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B0TDI1

- ADDB_HELMI

UniProt

B0TDI1 - ADDB_HELMI

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Protein

ATP-dependent helicase/deoxyribonuclease subunit B

Gene

addB

Organism
Heliobacterium modesticaldum (strain ATCC 51547 / Ice1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

The heterodimer acts as both an ATP-dependent DNA helicase and an ATP-dependent, dual-direction single-stranded exonuclease. Recognizes the chi site generating a DNA molecule suitable for the initiation of homologous recombination. The AddB nuclease domain is not required for chi fragment generation; this subunit has 5' -> 3' nuclease activity.UniRule annotation

Catalytic activityi

ATP + H2O = ADP + phosphate.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster.UniRule annotation
Magnesium.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi841 – 8411Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi1176 – 11761Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi1179 – 11791Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi1185 – 11851Iron-sulfur (4Fe-4S)UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi8 – 158ATPUniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. 5'-3' exonuclease activity Source: UniProtKB-HAMAP
  3. ATP binding Source: UniProtKB-HAMAP
  4. double-stranded DNA binding Source: UniProtKB-HAMAP
  5. helicase activity Source: UniProtKB-HAMAP
  6. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. double-strand break repair via homologous recombination Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Exonuclease, Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

4Fe-4S, ATP-binding, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciHMOD498761:GI46-3012-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent helicase/deoxyribonuclease subunit BUniRule annotation (EC:3.1.-.-UniRule annotation, EC:3.6.4.12UniRule annotation)
Alternative name(s):
ATP-dependent helicase/nuclease AddBUniRule annotation
Gene namesi
Name:addBUniRule annotation
Ordered Locus Names:Helmi_28810
ORF Names:HM1_2997
OrganismiHeliobacterium modesticaldum (strain ATCC 51547 / Ice1)
Taxonomic identifieri498761 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesHeliobacteriaceaeHeliobacterium
ProteomesiUP000008550: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12241224ATP-dependent helicase/deoxyribonuclease subunit BPRO_0000379193Add
BLAST

Interactioni

Subunit structurei

Heterodimer of AddA and AddB.UniRule annotation

Protein-protein interaction databases

STRINGi498761.HM1_2997.

