PKGFRNDVQV VATVMSVDQD CPPDIVGMIG ASCALSLSDI PFEGPIGGVL 150
VGRVDGKLLI NPTMEQAEKS DMHLVVAGTK DAVMMVEAGA NEVPEEDMIE 200
AIMFGHQEIQ RIVAFQEEMV AVAGKPKREV PLKQINPELE QAVREYVGDK 250
LRNAIQNPDK LSREADIQAV MKETVEALLP HFPEEEKDIR TVLDTMEKEI 300
VRKLITVDKQ RPDGRKMDEI RPISVEVGIL PRTHGSGLFT RGQTQVLNVC 350
TLGTIADLQI LDGLGVEESK RYMHHYNFPP YSVGETRPMR GPGRREIGHG 400
ALAERALLPV IPSEDEFPYT IRLVSEAVES NGSTSMASVC GSTLSLMNAG 450
VPIKKPVAGI AMGLIKEGEH FSILSDIQGM EDHLGDMDFK VAGTADGVTA 500
LQMDIKIKGV NREILTQALQ QARKGRLFIL DKMLAVIDKP AAEMSPYAPR 550
IITMSIDPDK IREVIGPGGK VINKIIAETG VKIDIEDDGR IFIAATDTEA 600
ANKAVRIIES ITADVEVGKV YTGKVTRIMN FGAFVEVLPG KEGLIHISQL 650
AEERVAKVED VVKIGDEVTV KVVEIDKQGR INLSRKEVLK ANKPAVTGGA 700
RPDEMRRRF 709
Length:709
Mass (Da):77,495
Last modified:April 8, 2008 - v1
Checksum:iCA6AD8C3C1104025
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000930 Genomic DNA. Translation: ABZ84855.1.
RefSeqiYP_001680866.1. NC_010337.2.

Genome annotation databases

EnsemblBacteriaiABZ84855; ABZ84855; HM1_2322.
GeneIDi5909266.
KEGGihmo:HM1_2322.
PATRICi22108701. VBIHelMod36755_2088.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000930 Genomic DNA. Translation: ABZ84855.1 .
RefSeqi YP_001680866.1. NC_010337.2.

3D structure databases

ProteinModelPortali B0THS2.
SMRi B0THS2. Positions 613-687.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 498761.HM1_2322.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABZ84855 ; ABZ84855 ; HM1_2322 .
GeneIDi 5909266.
KEGGi hmo:HM1_2322.
PATRICi 22108701. VBIHelMod36755_2088.

Phylogenomic databases

eggNOGi COG1185.
HOGENOMi HOG000218327.
KOi K00962.
OMAi ISMEDTH.
OrthoDBi EOG6WT8CC.

Enzyme and pathway databases

BioCyci HMOD498761:GI46-2314-MONOMER.

Family and domain databases

Gene3Di 1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPi MF_01595. PNPase.
InterProi IPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view ]
PANTHERi PTHR11252. PTHR11252. 1 hit.
Pfami PF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view ]
PIRSFi PIRSF005499. PNPase. 1 hit.
SMARTi SM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view ]
SUPFAMi SSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
PROSITEi PS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome of Heliobacterium modesticaldum, a phototrophic representative of the Firmicutes containing the simplest photosynthetic apparatus."
    Sattley W.M., Madigan M.T., Swingley W.D., Cheung P.C., Clocksin K.M., Conrad A.L., Dejesa L.C., Honchak B.M., Jung D.O., Karbach L.E., Kurdoglu A., Lahiri S., Mastrian S.D., Page L.E., Taylor H.L., Wang Z.T., Raymond J., Chen M., Blankenship R.E., Touchman J.W.
    J. Bacteriol. 190:4687-4696(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 51547 / Ice1.

Entry informationi

Entry nameiPNP_HELMI
AccessioniPrimary (citable) accession number: B0THS2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 1, 2009
Last sequence update: April 8, 2008
Last modified: May 14, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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