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Protein

ATP-dependent helicase/deoxyribonuclease subunit B

Gene

addB

Organism
Heliobacterium modesticaldum (strain ATCC 51547 / Ice1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

The heterodimer acts as both an ATP-dependent DNA helicase and an ATP-dependent, dual-direction single-stranded exonuclease. Recognizes the chi site generating a DNA molecule suitable for the initiation of homologous recombination. The AddB nuclease domain is not required for chi fragment generation; this subunit has 5' -> 3' nuclease activity.UniRule annotation

Catalytic activityi

ATP + H2O = ADP + phosphate.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation
  • Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi841Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi1176Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi1179Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi1185Iron-sulfur (4Fe-4S)UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi8 – 15ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionExonuclease, Hydrolase, Nuclease
Biological processDNA damage, DNA repair
Ligand4Fe-4S, ATP-binding, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciHMOD498761:G1GB0-2759-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent helicase/deoxyribonuclease subunit BUniRule annotation (EC:3.1.-.-UniRule annotation, EC:3.6.4.12UniRule annotation)
Alternative name(s):
ATP-dependent helicase/nuclease AddBUniRule annotation
Gene namesi
Name:addBUniRule annotation
Ordered Locus Names:Helmi_28810
ORF Names:HM1_2997
OrganismiHeliobacterium modesticaldum (strain ATCC 51547 / Ice1)
Taxonomic identifieri498761 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesHeliobacteriaceaeHeliobacterium
Proteomesi
  • UP000008550 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003791931 – 1224ATP-dependent helicase/deoxyribonuclease subunit BAdd BLAST1224

Interactioni

Subunit structurei

Heterodimer of AddA and AddB.UniRule annotation

Protein-protein interaction databases

STRINGi498761.HM1_2997

Structurei

3D structure databases

ProteinModelPortaliB0TDI1
SMRiB0TDI1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 326UvrD-like helicase ATP-bindingUniRule annotationAdd BLAST326
Domaini283 – 584UvrD-like helicase C-terminalUniRule annotationAdd BLAST302

Sequence similaritiesi

Belongs to the helicase family. AddB/RexB type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C5N Bacteria
COG3857 LUCA
HOGENOMiHOG000285805
KOiK16899
OMAiHFASHGL
OrthoDBiPOG091H01PC

Family and domain databases

HAMAPiMF_01452 AddB_type1, 1 hit
InterProiView protein in InterPro
IPR014140 DNA_helicase_suAddB
IPR014017 DNA_helicase_UvrD-like_C
IPR027417 P-loop_NTPase
IPR038726 PDDEXK_AddAB-type
IPR014016 UvrD-like_ATP-bd
PfamiView protein in Pfam
PF12705 PDDEXK_1, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS51198 UVRD_HELICASE_ATP_BIND, 1 hit
PS51217 UVRD_HELICASE_CTER, 1 hit

Sequencei

Sequence statusi: Complete.

B0TDI1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLRFILGRA GTGKSRACLD EIAQELKGSP QGPPLLFLVP EQATFQTERA
60 70 80 90 100
LATMPGLGGT IRAQVFSFRR LAYRVLQEVG GSARIPIGDL GKRMILRNLL
110 120 130 140 150
EKHKDELRLF HRSAGQPGFA DALAGALSEL KLYNIDATSL DRCQTEVIER
160 170 180 190 200
QGAGLLADKL HDLALLYGAL KTFLEGKYTD PDDYLHLLAQ RIPGSVMLRD
210 220 230 240 250
AEVWVDGFAG FTPQELSVLA ALMSACRRVN VALCLDPTEL DEPCDLDDPF
260 270 280 290 300
FRTRETQAKL LQLAFDRGIV IEKALHLVPA PGQSAPRFQH PELQHLESFY
310 320 330 340 350
FRRPAPPWLG GTQSLFVCAA ANRRSEVEGA AREIIRLCRD QGYRWRDVAV
360 370 380 390 400
LLRDLESYYD SIESVFSDFG IPLFIDSKRP APHHPLVELI RSALEAVLRD
410 420 430 440 450
WAYEPVFRYL KTDLVPLSRG ETDRLENYVL AHGIRGRRWR DREPWLFRRR
460 470 480 490 500
LTLGEDALSE FSDQELAELA EINGARDRAR AALASFHKAV VGAKAVRPIT
510 520 530 540 550
AALYQLLVDL KAPEQISCWI AEAEAAGRIE EAHAHRQVWA AVIDLFDQIV
560 570 580 590 600
ESLGETPLDL ATYAVVMEAG LDSLKLSLIP PELDQVFVGS LDRSRCPGIR
610 620 630 640 650
AAFVLGVSEG VLPARPKDDG IFDDKERERI YALTGLELAP GSRRRLFDEE
660 670 680 690 700
YHIYVALTRA SERCYLSYPL ADAEGRAQLP SSVIARVREL FPQVTERTWL
710 720 730 740 750
LEPGSLPTGA GLSGSAFAGA ATAETAAMAQ TALAARTTKE AAMSAVEREL
760 770 780 790 800
AEEYIVHPAR SLSYLIPSLR EALARRPVPL VWWAVYSWLA ERPAWRELLA
810 820 830 840 850
RVLSGLWHKN REQDLPKPVS RRLFGDPLRA SVSRIERFLA CPFSHFISHG
860 870 880 890 900
LRLKERRIFR LAAPDLGEFF HAALKQFGER LRRDRLDWGA LPREELFRLT
910 920 930 940 950
GEVVSDLAPQ LQNEILLSTA RHRYLTGRLQ QTLSRAVIVL GEHARRGQFR
960 970 980 990 1000
PLALEVGFGP EELLPPVQVP LTGGRWMDLV GRIDRIDGFF VGDASWLRVI
1010 1020 1030 1040 1050
DYKSNRANLK LADIAHGLKL QLLAYLDVAL RHGNRLLQGC ALGEDRPSSL
1060 1070 1080 1090 1100
SAATACHPGG MLYFGICDPL LSTGGPLTRD EAERAILKEA KMCGHVLADP
1110 1120 1130 1140 1150
AVVAAMDGQT RQGYSPLIPV GLKKEGGFYS ASSVLTMEQF ALLRQYLQSI
1160 1170 1180 1190 1200
VARVGEAILD GLVSIRPHRR DDRLACSFCP YKSVCQFDLL LQDNDYRLLI
1210 1220
DDPPEKIWEK IAAGPKAIFE GVIR
Length:1,224
Mass (Da):136,519
Last modified:April 8, 2008 - v1
Checksum:iD541C4A06B9E0F0E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000930 Genomic DNA Translation: ABZ85506.1
RefSeqiWP_012283981.1, NC_010337.2

Genome annotation databases

EnsemblBacteriaiABZ85506; ABZ85506; HM1_2997
KEGGihmo:HM1_2997

Entry informationi

Entry nameiADDB_HELMI
AccessioniPrimary (citable) accession number: B0TDI1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 7, 2009
Last sequence update: April 8, 2008
Last modified: April 25, 2018
This is version 67 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health