Structurei

3D structure databases

ProteinModelPortaliB0TDI1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 326326UvrD-like helicase ATP-bindingUniRule annotationAdd
BLAST
Domaini283 – 584302UvrD-like helicase C-terminalUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the helicase family. AddB/RexB type 1 subfamily.UniRule annotation
Contains 1 uvrD-like helicase ATP-binding domain.UniRule annotation
Contains 1 uvrD-like helicase C-terminal domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG3857.
HOGENOMiHOG000285805.
KOiK16899.
OMAiVPDQMTF.
OrthoDBiEOG6DC6FN.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_01452. AddB_type1.
InterProiIPR014140. DNA_helicase_suAddB.
IPR014017. DNA_helicase_UvrD-like_C.
IPR027417. P-loop_NTPase.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS51198. UVRD_HELICASE_ATP_BIND. 1 hit.
PS51217. UVRD_HELICASE_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B0TDI1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSLRFILGRA GTGKSRACLD EIAQELKGSP QGPPLLFLVP EQATFQTERA
60 70 80 90 100
LATMPGLGGT IRAQVFSFRR LAYRVLQEVG GSARIPIGDL GKRMILRNLL
110 120 130 140 150
EKHKDELRLF HRSAGQPGFA DALAGALSEL KLYNIDATSL DRCQTEVIER
160 170 180 190 200
QGAGLLADKL HDLALLYGAL KTFLEGKYTD PDDYLHLLAQ RIPGSVMLRD
210 220 230 240 250
AEVWVDGFAG FTPQELSVLA ALMSACRRVN VALCLDPTEL DEPCDLDDPF
260 270 280 290 300
FRTRETQAKL LQLAFDRGIV IEKALHLVPA PGQSAPRFQH PELQHLESFY
310 320 330 340 350
FRRPAPPWLG GTQSLFVCAA ANRRSEVEGA AREIIRLCRD QGYRWRDVAV
360 370 380 390 400
LLRDLESYYD SIESVFSDFG IPLFIDSKRP APHHPLVELI RSALEAVLRD
410 420 430 440 450
WAYEPVFRYL KTDLVPLSRG ETDRLENYVL AHGIRGRRWR DREPWLFRRR
460 470 480 490 500
LTLGEDALSE FSDQELAELA EINGARDRAR AALASFHKAV VGAKAVRPIT
510 520 530 540 550
AALYQLLVDL KAPEQISCWI AEAEAAGRIE EAHAHRQVWA AVIDLFDQIV
560 570 580 590 600
ESLGETPLDL ATYAVVMEAG LDSLKLSLIP PELDQVFVGS LDRSRCPGIR
610 620 630 640 650
AAFVLGVSEG VLPARPKDDG IFDDKERERI YALTGLELAP GSRRRLFDEE
660 670 680 690 700
YHIYVALTRA SERCYLSYPL ADAEGRAQLP SSVIARVREL FPQVTERTWL
710 720 730 740 750
LEPGSLPTGA GLSGSAFAGA ATAETAAMAQ TALAARTTKE AAMSAVEREL
760 770 780 790 800
AEEYIVHPAR SLSYLIPSLR EALARRPVPL VWWAVYSWLA ERPAWRELLA
810 820 830 840 850
RVLSGLWHKN REQDLPKPVS RRLFGDPLRA SVSRIERFLA CPFSHFISHG
860 870 880 890 900
LRLKERRIFR LAAPDLGEFF HAALKQFGER LRRDRLDWGA LPREELFRLT
910 920 930 940 950
GEVVSDLAPQ LQNEILLSTA RHRYLTGRLQ QTLSRAVIVL GEHARRGQFR
960 970 980 990 1000
PLALEVGFGP EELLPPVQVP LTGGRWMDLV GRIDRIDGFF VGDASWLRVI
1010 1020 1030 1040 1050
DYKSNRANLK LADIAHGLKL QLLAYLDVAL RHGNRLLQGC ALGEDRPSSL
1060 1070 1080 1090 1100
SAATACHPGG MLYFGICDPL LSTGGPLTRD EAERAILKEA KMCGHVLADP
1110 1120 1130 1140 1150
AVVAAMDGQT RQGYSPLIPV GLKKEGGFYS ASSVLTMEQF ALLRQYLQSI
1160 1170 1180 1190 1200
VARVGEAILD GLVSIRPHRR DDRLACSFCP YKSVCQFDLL LQDNDYRLLI
1210 1220
DDPPEKIWEK IAAGPKAIFE GVIR
Length:1,224
Mass (Da):136,519
Last modified:April 8, 2008 - v1
Checksum:iD541C4A06B9E0F0E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000930 Genomic DNA. Translation: ABZ85506.1.
RefSeqiYP_001681517.1. NC_010337.2.

Genome annotation databases

EnsemblBacteriaiABZ85506; ABZ85506; HM1_2997.
GeneIDi5911241.
KEGGihmo:HM1_2997.
PATRICi22109967. VBIHelMod36755_2667.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000930 Genomic DNA. Translation: ABZ85506.1 .
RefSeqi YP_001681517.1. NC_010337.2.

3D structure databases

ProteinModelPortali B0TDI1.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 498761.HM1_2997.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABZ85506 ; ABZ85506 ; HM1_2997 .
GeneIDi 5911241.
KEGGi hmo:HM1_2997.
PATRICi 22109967. VBIHelMod36755_2667.

Phylogenomic databases

eggNOGi COG3857.
HOGENOMi HOG000285805.
KOi K16899.
OMAi VPDQMTF.
OrthoDBi EOG6DC6FN.

Enzyme and pathway databases

BioCyci HMOD498761:GI46-3012-MONOMER.

Family and domain databases

Gene3Di 3.40.50.300. 2 hits.
HAMAPi MF_01452. AddB_type1.
InterProi IPR014140. DNA_helicase_suAddB.
IPR014017. DNA_helicase_UvrD-like_C.
IPR027417. P-loop_NTPase.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
PROSITEi PS51198. UVRD_HELICASE_ATP_BIND. 1 hit.
PS51217. UVRD_HELICASE_CTER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome of Heliobacterium modesticaldum, a phototrophic representative of the Firmicutes containing the simplest photosynthetic apparatus."
    Sattley W.M., Madigan M.T., Swingley W.D., Cheung P.C., Clocksin K.M., Conrad A.L., Dejesa L.C., Honchak B.M., Jung D.O., Karbach L.E., Kurdoglu A., Lahiri S., Mastrian S.D., Page L.E., Taylor H.L., Wang Z.T., Raymond J., Chen M., Blankenship R.E., Touchman J.W.
    J. Bacteriol. 190:4687-4696(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 51547 / Ice1.

Entry informationi

Entry nameiADDB_HELMI
AccessioniPrimary (citable) accession number: B0TDI1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 7, 2009
Last sequence update: April 8, 2008
Last modified: October 1, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3