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Protein

Glutamyl-tRNA(Gln) amidotransferase subunit A

Gene

gatA

Organism
Heliobacterium modesticaldum (strain ATCC 51547 / Ice1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).UniRule annotation

Catalytic activityi

ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei79Charge relay systemUniRule annotation1
Active sitei154Charge relay systemUniRule annotation1
Active sitei178Acyl-ester intermediateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciHMOD498761:G1GB0-2782-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamyl-tRNA(Gln) amidotransferase subunit AUniRule annotation (EC:6.3.5.7UniRule annotation)
Short name:
Glu-ADT subunit AUniRule annotation
Gene namesi
Name:gatAUniRule annotation
Ordered Locus Names:Helmi_29070
ORF Names:HM1_3025
OrganismiHeliobacterium modesticaldum (strain ATCC 51547 / Ice1)
Taxonomic identifieri498761 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesHeliobacteriaceaeHeliobacterium
Proteomesi
  • UP000008550 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000951371 – 487Glutamyl-tRNA(Gln) amidotransferase subunit AAdd BLAST487

Interactioni

Subunit structurei

Heterotrimer of A, B and C subunits.UniRule annotation

Protein-protein interaction databases

STRINGi498761.HM1_3025.

Structurei

3D structure databases

ProteinModelPortaliB0TDK7.
SMRiB0TDK7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the amidase family. GatA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C3P. Bacteria.
COG0154. LUCA.
HOGENOMiHOG000116699.
KOiK02433.
OMAiPITQNVE.
OrthoDBiPOG091H00O9.

Family and domain databases

Gene3Di3.90.1300.10. 1 hit.
HAMAPiMF_00120. GatA. 1 hit.
InterProiView protein in InterPro
IPR000120. Amidase.
IPR020556. Amidase_CS.
IPR023631. Amidase_dom.
IPR036928. AS_sf.
IPR004412. GatA.
PANTHERiPTHR11895. PTHR11895. 1 hit.
PfamiView protein in Pfam
PF01425. Amidase. 1 hit.
SUPFAMiSSF75304. SSF75304. 1 hit.
TIGRFAMsiTIGR00132. gatA. 1 hit.
PROSITEiView protein in PROSITE
PS00571. AMIDASES. 1 hit.

Sequencei

Sequence statusi: Complete.

B0TDK7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLYTKTAHE LHDLLVRKEV SATEIVKTQA DRMQALEPKI RAFVTLTVDK
60 70 80 90 100
ALEQAARVDA KIAAGEAIGP LEGIPMAIKD NMCTDGVRTT CSSKILNNFV
110 120 130 140 150
PPYDATVVTK LKEAGAVMMG KTNLDEFAMG SSTENSGFFA TCNPWDIERV
160 170 180 190 200
PGGSSGGSAA SVAAGQAVFS LGSDTGGSIR QPAAFCGVVG LKPTYGAVSR
210 220 230 240 250
FGLIAFASSL DQIGPFTRDV RDCAHVMNAI AGHDAKDSTS APVDYPDYTS
260 270 280 290 300
LLGKPIKGWR VGLPKEYFGD GMDPEVKEVI EKAVKTIEDL GAEVAECSLP
310 320 330 340 350
HTEYALPVYY LIAPAEASSN LARYDGVRYG YRSESADDLI TMFKKTRAEG
360 370 380 390 400
FGDEVKRRIM LGTYALSSGY YDAYYLKALK VRTLIKNDFD QAFEKFDVIL
410 420 430 440 450
SPTTPTVAFK FGDRTDNPLQ MYLSDIYTLS VNLAGIPGLS INAGFAKGMP
460 470 480
VGLQIIGKPF DEARLLQIAY AYEEATGCHS KMPDLGV
Length:487
Mass (Da):52,524
Last modified:April 8, 2008 - v1
Checksum:i63A88959D0F5E8E1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000930 Genomic DNA. Translation: ABZ85532.1.
RefSeqiWP_012284007.1. NC_010337.2.

Genome annotation databases

EnsemblBacteriaiABZ85532; ABZ85532; HM1_3025.
KEGGihmo:HM1_3025.

Similar proteinsi

Entry informationi

Entry nameiGATA_HELMI
AccessioniPrimary (citable) accession number: B0TDK7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: April 8, 2008
Last modified: February 28, 2018
This is version 65 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome