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Protein

ATP-dependent helicase/nuclease subunit A

Gene

addA

Organism
Heliobacterium modesticaldum (strain ATCC 51547 / Ice1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

The heterodimer acts as both an ATP-dependent DNA helicase and an ATP-dependent, dual-direction single-stranded exonuclease. Recognizes the chi site generating a DNA molecule suitable for the initiation of homologous recombination. The AddA nuclease domain is required for chi fragment generation; this subunit has the helicase and 3' -> 5' nuclease activities.UniRule annotation

Catalytic activityi

ATP + H2O = ADP + phosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi47 – 54ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Exonuclease, Helicase, Hydrolase, Nuclease
Biological processDNA damage, DNA repair
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent helicase/nuclease subunit AUniRule annotation (EC:3.1.-.-UniRule annotation, EC:3.6.4.12UniRule annotation)
Alternative name(s):
ATP-dependent helicase/nuclease AddAUniRule annotation
Gene namesi
Name:addAUniRule annotation
Ordered Locus Names:Helmi_28800
ORF Names:HM1_2996
OrganismiHeliobacterium modesticaldum (strain ATCC 51547 / Ice1)
Taxonomic identifieri498761 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesHeliobacteriaceaeHeliobacterium
Proteomesi
  • UP000008550 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003792741 – 1396ATP-dependent helicase/nuclease subunit AAdd BLAST1396

Interactioni

Subunit structurei

Heterodimer of AddA and AddB/RexB.UniRule annotation

Protein-protein interaction databases

STRINGi498761.HM1_2996

Structurei

3D structure databases

ProteinModelPortaliB0TDI0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini26 – 532UvrD-like helicase ATP-bindingUniRule annotationAdd BLAST507
Domaini615 – 920UvrD-like helicase C-terminalUniRule annotationAdd BLAST306

Sequence similaritiesi

Belongs to the helicase family. AddA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107R2I Bacteria
COG1074 LUCA
HOGENOMiHOG000015621
KOiK16898
OMAiKQSIYRW
OrthoDBiPOG091H004Y

Family and domain databases

Gene3Di3.90.320.10, 1 hit
HAMAPiMF_01451 AddA, 1 hit
InterProiView protein in InterPro
IPR014152 DNA_helicase_suAddA
IPR014017 DNA_helicase_UvrD-like_C
IPR000212 DNA_helicase_UvrD/REP
IPR011604 Exonuc_phg/RecB_C
IPR027417 P-loop_NTPase
IPR038726 PDDEXK_AddAB-type
IPR011335 Restrct_endonuc-II-like
IPR014016 UvrD-like_ATP-bd
IPR034739 UvrD/AddA_N
PANTHERiPTHR11070 PTHR11070, 1 hit
PTHR11070:SF14 PTHR11070:SF14, 1 hit
PfamiView protein in Pfam
PF12705 PDDEXK_1, 1 hit
PF00580 UvrD-helicase, 1 hit
PF13361 UvrD_C, 1 hit
SUPFAMiSSF52540 SSF52540, 6 hits
SSF52980 SSF52980, 1 hit
PROSITEiView protein in PROSITE
PS51198 UVRD_HELICASE_ATP_BIND, 1 hit
PS51217 UVRD_HELICASE_CTER, 1 hit

Sequencei

Sequence statusi: Complete.

