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Protein

ATP-dependent helicase/nuclease subunit A

Gene

addA

Organism
Heliobacterium modesticaldum (strain ATCC 51547 / Ice1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

The heterodimer acts as both an ATP-dependent DNA helicase and an ATP-dependent, dual-direction single-stranded exonuclease. Recognizes the chi site generating a DNA molecule suitable for the initiation of homologous recombination. The AddA nuclease domain is required for chi fragment generation; this subunit has the helicase and 3' -> 5' nuclease activities.UniRule annotation

Catalytic activityi

ATP + H2O = ADP + phosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi47 – 548ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Exonuclease, Helicase, Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciHMOD498761:GI46-3011-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent helicase/nuclease subunit AUniRule annotation (EC:3.1.-.-UniRule annotation, EC:3.6.4.12UniRule annotation)
Alternative name(s):
ATP-dependent helicase/nuclease AddAUniRule annotation
Gene namesi
Name:addAUniRule annotation
Ordered Locus Names:Helmi_28800
ORF Names:HM1_2996
OrganismiHeliobacterium modesticaldum (strain ATCC 51547 / Ice1)
Taxonomic identifieri498761 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesHeliobacteriaceaeHeliobacterium
Proteomesi
  • UP000008550 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13961396ATP-dependent helicase/nuclease subunit APRO_0000379274Add
BLAST

Interactioni

Subunit structurei

Heterodimer of AddA and AddB/RexB.UniRule annotation

Protein-protein interaction databases

STRINGi498761.HM1_2996.

Structurei

3D structure databases

ProteinModelPortaliB0TDI0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini26 – 532507UvrD-like helicase ATP-bindingUniRule annotationAdd
BLAST
Domaini615 – 920306UvrD-like helicase C-terminalUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the helicase family. AddA subfamily.UniRule annotation
Contains 1 uvrD-like helicase ATP-binding domain.UniRule annotation
Contains 1 uvrD-like helicase C-terminal domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107R2I. Bacteria.
COG1074. LUCA.
HOGENOMiHOG000015621.
KOiK16898.
OMAiIDNPYQD.
OrthoDBiEOG6HJ22G.

Family and domain databases

Gene3Di3.40.50.300. 5 hits.
3.90.320.10. 1 hit.
HAMAPiMF_01451. AddA.
InterProiIPR014152. DNA_helicase_suAddA.
IPR014017. DNA_helicase_UvrD-like_C.
IPR000212. DNA_helicase_UvrD/REP.
IPR011604. Exonuc_phg/RecB_C.
IPR027417. P-loop_NTPase.
IPR011335. Restrct_endonuc-II-like.
IPR014016. UvrD-like_ATP-bd.
[Graphical view]
PANTHERiPTHR11070. PTHR11070. 6 hits.
PfamiPF00580. UvrD-helicase. 1 hit.
PF13361. UvrD_C. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 6 hits.
SSF52980. SSF52980. 1 hit.
PROSITEiPS51198. UVRD_HELICASE_ATP_BIND. 1 hit.
PS51217. UVRD_HELICASE_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B0TDI0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNREALCHDD PIGHDRLRPD SIPRDPKWTD EQWQAITARN SDILVAAAAG
60 70 80 90 100
AGKTAVLVER LIGIIKEGVD VDRLLVVTFT NAAAAEMRER IRTALTKELA
110 120 130 140 150
RHPHQTWLRQ QLVLLNRATI TTLHSFCLDL VRKYYYRLDL DPAFRVADET
160 170 180 190 200
EIALLRQDVL DEVFERFYER AGAKEGTESK GEYFTALVDA YGGDRDDSPL
210 220 230 240 250
QDIVLQLYEK ALSQPWPEQW LQDILGKFQE AGDTIAEQTL PGELPAWEAL
260 270 280 290 300
PWFAALREEM SIDLAEVESL LVRALSLCRQ PGGPAAYGDA VTADLQLTQD
310 320 330 340 350
LHLAAGRSWT QLHECFQALS FTRVKAVRGP VDESLKKEVS RLRYQAKKKL
360 370 380 390 400
TDLQKKYFLR TPHDLVSDLT QVLPLMATLV DVVLAFDRAF QAAKREKRLV
410 420 430 440 450
DFNDLEHFCL RLLLDESASP GVPVPSSLAL ELKEHFAEVL TDEYQDTNTV
460 470 480 490 500
QETILTLLSR NNRFMVGDVK QSIYRFRLAE PNLFLEKYRS FSPYDTPGPV
510 520 530 540 550
NSDHGAPDHA FSGQGASVPG ARIDLAKNFR SRRNVLSAVN DLFRRIMTPR
560 570 580 590 600
AGEMAYDRQA ELVYGAAFPE LPGRSGDPVI ELWLLQRQPD ADGSEESIET
610 620 630 640 650
QQNANETKGA IDGDHKNIAK AGESPAQNTA DVAGESPSDD AGEDAGQPTG
660 670 680 690 700
GEEELLLLET AQYEARLAAR RIEELMKSAM PVYDREQGGY RPVCYRDIVI
710 720 730 740 750
LMRSTKGRAD IFLEEFRAAA IPAYADTGAG YFEATEVSIL LSLLRVIDNP
760 770 780 790 800
CQDIPLAAVL RSPIFRFTGE DLARIRLAEP RKTFFDAIEA FIGQAEAAAR
810 820 830 840 850
DTFTPIDPVA IRLREFLRQI DAWRTLARRD SLAKLIATIY RETGFYDYAG
860 870 880 890 900
AMPGGGQRQA NLRALYDRAR QYEATTLRGL FRFLRFIERL QDQGGDLGTA
910 920 930 940 950
RTLGEKEDVV RIMSIHKSKG LEFPVVFVAG LGSQFNLQDL RRDLLIHKNL
960 970 980 990 1000
GLGPVVVDTQ LRYRYPTVAK LAIQRRLHRE TLAEEMRILY VALTRAKEKL
1010 1020 1030 1040 1050
ILLGTVREVA KSAQQWLHDA EGIPVAAALP DEVLLRAKCY LDWIGPALVG
1060 1070 1080 1090 1100
HPDGQEIRRW AEGAPALGAT LPENNLTMTK NETMTEQERI DGSSRWRLFY
1110 1120 1130 1140 1150
AAKKELIGLR KETGSETPFN PVWLEKLRRL EPIIPTDLVD ASLSWSYPYT
1160 1170 1180 1190 1200
GLSGIAAKVA VSRIKKQFVW HSPETPPSSE TPPSLEIPPS LETPPSLETQ
1210 1220 1230 1240 1250
TPSPDALVDL RPRFLQQDRR LTATERGSAV HLFLQHLDLA GEISQAGIAG
1260 1270 1280 1290 1300
QADRLVKLEL LSLEQRQALN EEEILRFLES PLGHRLRKAK QVMREVPFTL
1310 1320 1330 1340 1350
ALPAAEIYPE KRAEGESVII QGVIDCLFEE DDGWLLLDYK TDRCPVGMDR
1360 1370 1380 1390
ERWLQQLRDS YLGQVNLYHR AVESVLKVKV KGRCLFLLSI GREMAL
Length:1,396
Mass (Da):156,971
Last modified:April 8, 2008 - v1
Checksum:i4E0C4CCEE9888B11
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000930 Genomic DNA. Translation: ABZ85505.1.
RefSeqiWP_012283980.1. NC_010337.2.

