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B0THS2

- PNP_HELMI

UniProt

B0THS2 - PNP_HELMI

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Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Heliobacterium modesticaldum (strain ATCC 51547 / Ice1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Magnesium.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi482 – 4821MagnesiumUniRule annotation
Metal bindingi488 – 4881MagnesiumUniRule annotation

GO - Molecular functioni

  1. 3'-5'-exoribonuclease activity Source: InterPro
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
  4. RNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. mRNA catabolic process Source: UniProtKB-HAMAP
  2. RNA processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciHMOD498761:GI46-2314-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Ordered Locus Names:Helmi_22300
ORF Names:HM1_2322
OrganismiHeliobacterium modesticaldum (strain ATCC 51547 / Ice1)
Taxonomic identifieri498761 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesHeliobacteriaceaeHeliobacterium
ProteomesiUP000008550: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 709709Polyribonucleotide nucleotidyltransferasePRO_0000381904Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi498761.HM1_2322.

Structurei

3D structure databases

ProteinModelPortaliB0THS2.
SMRiB0THS2. Positions 613-687.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini549 – 60860KHUniRule annotationAdd
BLAST
Domaini618 – 68669S1 motifUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
Contains 1 KH domain.UniRule annotation
Contains 1 S1 motif domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218327.
KOiK00962.
OMAiISMEDTH.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B0THS2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQVFSMEIAG RTLTIETGRV AKQAGGSVLA RYGDTVVLVT ATGSKEPRPG
60 70 80 90 100
IDFFPLTVDY EERLYAVGKI PGGFIKREGR PTEKAILSAR LIDRPIRPLF
110 120 130 140 150
PKGFRNDVQV VATVMSVDQD CPPDIVGMIG ASCALSLSDI PFEGPIGGVL
160 170 180 190 200
VGRVDGKLLI NPTMEQAEKS DMHLVVAGTK DAVMMVEAGA NEVPEEDMIE
210 220 230 240 250
AIMFGHQEIQ RIVAFQEEMV AVAGKPKREV PLKQINPELE QAVREYVGDK
260 270 280 290 300
LRNAIQNPDK LSREADIQAV MKETVEALLP HFPEEEKDIR TVLDTMEKEI
310 320 330 340 350
VRKLITVDKQ RPDGRKMDEI RPISVEVGIL PRTHGSGLFT RGQTQVLNVC
360 370 380 390 400
TLGTIADLQI LDGLGVEESK RYMHHYNFPP YSVGETRPMR GPGRREIGHG
410 420 430 440 450
ALAERALLPV IPSEDEFPYT IRLVSEAVES NGSTSMASVC GSTLSLMNAG
460 470 480 490 500
VPIKKPVAGI AMGLIKEGEH FSILSDIQGM EDHLGDMDFK VAGTADGVTA
510 520 530 540 550
LQMDIKIKGV NREILTQALQ QARKGRLFIL DKMLAVIDKP AAEMSPYAPR
560 570 580 590 600
IITMSIDPDK IREVIGPGGK VINKIIAETG VKIDIEDDGR IFIAATDTEA
610 620 630 640 650
ANKAVRIIES ITADVEVGKV YTGKVTRIMN FGAFVEVLPG KEGLIHISQL
660 670 680 690 700
AEERVAKVED VVKIGDEVTV KVVEIDKQGR INLSRKEVLK ANKPAVTGGA

RPDEMRRRF
Length:709
Mass (Da):77,495
Last modified:April 8, 2008 - v1
Checksum:iCA6AD8C3C1104025
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000930 Genomic DNA. Translation: ABZ84855.1.
RefSeqiWP_012283353.1. NC_010337.2.
YP_001680866.1. NC_010337.2.

Genome annotation databases

EnsemblBacteriaiABZ84855; ABZ84855; HM1_2322.
GeneIDi5909266.
KEGGihmo:HM1_2322.
PATRICi22108701. VBIHelMod36755_2088.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000930 Genomic DNA. Translation: ABZ84855.1 .
RefSeqi WP_012283353.1. NC_010337.2.
YP_001680866.1. NC_010337.2.

3D structure databases

ProteinModelPortali B0THS2.
SMRi B0THS2. Positions 613-687.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 498761.HM1_2322.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABZ84855 ; ABZ84855 ; HM1_2322 .
GeneIDi 5909266.
KEGGi hmo:HM1_2322.
PATRICi 22108701. VBIHelMod36755_2088.

Phylogenomic databases

eggNOGi COG1185.
HOGENOMi HOG000218327.
KOi K00962.
OMAi ISMEDTH.
OrthoDBi EOG6WT8CC.

Enzyme and pathway databases

BioCyci HMOD498761:GI46-2314-MONOMER.

Family and domain databases

Gene3Di 1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPi MF_01595. PNPase.
InterProi IPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view ]
PANTHERi PTHR11252. PTHR11252. 1 hit.
Pfami PF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view ]
PIRSFi PIRSF005499. PNPase. 1 hit.
SMARTi SM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view ]
SUPFAMi SSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
PROSITEi PS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome of Heliobacterium modesticaldum, a phototrophic representative of the Firmicutes containing the simplest photosynthetic apparatus."
    Sattley W.M., Madigan M.T., Swingley W.D., Cheung P.C., Clocksin K.M., Conrad A.L., Dejesa L.C., Honchak B.M., Jung D.O., Karbach L.E., Kurdoglu A., Lahiri S., Mastrian S.D., Page L.E., Taylor H.L., Wang Z.T., Raymond J., Chen M., Blankenship R.E., Touchman J.W.
    J. Bacteriol. 190:4687-4696(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 51547 / Ice1.

Entry informationi

Entry nameiPNP_HELMI
AccessioniPrimary (citable) accession number: B0THS2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 1, 2009
Last sequence update: April 8, 2008
Last modified: October 29, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3