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Protein

Glutamyl-tRNA(Gln) amidotransferase subunit A

Gene

gatA

Organism
Heliobacterium modesticaldum (strain ATCC 51547 / Ice1)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).UniRule annotation

Catalytic activityi

ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei79Charge relay systemUniRule annotation1
Active sitei154Charge relay systemUniRule annotation1
Active sitei178Acyl-ester intermediateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamyl-tRNA(Gln) amidotransferase subunit AUniRule annotation (EC:6.3.5.7UniRule annotation)
Short name:
Glu-ADT subunit AUniRule annotation
Gene namesi
Name:gatAUniRule annotation
Ordered Locus Names:Helmi_29070
ORF Names:HM1_3025
OrganismiHeliobacterium modesticaldum (strain ATCC 51547 / Ice1)
Taxonomic identifieri498761 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesHeliobacteriaceaeHeliobacterium
Proteomesi
  • UP000008550 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000951371 – 487Glutamyl-tRNA(Gln) amidotransferase subunit AAdd BLAST487

Interactioni

Subunit structurei

Heterotrimer of A, B and C subunits.UniRule annotation

Protein-protein interaction databases

STRINGi498761.HM1_3025.

Structurei

3D structure databases

ProteinModelPortaliB0TDK7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the amidase family. GatA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C3P. Bacteria.
COG0154. LUCA.
HOGENOMiHOG000116699.
KOiK02433.
OMAiKEYFGAG.
OrthoDBiPOG091H00O9.

Family and domain databases

Gene3Di3.90.1300.10. 1 hit.
HAMAPiMF_00120. GatA. 1 hit.
InterProiIPR000120. Amidase.
IPR020556. Amidase_CS.
IPR023631. Amidase_dom.
IPR004412. GatA.
[Graphical view]
PANTHERiPTHR11895. PTHR11895. 1 hit.
PfamiPF01425. Amidase. 1 hit.
[Graphical view]
SUPFAMiSSF75304. SSF75304. 1 hit.
TIGRFAMsiTIGR00132. gatA. 1 hit.
PROSITEiPS00571. AMIDASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B0TDK7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLYTKTAHE LHDLLVRKEV SATEIVKTQA DRMQALEPKI RAFVTLTVDK
60 70 80 90 100
ALEQAARVDA KIAAGEAIGP LEGIPMAIKD NMCTDGVRTT CSSKILNNFV
110 120 130 140 150
PPYDATVVTK LKEAGAVMMG KTNLDEFAMG SSTENSGFFA TCNPWDIERV
160 170 180 190 200
PGGSSGGSAA SVAAGQAVFS LGSDTGGSIR QPAAFCGVVG LKPTYGAVSR
210 220 230 240 250
FGLIAFASSL DQIGPFTRDV RDCAHVMNAI AGHDAKDSTS APVDYPDYTS
260 270 280 290 300
LLGKPIKGWR VGLPKEYFGD GMDPEVKEVI EKAVKTIEDL GAEVAECSLP
310 320 330 340 350
HTEYALPVYY LIAPAEASSN LARYDGVRYG YRSESADDLI TMFKKTRAEG
360 370 380 390 400
FGDEVKRRIM LGTYALSSGY YDAYYLKALK VRTLIKNDFD QAFEKFDVIL
410 420 430 440 450
SPTTPTVAFK FGDRTDNPLQ MYLSDIYTLS VNLAGIPGLS INAGFAKGMP
460 470 480
VGLQIIGKPF DEARLLQIAY AYEEATGCHS KMPDLGV
Length:487
Mass (Da):52,524
Last modified:April 8, 2008 - v1
Checksum:i63A88959D0F5E8E1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000930 Genomic DNA. Translation: ABZ85532.1.
RefSeqiWP_012284007.1. NC_010337.2.

Genome annotation databases

EnsemblBacteriaiABZ85532; ABZ85532; HM1_3025.
KEGGihmo:HM1_3025.
PATRICi22110017. VBIHelMod36755_2692.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000930 Genomic DNA. Translation: ABZ85532.1.
RefSeqiWP_012284007.1. NC_010337.2.

3D structure databases

ProteinModelPortaliB0TDK7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi498761.HM1_3025.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABZ85532; ABZ85532; HM1_3025.
KEGGihmo:HM1_3025.
PATRICi22110017. VBIHelMod36755_2692.

Phylogenomic databases

eggNOGiENOG4105C3P. Bacteria.
COG0154. LUCA.
HOGENOMiHOG000116699.
KOiK02433.
OMAiKEYFGAG.
OrthoDBiPOG091H00O9.

Family and domain databases

Gene3Di3.90.1300.10. 1 hit.
HAMAPiMF_00120. GatA. 1 hit.
InterProiIPR000120. Amidase.
IPR020556. Amidase_CS.
IPR023631. Amidase_dom.
IPR004412. GatA.
[Graphical view]
PANTHERiPTHR11895. PTHR11895. 1 hit.
PfamiPF01425. Amidase. 1 hit.
[Graphical view]
SUPFAMiSSF75304. SSF75304. 1 hit.
TIGRFAMsiTIGR00132. gatA. 1 hit.
PROSITEiPS00571. AMIDASES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGATA_HELMI
AccessioniPrimary (citable) accession number: B0TDK7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: April 8, 2008
Last modified: November 2, 2016
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.