Genome annotation databases

EnsemblBacteriaiABZ85505; ABZ85505; HM1_2996.
KEGGihmo:HM1_2996.
PATRICi22109965. VBIHelMod36755_2666.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000930 Genomic DNA. Translation: ABZ85505.1.
RefSeqiWP_012283980.1. NC_010337.2.

3D structure databases

ProteinModelPortaliB0TDI0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi498761.HM1_2996.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABZ85505; ABZ85505; HM1_2996.
KEGGihmo:HM1_2996.
PATRICi22109965. VBIHelMod36755_2666.

Phylogenomic databases

eggNOGiENOG4107R2I. Bacteria.
COG1074. LUCA.
HOGENOMiHOG000015621.
KOiK16898.
OMAiIDNPYQD.
OrthoDBiEOG6HJ22G.

Enzyme and pathway databases

BioCyciHMOD498761:GI46-3011-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 5 hits.
3.90.320.10. 1 hit.
HAMAPiMF_01451. AddA.
InterProiIPR014152. DNA_helicase_suAddA.
IPR014017. DNA_helicase_UvrD-like_C.
IPR000212. DNA_helicase_UvrD/REP.
IPR011604. Exonuc_phg/RecB_C.
IPR027417. P-loop_NTPase.
IPR011335. Restrct_endonuc-II-like.
IPR014016. UvrD-like_ATP-bd.
[Graphical view]
PANTHERiPTHR11070. PTHR11070. 6 hits.
PfamiPF00580. UvrD-helicase. 1 hit.
PF13361. UvrD_C. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 6 hits.
SSF52980. SSF52980. 1 hit.
PROSITEiPS51198. UVRD_HELICASE_ATP_BIND. 1 hit.
PS51217. UVRD_HELICASE_CTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome of Heliobacterium modesticaldum, a phototrophic representative of the Firmicutes containing the simplest photosynthetic apparatus."
    Sattley W.M., Madigan M.T., Swingley W.D., Cheung P.C., Clocksin K.M., Conrad A.L., Dejesa L.C., Honchak B.M., Jung D.O., Karbach L.E., Kurdoglu A., Lahiri S., Mastrian S.D., Page L.E., Taylor H.L., Wang Z.T., Raymond J., Chen M., Blankenship R.E., Touchman J.W.
    J. Bacteriol. 190:4687-4696(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 51547 / Ice1.

Entry informationi

Entry nameiADDA_HELMI
AccessioniPrimary (citable) accession number: B0TDI0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 7, 2009
Last sequence update: April 8, 2008
Last modified: December 9, 2015
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.