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B0TDI1

- ADDB_HELMI

UniProt

B0TDI1 - ADDB_HELMI

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Protein
ATP-dependent helicase/deoxyribonuclease subunit B
Gene
addB, Helmi_28810, HM1_2997
Organism
Heliobacterium modesticaldum (strain ATCC 51547 / Ice1)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

The heterodimer acts as both an ATP-dependent DNA helicase and an ATP-dependent, dual-direction single-stranded exonuclease. Recognizes the chi site generating a DNA molecule suitable for the initiation of homologous recombination. The AddB nuclease domain is not required for chi fragment generation; this subunit has 5' -> 3' nuclease activity By similarity.UniRule annotation

Catalytic activityi

ATP + H2O = ADP + phosphate.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster By similarity.UniRule annotation
Magnesium By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi841 – 8411Iron-sulfur (4Fe-4S) By similarity
Metal bindingi1176 – 11761Iron-sulfur (4Fe-4S) By similarity
Metal bindingi1179 – 11791Iron-sulfur (4Fe-4S) By similarity
Metal bindingi1185 – 11851Iron-sulfur (4Fe-4S) By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi8 – 158ATP By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. 5'-3' exonuclease activity Source: UniProtKB-HAMAP
  3. ATP binding Source: UniProtKB-HAMAP
  4. double-stranded DNA binding Source: UniProtKB-HAMAP
  5. helicase activity Source: UniProtKB-HAMAP
  6. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. double-strand break repair via homologous recombination Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Exonuclease, Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

4Fe-4S, ATP-binding, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciHMOD498761:GI46-3012-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent helicase/deoxyribonuclease subunit B (EC:3.1.-.-, EC:3.6.4.12)
Alternative name(s):
ATP-dependent helicase/nuclease AddB
Gene namesi
Name:addB
Ordered Locus Names:Helmi_28810
ORF Names:HM1_2997
OrganismiHeliobacterium modesticaldum (strain ATCC 51547 / Ice1)
Taxonomic identifieri498761 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesHeliobacteriaceaeHeliobacterium
ProteomesiUP000008550: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12241224ATP-dependent helicase/deoxyribonuclease subunit BUniRule annotation
PRO_0000379193Add
BLAST

Interactioni

Subunit structurei

Heterodimer of AddA and AddB By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi498761.HM1_2997.

Structurei

3D structure databases

ProteinModelPortaliB0TDI1.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 326326UvrD-like helicase ATP-binding
Add
BLAST
Domaini283 – 584302UvrD-like helicase C-terminal
Add
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG3857.
HOGENOMiHOG000285805.
KOiK16899.
OMAiVPDQMTF.
OrthoDBiEOG6DC6FN.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_01452. AddB_type1.
InterProiIPR014140. DNA_helicase_suAddB.
IPR014017. DNA_helicase_UvrD-like_C.
IPR027417. P-loop_NTPase.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS51198. UVRD_HELICASE_ATP_BIND. 1 hit.
PS51217. UVRD_HELICASE_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B0TDI1-1 [UniParc]FASTAAdd to Basket

