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Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Heliobacterium modesticaldum (strain ATCC 51547 / Ice1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi482MagnesiumUniRule annotation1
Metal bindingi488MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Ordered Locus Names:Helmi_22300
ORF Names:HM1_2322
OrganismiHeliobacterium modesticaldum (strain ATCC 51547 / Ice1)
Taxonomic identifieri498761 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesHeliobacteriaceaeHeliobacterium
Proteomesi
  • UP000008550 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003819041 – 709Polyribonucleotide nucleotidyltransferaseAdd BLAST709

Interactioni

Protein-protein interaction databases

STRINGi498761.HM1_2322.

Structurei

3D structure databases

ProteinModelPortaliB0THS2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini549 – 608KHUniRule annotationAdd BLAST60
Domaini618 – 686S1 motifUniRule annotationAdd BLAST69

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
Contains 1 KH domain.UniRule annotation
Contains 1 S1 motif domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C62. Bacteria.
COG1185. LUCA.
HOGENOMiHOG000218327.
KOiK00962.
OMAiRFMFHYN.
OrthoDBiPOG091H00M0.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase. 1 hit.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
IPR003029. S1_domain.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B0THS2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQVFSMEIAG RTLTIETGRV AKQAGGSVLA RYGDTVVLVT ATGSKEPRPG
60 70 80 90 100
IDFFPLTVDY EERLYAVGKI PGGFIKREGR PTEKAILSAR LIDRPIRPLF
110 120 130 140 150
PKGFRNDVQV VATVMSVDQD CPPDIVGMIG ASCALSLSDI PFEGPIGGVL
160 170 180 190 200
VGRVDGKLLI NPTMEQAEKS DMHLVVAGTK DAVMMVEAGA NEVPEEDMIE
210 220 230 240 250
AIMFGHQEIQ RIVAFQEEMV AVAGKPKREV PLKQINPELE QAVREYVGDK
260 270 280 290 300
LRNAIQNPDK LSREADIQAV MKETVEALLP HFPEEEKDIR TVLDTMEKEI
310 320 330 340 350
VRKLITVDKQ RPDGRKMDEI RPISVEVGIL PRTHGSGLFT RGQTQVLNVC
360 370 380 390 400
TLGTIADLQI LDGLGVEESK RYMHHYNFPP YSVGETRPMR GPGRREIGHG
410 420 430 440 450
ALAERALLPV IPSEDEFPYT IRLVSEAVES NGSTSMASVC GSTLSLMNAG
460 470 480 490 500
VPIKKPVAGI AMGLIKEGEH FSILSDIQGM EDHLGDMDFK VAGTADGVTA
510 520 530 540 550
LQMDIKIKGV NREILTQALQ QARKGRLFIL DKMLAVIDKP AAEMSPYAPR
560 570 580 590 600
IITMSIDPDK IREVIGPGGK VINKIIAETG VKIDIEDDGR IFIAATDTEA
610 620 630 640 650
ANKAVRIIES ITADVEVGKV YTGKVTRIMN FGAFVEVLPG KEGLIHISQL
660 670 680 690 700
AEERVAKVED VVKIGDEVTV KVVEIDKQGR INLSRKEVLK ANKPAVTGGA

RPDEMRRRF
Length:709
Mass (Da):77,495
Last modified:April 8, 2008 - v1
Checksum:iCA6AD8C3C1104025
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000930 Genomic DNA. Translation: ABZ84855.1.

Genome annotation databases

EnsemblBacteriaiABZ84855; ABZ84855; HM1_2322.
KEGGihmo:HM1_2322.
PATRICi22108701. VBIHelMod36755_2088.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000930 Genomic DNA. Translation: ABZ84855.1.

3D structure databases

ProteinModelPortaliB0THS2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi498761.HM1_2322.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABZ84855; ABZ84855; HM1_2322.
KEGGihmo:HM1_2322.
PATRICi22108701. VBIHelMod36755_2088.

Phylogenomic databases

eggNOGiENOG4105C62. Bacteria.
COG1185. LUCA.
HOGENOMiHOG000218327.
KOiK00962.
OMAiRFMFHYN.
OrthoDBiPOG091H00M0.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase. 1 hit.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
IPR003029. S1_domain.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPNP_HELMI
AccessioniPrimary (citable) accession number: B0THS2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 1, 2009
Last sequence update: April 8, 2008
Last modified: November 2, 2016
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.