B0TDI1 (ADDB_HELMI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-dependent helicase/deoxyribonuclease subunit B
EC=3.1.-.-
EC=3.6.4.12

Alternative name(s):
ATP-dependent helicase/nuclease AddB

Gene names

Name:	addB
Ordered Locus Names:	Helmi_28810
ORF Names:	HM1_2997

Organism

Heliobacterium modesticaldum (strain ATCC 51547 / Ice1) [Complete proteome] [HAMAP]

Taxonomic identifier

498761 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Clostridia › Clostridiales › Heliobacteriaceae › Heliobacterium › Heliobacterium modesticaldum

Protein attributes

Sequence length	1224 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The heterodimer acts as both an ATP-dependent DNA helicase and an ATP-dependent, dual-direction single-stranded exonuclease. Recognizes the chi site generating a DNA molecule suitable for the initiation of homologous recombination. The AddB nuclease domain is not required for chi fragment generation; this subunit has 5' -> 3' nuclease activity By similarity. HAMAP-Rule MF_01452
Catalytic activity	ATP + H2O = ADP + phosphate. HAMAP-Rule MF_01452
Cofactor	Binds 1 4Fe-4S cluster. Magnesium By similarity. HAMAP-Rule MF_01452
Subunit structure	Heterodimer of AddA and AddB By similarity. HAMAP-Rule MF_01452
Sequence similarities	Belongs to the helicase family. AddB/RexB type 1 subfamily. Contains 1 uvrD-like helicase ATP-binding domain. Contains 1 uvrD-like helicase C-terminal domain.

Ontologies

Keywords
Biological process	DNA damage DNA repair
Ligand	4Fe-4S ATP-binding Iron Iron-sulfur Metal-binding Nucleotide-binding
Molecular function	Exonuclease Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	double-strand break repair via homologous recombination Inferred from electronic annotation. Source: HAMAP nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 5'-3' exonuclease activity Inferred from electronic annotation. Source: HAMAP ATP binding Inferred from electronic annotation. Source: HAMAP double-stranded DNA binding Inferred from electronic annotation. Source: HAMAP helicase activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1224	1224	ATP-dependent helicase/deoxyribonuclease subunit B HAMAP-Rule MF_01452		PRO_0000379193
Regions
	Domain	1 – 326	326	UvrD-like helicase ATP-binding
	Domain	283 – 584	302	UvrD-like helicase C-terminal
	Nucleotide binding	8 – 15	8	ATP By similarity
Sites
	Metal binding	841	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	1176	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	1179	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	1185	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	B0TDI1 [UniParc]. Last modified April 8, 2008. Version 1. Checksum: D541C4A06B9E0F0E	FASTA	1,224	136,519
	10 20 30 40 50 60 MSLRFILGRA GTGKSRACLD EIAQELKGSP QGPPLLFLVP EQATFQTERA LATMPGLGGT 70 80 90 100 110 120 IRAQVFSFRR LAYRVLQEVG GSARIPIGDL GKRMILRNLL EKHKDELRLF HRSAGQPGFA 130 140 150 160 170 180 DALAGALSEL KLYNIDATSL DRCQTEVIER QGAGLLADKL HDLALLYGAL KTFLEGKYTD 190 200 210 220 230 240 PDDYLHLLAQ RIPGSVMLRD AEVWVDGFAG FTPQELSVLA ALMSACRRVN VALCLDPTEL 250 260 270 280 290 300 DEPCDLDDPF FRTRETQAKL LQLAFDRGIV IEKALHLVPA PGQSAPRFQH PELQHLESFY 310 320 330 340 350 360 FRRPAPPWLG GTQSLFVCAA ANRRSEVEGA AREIIRLCRD QGYRWRDVAV LLRDLESYYD 370 380 390 400 410 420 SIESVFSDFG IPLFIDSKRP APHHPLVELI RSALEAVLRD WAYEPVFRYL KTDLVPLSRG 430 440 450 460 470 480 ETDRLENYVL AHGIRGRRWR DREPWLFRRR LTLGEDALSE FSDQELAELA EINGARDRAR 490 500 510 520 530 540 AALASFHKAV VGAKAVRPIT AALYQLLVDL KAPEQISCWI AEAEAAGRIE EAHAHRQVWA 550 560 570 580 590 600 AVIDLFDQIV ESLGETPLDL ATYAVVMEAG LDSLKLSLIP PELDQVFVGS LDRSRCPGIR 610 620 630 640 650 660 AAFVLGVSEG VLPARPKDDG IFDDKERERI YALTGLELAP GSRRRLFDEE YHIYVALTRA 670 680 690 700 710 720 SERCYLSYPL ADAEGRAQLP SSVIARVREL FPQVTERTWL LEPGSLPTGA GLSGSAFAGA 730 740 750 760 770 780 ATAETAAMAQ TALAARTTKE AAMSAVEREL AEEYIVHPAR SLSYLIPSLR EALARRPVPL 790 800 810 820 830 840 VWWAVYSWLA ERPAWRELLA RVLSGLWHKN REQDLPKPVS RRLFGDPLRA SVSRIERFLA 850 860 870 880 890 900 CPFSHFISHG LRLKERRIFR LAAPDLGEFF HAALKQFGER LRRDRLDWGA LPREELFRLT 910 920 930 940 950 960 GEVVSDLAPQ LQNEILLSTA RHRYLTGRLQ QTLSRAVIVL GEHARRGQFR PLALEVGFGP 970 980 990 1000 1010 1020 EELLPPVQVP LTGGRWMDLV GRIDRIDGFF VGDASWLRVI DYKSNRANLK LADIAHGLKL 1030 1040 1050 1060 1070 1080 QLLAYLDVAL RHGNRLLQGC ALGEDRPSSL SAATACHPGG MLYFGICDPL LSTGGPLTRD 1090 1100 1110 1120 1130 1140 EAERAILKEA KMCGHVLADP AVVAAMDGQT RQGYSPLIPV GLKKEGGFYS ASSVLTMEQF 1150 1160 1170 1180 1190 1200 ALLRQYLQSI VARVGEAILD GLVSIRPHRR DDRLACSFCP YKSVCQFDLL LQDNDYRLLI 1210 1220 DDPPEKIWEK IAAGPKAIFE GVIR