« Hide

MSLRFILGRA GTGKSRACLD EIAQELKGSP QGPPLLFLVP EQATFQTERA     50
LATMPGLGGT IRAQVFSFRR LAYRVLQEVG GSARIPIGDL GKRMILRNLL 100
EKHKDELRLF HRSAGQPGFA DALAGALSEL KLYNIDATSL DRCQTEVIER 150
QGAGLLADKL HDLALLYGAL KTFLEGKYTD PDDYLHLLAQ RIPGSVMLRD 200
AEVWVDGFAG FTPQELSVLA ALMSACRRVN VALCLDPTEL DEPCDLDDPF 250
FRTRETQAKL LQLAFDRGIV IEKALHLVPA PGQSAPRFQH PELQHLESFY 300
FRRPAPPWLG GTQSLFVCAA ANRRSEVEGA AREIIRLCRD QGYRWRDVAV 350
LLRDLESYYD SIESVFSDFG IPLFIDSKRP APHHPLVELI RSALEAVLRD 400
WAYEPVFRYL KTDLVPLSRG ETDRLENYVL AHGIRGRRWR DREPWLFRRR 450
LTLGEDALSE FSDQELAELA EINGARDRAR AALASFHKAV VGAKAVRPIT 500
AALYQLLVDL KAPEQISCWI AEAEAAGRIE EAHAHRQVWA AVIDLFDQIV 550
ESLGETPLDL ATYAVVMEAG LDSLKLSLIP PELDQVFVGS LDRSRCPGIR 600
AAFVLGVSEG VLPARPKDDG IFDDKERERI YALTGLELAP GSRRRLFDEE 650
YHIYVALTRA SERCYLSYPL ADAEGRAQLP SSVIARVREL FPQVTERTWL 700
LEPGSLPTGA GLSGSAFAGA ATAETAAMAQ TALAARTTKE AAMSAVEREL 750
AEEYIVHPAR SLSYLIPSLR EALARRPVPL VWWAVYSWLA ERPAWRELLA 800
RVLSGLWHKN REQDLPKPVS RRLFGDPLRA SVSRIERFLA CPFSHFISHG 850
LRLKERRIFR LAAPDLGEFF HAALKQFGER LRRDRLDWGA LPREELFRLT 900
GEVVSDLAPQ LQNEILLSTA RHRYLTGRLQ QTLSRAVIVL GEHARRGQFR 950
PLALEVGFGP EELLPPVQVP LTGGRWMDLV GRIDRIDGFF VGDASWLRVI 1000
DYKSNRANLK LADIAHGLKL QLLAYLDVAL RHGNRLLQGC ALGEDRPSSL 1050
SAATACHPGG MLYFGICDPL LSTGGPLTRD EAERAILKEA KMCGHVLADP 1100
AVVAAMDGQT RQGYSPLIPV GLKKEGGFYS ASSVLTMEQF ALLRQYLQSI 1150
VARVGEAILD GLVSIRPHRR DDRLACSFCP YKSVCQFDLL LQDNDYRLLI 1200
DDPPEKIWEK IAAGPKAIFE GVIR 1224
Length:1,224
Mass (Da):136,519
Last modified:April 8, 2008 - v1
Checksum:iD541C4A06B9E0F0E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000930 Genomic DNA. Translation: ABZ85506.1.
RefSeqiYP_001681517.1. NC_010337.2.

Genome annotation databases

EnsemblBacteriaiABZ85506; ABZ85506; HM1_2997.
GeneIDi5911241.
KEGGihmo:HM1_2997.
PATRICi22109967. VBIHelMod36755_2667.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000930 Genomic DNA. Translation: ABZ85506.1 .
RefSeqi YP_001681517.1. NC_010337.2.

3D structure databases

ProteinModelPortali B0TDI1.
ModBasei Search...

Protein-protein interaction databases

STRINGi 498761.HM1_2997.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABZ85506 ; ABZ85506 ; HM1_2997 .
GeneIDi 5911241.
KEGGi hmo:HM1_2997.
PATRICi 22109967. VBIHelMod36755_2667.

Phylogenomic databases

eggNOGi COG3857.
HOGENOMi HOG000285805.
KOi K16899.
OMAi VPDQMTF.
OrthoDBi EOG6DC6FN.

Enzyme and pathway databases

BioCyci HMOD498761:GI46-3012-MONOMER.

Family and domain databases

Gene3Di 3.40.50.300. 2 hits.
HAMAPi MF_01452. AddB_type1.
InterProi IPR014140. DNA_helicase_suAddB.
IPR014017. DNA_helicase_UvrD-like_C.
IPR027417. P-loop_NTPase.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
PROSITEi PS51198. UVRD_HELICASE_ATP_BIND. 1 hit.
PS51217. UVRD_HELICASE_CTER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome of Heliobacterium modesticaldum, a phototrophic representative of the Firmicutes containing the simplest photosynthetic apparatus."
    Sattley W.M., Madigan M.T., Swingley W.D., Cheung P.C., Clocksin K.M., Conrad A.L., Dejesa L.C., Honchak B.M., Jung D.O., Karbach L.E., Kurdoglu A., Lahiri S., Mastrian S.D., Page L.E., Taylor H.L., Wang Z.T., Raymond J., Chen M., Blankenship R.E., Touchman J.W.
    J. Bacteriol. 190:4687-4696(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 51547 / Ice1.

Entry informationi

Entry nameiADDB_HELMI
AccessioniPrimary (citable) accession number: B0TDI1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 7, 2009
Last sequence update: April 8, 2008
Last modified: May 14, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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