B0TDI0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNREALCHDD PIGHDRLRPD SIPRDPKWTD EQWQAITARN SDILVAAAAG
60 70 80 90 100
AGKTAVLVER LIGIIKEGVD VDRLLVVTFT NAAAAEMRER IRTALTKELA
110 120 130 140 150
RHPHQTWLRQ QLVLLNRATI TTLHSFCLDL VRKYYYRLDL DPAFRVADET
160 170 180 190 200
EIALLRQDVL DEVFERFYER AGAKEGTESK GEYFTALVDA YGGDRDDSPL
210 220 230 240 250
QDIVLQLYEK ALSQPWPEQW LQDILGKFQE AGDTIAEQTL PGELPAWEAL
260 270 280 290 300
PWFAALREEM SIDLAEVESL LVRALSLCRQ PGGPAAYGDA VTADLQLTQD
310 320 330 340 350
LHLAAGRSWT QLHECFQALS FTRVKAVRGP VDESLKKEVS RLRYQAKKKL
360 370 380 390 400
TDLQKKYFLR TPHDLVSDLT QVLPLMATLV DVVLAFDRAF QAAKREKRLV
410 420 430 440 450
DFNDLEHFCL RLLLDESASP GVPVPSSLAL ELKEHFAEVL TDEYQDTNTV
460 470 480 490 500
QETILTLLSR NNRFMVGDVK QSIYRFRLAE PNLFLEKYRS FSPYDTPGPV
510 520 530 540 550
NSDHGAPDHA FSGQGASVPG ARIDLAKNFR SRRNVLSAVN DLFRRIMTPR
560 570 580 590 600
AGEMAYDRQA ELVYGAAFPE LPGRSGDPVI ELWLLQRQPD ADGSEESIET
610 620 630 640 650
QQNANETKGA IDGDHKNIAK AGESPAQNTA DVAGESPSDD AGEDAGQPTG
660 670 680 690 700
GEEELLLLET AQYEARLAAR RIEELMKSAM PVYDREQGGY RPVCYRDIVI
710 720 730 740 750
LMRSTKGRAD IFLEEFRAAA IPAYADTGAG YFEATEVSIL LSLLRVIDNP
760 770 780 790 800
CQDIPLAAVL RSPIFRFTGE DLARIRLAEP RKTFFDAIEA FIGQAEAAAR
810 820 830 840 850
DTFTPIDPVA IRLREFLRQI DAWRTLARRD SLAKLIATIY RETGFYDYAG
860 870 880 890 900
AMPGGGQRQA NLRALYDRAR QYEATTLRGL FRFLRFIERL QDQGGDLGTA
910 920 930 940 950
RTLGEKEDVV RIMSIHKSKG LEFPVVFVAG LGSQFNLQDL RRDLLIHKNL
960 970 980 990 1000
GLGPVVVDTQ LRYRYPTVAK LAIQRRLHRE TLAEEMRILY VALTRAKEKL
1010 1020 1030 1040 1050
ILLGTVREVA KSAQQWLHDA EGIPVAAALP DEVLLRAKCY LDWIGPALVG
1060 1070 1080 1090 1100
HPDGQEIRRW AEGAPALGAT LPENNLTMTK NETMTEQERI DGSSRWRLFY
1110 1120 1130 1140 1150
AAKKELIGLR KETGSETPFN PVWLEKLRRL EPIIPTDLVD ASLSWSYPYT
1160 1170 1180 1190 1200
GLSGIAAKVA VSRIKKQFVW HSPETPPSSE TPPSLEIPPS LETPPSLETQ
1210 1220 1230 1240 1250
TPSPDALVDL RPRFLQQDRR LTATERGSAV HLFLQHLDLA GEISQAGIAG
1260 1270 1280 1290 1300
QADRLVKLEL LSLEQRQALN EEEILRFLES PLGHRLRKAK QVMREVPFTL
1310 1320 1330 1340 1350
ALPAAEIYPE KRAEGESVII QGVIDCLFEE DDGWLLLDYK TDRCPVGMDR
1360 1370 1380 1390
ERWLQQLRDS YLGQVNLYHR AVESVLKVKV KGRCLFLLSI GREMAL
Length:1,396
Mass (Da):156,971
Last modified:April 8, 2008 - v1
Checksum:i4E0C4CCEE9888B11
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000930 Genomic DNA Translation: ABZ85505.1

Genome annotation databases

EnsemblBacteriaiABZ85505; ABZ85505; HM1_2996
KEGGihmo:HM1_2996

Entry informationi

Entry nameiADDA_HELMI
AccessioniPrimary (citable) accession number: B0TDI0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 7, 2009
Last sequence update: April 8, 2008
Last modified: April 25, 2018
This is version 64 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health