References

[1]

"The genome of Heliobacterium modesticaldum, a phototrophic representative of the Firmicutes containing the simplest photosynthetic apparatus." Sattley W.M., Madigan M.T., Swingley W.D., Cheung P.C., Clocksin K.M., Conrad A.L., Dejesa L.C., Honchak B.M., Jung D.O., Karbach L.E., Kurdoglu A., Lahiri S., Mastrian S.D., Page L.E., Taylor H.L., Wang Z.T., Raymond J., Chen M., Blankenship R.E., Touchman J.W. J. Bacteriol. 190:4687-4696(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51547 / Ice1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000930 Genomic DNA. Translation: ABZ85506.1.
RefSeq	YP_001681517.1. NC_010337.2.
3D structure databases
ProteinModelPortal	B0TDI1.
ModBase	Search...
Protein-protein interaction databases
STRING	498761.HM1_2997.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABZ85506; ABZ85506; HM1_2997.
GeneID	5911241.
KEGG	hmo:HM1_2997.
PATRIC	22109967. VBIHelMod36755_2667.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG3857.
HOGENOM	HOG000285805.
KO	K16899.
OMA	DIGNYKA.
ProtClustDB	CLSK968214.
Enzyme and pathway databases
BioCyc	HMOD498761:GI46-3012-MONOMER.
Family and domain databases
HAMAP	MF_01452. AddB_type1.
InterPro	IPR014140. DNA_helicase_suAddB. IPR014017. DNA_helicase_UvrD-like_C. IPR027417. P-loop_NTPase. [Graphical view]
SUPFAM	SSF52540. SSF52540. 1 hit.
PROSITE	PS51198. UVRD_HELICASE_ATP_BIND. 1 hit. PS51217. UVRD_HELICASE_CTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ADDB_HELMI

Accession

Primary (citable) accession number: B0TDI1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 7, 2009
Last sequence update:	April 8, 2008
Last modified:	May 29, 2013
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program