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B0TDI1

- ADDB_HELMI

UniProt

B0TDI1 - ADDB_HELMI

Protein

ATP-dependent helicase/deoxyribonuclease subunit B

Gene

addB

Organism
Heliobacterium modesticaldum (strain ATCC 51547 / Ice1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 46 (01 Oct 2014)
      Sequence version 1 (08 Apr 2008)
      Previous versions | rss
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    Functioni

    The heterodimer acts as both an ATP-dependent DNA helicase and an ATP-dependent, dual-direction single-stranded exonuclease. Recognizes the chi site generating a DNA molecule suitable for the initiation of homologous recombination. The AddB nuclease domain is not required for chi fragment generation; this subunit has 5' -> 3' nuclease activity.UniRule annotation

    Catalytic activityi

    ATP + H2O = ADP + phosphate.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster.UniRule annotation
    Magnesium.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi841 – 8411Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi1176 – 11761Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi1179 – 11791Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi1185 – 11851Iron-sulfur (4Fe-4S)UniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi8 – 158ATPUniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    2. 5'-3' exonuclease activity Source: UniProtKB-HAMAP
    3. ATP binding Source: UniProtKB-HAMAP
    4. double-stranded DNA binding Source: UniProtKB-HAMAP
    5. helicase activity Source: UniProtKB-HAMAP
    6. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. double-strand break repair via homologous recombination Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Exonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    DNA damage, DNA repair

    Keywords - Ligandi

    4Fe-4S, ATP-binding, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciHMOD498761:GI46-3012-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-dependent helicase/deoxyribonuclease subunit BUniRule annotation (EC:3.1.-.-UniRule annotation, EC:3.6.4.12UniRule annotation)
    Alternative name(s):
    ATP-dependent helicase/nuclease AddBUniRule annotation
    Gene namesi
    Name:addBUniRule annotation
    Ordered Locus Names:Helmi_28810
    ORF Names:HM1_2997
    OrganismiHeliobacterium modesticaldum (strain ATCC 51547 / Ice1)
    Taxonomic identifieri498761 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesHeliobacteriaceaeHeliobacterium
    ProteomesiUP000008550: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 12241224ATP-dependent helicase/deoxyribonuclease subunit BPRO_0000379193Add
    BLAST

    Interactioni

    Subunit structurei

    Heterodimer of AddA and AddB.UniRule annotation

    Protein-protein interaction databases

    STRINGi498761.HM1_2997.

    Structurei

    3D structure databases

    ProteinModelPortaliB0TDI1.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 326326UvrD-like helicase ATP-bindingUniRule annotationAdd
    BLAST
    Domaini283 – 584302UvrD-like helicase C-terminalUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the helicase family. AddB/RexB type 1 subfamily.UniRule annotation
    Contains 1 uvrD-like helicase ATP-binding domain.UniRule annotation
    Contains 1 uvrD-like helicase C-terminal domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG3857.
    HOGENOMiHOG000285805.
    KOiK16899.
    OMAiVPDQMTF.
    OrthoDBiEOG6DC6FN.

    Family and domain databases

    Gene3Di3.40.50.300. 2 hits.
    HAMAPiMF_01452. AddB_type1.
    InterProiIPR014140. DNA_helicase_suAddB.
    IPR014017. DNA_helicase_UvrD-like_C.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    PROSITEiPS51198. UVRD_HELICASE_ATP_BIND. 1 hit.
    PS51217. UVRD_HELICASE_CTER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B0TDI1-1 [UniParc]FASTAAdd to Basket

