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B0TDI1 (ADDB_HELMI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
ATP-dependent helicase/deoxyribonuclease subunit B

EC=3.1.-.-
EC=3.6.4.12
Alternative name(s):
ATP-dependent helicase/nuclease AddB
Gene names
Name:addB
Ordered Locus Names:Helmi_28810
ORF Names:HM1_2997
OrganismHeliobacterium modesticaldum (strain ATCC 51547 / Ice1) [Complete proteome] [HAMAP]
Taxonomic identifier498761 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesHeliobacteriaceaeHeliobacterium

Protein attributes

Sequence length1224 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

The heterodimer acts as both an ATP-dependent DNA helicase and an ATP-dependent, dual-direction single-stranded exonuclease. Recognizes the chi site generating a DNA molecule suitable for the initiation of homologous recombination. The AddB nuclease domain is not required for chi fragment generation; this subunit has 5' -> 3' nuclease activity By similarity. HAMAP-Rule MF_01452

Catalytic activity

ATP + H2O = ADP + phosphate. HAMAP-Rule MF_01452

Cofactor

Binds 1 4Fe-4S cluster By similarity. HAMAP-Rule MF_01452

Magnesium By similarity. HAMAP-Rule MF_01452

Subunit structure

Heterodimer of AddA and AddB By similarity. HAMAP-Rule MF_01452

Sequence similarities

Belongs to the helicase family. AddB/RexB type 1 subfamily.

Contains 1 uvrD-like helicase ATP-binding domain.

Contains 1 uvrD-like helicase C-terminal domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12241224ATP-dependent helicase/deoxyribonuclease subunit B HAMAP-Rule MF_01452
PRO_0000379193

Regions

Domain1 – 326326UvrD-like helicase ATP-binding
Domain283 – 584302UvrD-like helicase C-terminal
Nucleotide binding8 – 158ATP By similarity

Sites

Metal binding8411Iron-sulfur (4Fe-4S) By similarity
Metal binding11761Iron-sulfur (4Fe-4S) By similarity
Metal binding11791Iron-sulfur (4Fe-4S) By similarity
Metal binding11851Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
B0TDI1 [UniParc].

Last modified April 8, 2008. Version 1.
Checksum: D541C4A06B9E0F0E

FASTA1,224136,519
        10         20         30         40         50         60 
MSLRFILGRA GTGKSRACLD EIAQELKGSP QGPPLLFLVP EQATFQTERA LATMPGLGGT 

        70         80         90        100        110        120 
IRAQVFSFRR LAYRVLQEVG GSARIPIGDL GKRMILRNLL EKHKDELRLF HRSAGQPGFA 

       130        140        150        160        170        180 
DALAGALSEL KLYNIDATSL DRCQTEVIER QGAGLLADKL HDLALLYGAL KTFLEGKYTD 

       190        200        210        220        230        240 
PDDYLHLLAQ RIPGSVMLRD AEVWVDGFAG FTPQELSVLA ALMSACRRVN VALCLDPTEL 

       250        260        270        280        290        300 
DEPCDLDDPF FRTRETQAKL LQLAFDRGIV IEKALHLVPA PGQSAPRFQH PELQHLESFY 

       310        320        330        340        350        360 
FRRPAPPWLG GTQSLFVCAA ANRRSEVEGA AREIIRLCRD QGYRWRDVAV LLRDLESYYD 

       370        380        390        400        410        420 
SIESVFSDFG IPLFIDSKRP APHHPLVELI RSALEAVLRD WAYEPVFRYL KTDLVPLSRG 

       430        440        450        460        470        480 
ETDRLENYVL AHGIRGRRWR DREPWLFRRR LTLGEDALSE FSDQELAELA EINGARDRAR 

       490        500        510        520        530        540 
AALASFHKAV VGAKAVRPIT AALYQLLVDL KAPEQISCWI AEAEAAGRIE EAHAHRQVWA 