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    MSLRFILGRA GTGKSRACLD EIAQELKGSP QGPPLLFLVP EQATFQTERA     50
    LATMPGLGGT IRAQVFSFRR LAYRVLQEVG GSARIPIGDL GKRMILRNLL 100
    EKHKDELRLF HRSAGQPGFA DALAGALSEL KLYNIDATSL DRCQTEVIER 150
    QGAGLLADKL HDLALLYGAL KTFLEGKYTD PDDYLHLLAQ RIPGSVMLRD 200
    AEVWVDGFAG FTPQELSVLA ALMSACRRVN VALCLDPTEL DEPCDLDDPF 250
    FRTRETQAKL LQLAFDRGIV IEKALHLVPA PGQSAPRFQH PELQHLESFY 300
    FRRPAPPWLG GTQSLFVCAA ANRRSEVEGA AREIIRLCRD QGYRWRDVAV 350
    LLRDLESYYD SIESVFSDFG IPLFIDSKRP APHHPLVELI RSALEAVLRD 400
    WAYEPVFRYL KTDLVPLSRG ETDRLENYVL AHGIRGRRWR DREPWLFRRR 450
    LTLGEDALSE FSDQELAELA EINGARDRAR AALASFHKAV VGAKAVRPIT 500
    AALYQLLVDL KAPEQISCWI AEAEAAGRIE EAHAHRQVWA AVIDLFDQIV 550
    ESLGETPLDL ATYAVVMEAG LDSLKLSLIP PELDQVFVGS LDRSRCPGIR 600
    AAFVLGVSEG VLPARPKDDG IFDDKERERI YALTGLELAP GSRRRLFDEE 650
    YHIYVALTRA SERCYLSYPL ADAEGRAQLP SSVIARVREL FPQVTERTWL 700
    LEPGSLPTGA GLSGSAFAGA ATAETAAMAQ TALAARTTKE AAMSAVEREL 750
    AEEYIVHPAR SLSYLIPSLR EALARRPVPL VWWAVYSWLA ERPAWRELLA 800
    RVLSGLWHKN REQDLPKPVS RRLFGDPLRA SVSRIERFLA CPFSHFISHG 850
    LRLKERRIFR LAAPDLGEFF HAALKQFGER LRRDRLDWGA LPREELFRLT 900
    GEVVSDLAPQ LQNEILLSTA RHRYLTGRLQ QTLSRAVIVL GEHARRGQFR 950
    PLALEVGFGP EELLPPVQVP LTGGRWMDLV GRIDRIDGFF VGDASWLRVI 1000
    DYKSNRANLK LADIAHGLKL QLLAYLDVAL RHGNRLLQGC ALGEDRPSSL 1050
    SAATACHPGG MLYFGICDPL LSTGGPLTRD EAERAILKEA KMCGHVLADP 1100
    AVVAAMDGQT RQGYSPLIPV GLKKEGGFYS ASSVLTMEQF ALLRQYLQSI 1150
    VARVGEAILD GLVSIRPHRR DDRLACSFCP YKSVCQFDLL LQDNDYRLLI 1200
    DDPPEKIWEK IAAGPKAIFE GVIR 1224
    Length:1,224
    Mass (Da):136,519
    Last modified:April 8, 2008 - v1
    Checksum:iD541C4A06B9E0F0E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000930 Genomic DNA. Translation: ABZ85506.1.
    RefSeqiYP_001681517.1. NC_010337.2.

    Genome annotation databases

    EnsemblBacteriaiABZ85506; ABZ85506; HM1_2997.
    GeneIDi5911241.
    KEGGihmo:HM1_2997.
    PATRICi22109967. VBIHelMod36755_2667.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000930 Genomic DNA. Translation: ABZ85506.1 .
    RefSeqi YP_001681517.1. NC_010337.2.

    3D structure databases

    ProteinModelPortali B0TDI1.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 498761.HM1_2997.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABZ85506 ; ABZ85506 ; HM1_2997 .
    GeneIDi 5911241.
    KEGGi hmo:HM1_2997.
    PATRICi 22109967. VBIHelMod36755_2667.

    Phylogenomic databases

    eggNOGi COG3857.
    HOGENOMi HOG000285805.
    KOi K16899.
    OMAi VPDQMTF.
    OrthoDBi EOG6DC6FN.

    Enzyme and pathway databases

    BioCyci HMOD498761:GI46-3012-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.300. 2 hits.
    HAMAPi MF_01452. AddB_type1.
    InterProi IPR014140. DNA_helicase_suAddB.
    IPR014017. DNA_helicase_UvrD-like_C.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    PROSITEi PS51198. UVRD_HELICASE_ATP_BIND. 1 hit.
    PS51217. UVRD_HELICASE_CTER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome of Heliobacterium modesticaldum, a phototrophic representative of the Firmicutes containing the simplest photosynthetic apparatus."
      Sattley W.M., Madigan M.T., Swingley W.D., Cheung P.C., Clocksin K.M., Conrad A.L., Dejesa L.C., Honchak B.M., Jung D.O., Karbach L.E., Kurdoglu A., Lahiri S., Mastrian S.D., Page L.E., Taylor H.L., Wang Z.T., Raymond J., Chen M., Blankenship R.E., Touchman J.W.
      J. Bacteriol. 190:4687-4696(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 51547 / Ice1.

    Entry informationi

    Entry nameiADDB_HELMI
    AccessioniPrimary (citable) accession number: B0TDI1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 7, 2009
    Last sequence update: April 8, 2008
    Last modified: October 1, 2014
    This is version 46 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3