       550        560        570        580        590        600 
AVIDLFDQIV ESLGETPLDL ATYAVVMEAG LDSLKLSLIP PELDQVFVGS LDRSRCPGIR 

       610        620        630        640        650        660 
AAFVLGVSEG VLPARPKDDG IFDDKERERI YALTGLELAP GSRRRLFDEE YHIYVALTRA 

       670        680        690        700        710        720 
SERCYLSYPL ADAEGRAQLP SSVIARVREL FPQVTERTWL LEPGSLPTGA GLSGSAFAGA 

       730        740        750        760        770        780 
ATAETAAMAQ TALAARTTKE AAMSAVEREL AEEYIVHPAR SLSYLIPSLR EALARRPVPL 

       790        800        810        820        830        840 
VWWAVYSWLA ERPAWRELLA RVLSGLWHKN REQDLPKPVS RRLFGDPLRA SVSRIERFLA 

       850        860        870        880        890        900 
CPFSHFISHG LRLKERRIFR LAAPDLGEFF HAALKQFGER LRRDRLDWGA LPREELFRLT 

       910        920        930        940        950        960 
GEVVSDLAPQ LQNEILLSTA RHRYLTGRLQ QTLSRAVIVL GEHARRGQFR PLALEVGFGP 

       970        980        990       1000       1010       1020 
EELLPPVQVP LTGGRWMDLV GRIDRIDGFF VGDASWLRVI DYKSNRANLK LADIAHGLKL 

      1030       1040       1050       1060       1070       1080 
QLLAYLDVAL RHGNRLLQGC ALGEDRPSSL SAATACHPGG MLYFGICDPL LSTGGPLTRD 

      1090       1100       1110       1120       1130       1140 
EAERAILKEA KMCGHVLADP AVVAAMDGQT RQGYSPLIPV GLKKEGGFYS ASSVLTMEQF 

      1150       1160       1170       1180       1190       1200 
ALLRQYLQSI VARVGEAILD GLVSIRPHRR DDRLACSFCP YKSVCQFDLL LQDNDYRLLI 

      1210       1220 
DDPPEKIWEK IAAGPKAIFE GVIR 

« Hide

References

[1]"The genome of Heliobacterium modesticaldum, a phototrophic representative of the Firmicutes containing the simplest photosynthetic apparatus."
Sattley W.M., Madigan M.T., Swingley W.D., Cheung P.C., Clocksin K.M., Conrad A.L., Dejesa L.C., Honchak B.M., Jung D.O., Karbach L.E., Kurdoglu A., Lahiri S., Mastrian S.D., Page L.E., Taylor H.L., Wang Z.T., Raymond J., Chen M., Blankenship R.E., Touchman J.W.
J. Bacteriol. 190:4687-4696(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 51547 / Ice1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000930 Genomic DNA. Translation: ABZ85506.1.
RefSeqYP_001681517.1. NC_010337.2.

3D structure databases

ProteinModelPortalB0TDI1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING498761.HM1_2997.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABZ85506; ABZ85506; HM1_2997.
GeneID5911241.
KEGGhmo:HM1_2997.
PATRIC22109967. VBIHelMod36755_2667.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG3857.
HOGENOMHOG000285805.
KOK16899.
OMAVPDQMTF.
OrthoDBEOG6DC6FN.

Enzyme and pathway databases

BioCycHMOD498761:GI46-3012-MONOMER.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
HAMAPMF_01452. AddB_type1.
InterProIPR014140. DNA_helicase_suAddB.
IPR014017. DNA_helicase_UvrD-like_C.
IPR027417. P-loop_NTPase.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
PROSITEPS51198. UVRD_HELICASE_ATP_BIND. 1 hit.
PS51217. UVRD_HELICASE_CTER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameADDB_HELMI
AccessionPrimary (citable) accession number: B0TDI1
Entry history
Integrated into UniProtKB/Swiss-Prot: July 7, 2009
Last sequence update: April 8, 2008
Last modified: May 14, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families