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Protein

ATP-dependent 6-phosphofructokinase

Gene

pfk

Organism
Heliobacterium modesticaldum (strain ATCC 51547 / Ice1)
Status
Unreviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei17 – 171ATP; via amide nitrogenUniRule annotation
Metal bindingi121 – 1211Magnesium; catalyticUniRule annotation
Sitei122 – 1221Important for substrate specificity; cannot use PPi as phosphoryl donorUniRule annotation
Active sitei145 – 1451Proton acceptorUniRule annotation
Binding sitei180 – 1801Substrate; shared with dimeric partnerUniRule annotation
Binding sitei240 – 2401SubstrateUniRule annotation
Binding sitei284 – 2841Substrate; shared with dimeric partnerUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi80 – 812ATPUniRule annotation
Nucleotide bindingi120 – 1234ATPUniRule annotation

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: InterPro
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

KinaseUniRule annotation, Transferase

Keywords - Biological processi

GlycolysisUniRule annotationSAAS annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciHMOD498761:GI46-103-MONOMER.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:pfkImported
Synonyms:pfkAUniRule annotation
ORF Names:HM1_0103Imported
OrganismiHeliobacterium modesticaldum (strain ATCC 51547 / Ice1)Imported
Taxonomic identifieri498761 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesHeliobacteriaceaeHeliobacterium
ProteomesiUP000008550 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Homodimer or homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi498761.HM1_0103.

Structurei

3D structure databases

ProteinModelPortaliB0TD30.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni143 – 1453Substrate bindingUniRule annotation
Regioni187 – 1893Substrate bindingUniRule annotation
Regioni290 – 2934Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. Mixed-substrate PFK group III subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0205.
HOGENOMiHOG000248869.
KOiK00850.
OMAiRGLYNND.
OrthoDBiEOG644ZRM.

Family and domain databases

HAMAPiMF_01976. Phosphofructokinase_III.
InterProiIPR022953. ATP_PFK.
IPR012003. ATP_PFK_prok-type.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
IPR012829. Phosphofructokinase_III.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02483. PFK_mixed. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B0TD30-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWMQGQIKRI GVLTGGGDCP GLNAVIRAVV KTAIRRYDLE VVGIIDGFYG
60 70 80 90 100
LVNDVMEPLT ESSVSGILHR GGTILGTTNR DNPFRYAVEK DGQLTFEDRS
110 120 130 140 150
EQCIVNMKKR GIDALVIIGG DGSLSIALEF HKKGIPVVGV PKTIDNDLSA
160 170 180 190 200
TDVTFGFDSA VTTATEAIDK LHTTAESHHR IMILEVMGRY AGWIALYSGL
210 220 230 240 250
AGGADVILIP EIPFDLGKVC DHIRARAERG RKFSIVVIAE GAKEIGGELV
260 270 280 290 300
VQKLVAQSHD PVRLGGVGHV VAQKLEMCTG METRVTVLGH VQRGGSPTAE
310 320 330 340 350
DRILSTRYGS AAVDAIMSGQ FGKMVALRTP KILPVPLEEA VGTAKQVNPD
360
GQIVQAAKSV GISFGD
Length:366
Mass (Da):39,021
Last modified:April 8, 2008 - v1
Checksum:iD1022F95CF765526
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000930 Genomic DNA. Translation: ABZ82728.1.
RefSeqiWP_012281277.1. NC_010337.2.
YP_001678739.1. NC_010337.2.

Genome annotation databases

EnsemblBacteriaiABZ82728; ABZ82728; HM1_0103.
KEGGihmo:HM1_0103.
PATRICi22104529. VBIHelMod36755_0083.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000930 Genomic DNA. Translation: ABZ82728.1.
RefSeqiWP_012281277.1. NC_010337.2.
YP_001678739.1. NC_010337.2.

3D structure databases

ProteinModelPortaliB0TD30.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi498761.HM1_0103.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABZ82728; ABZ82728; HM1_0103.
KEGGihmo:HM1_0103.
PATRICi22104529. VBIHelMod36755_0083.

Phylogenomic databases

eggNOGiCOG0205.
HOGENOMiHOG000248869.
KOiK00850.
OMAiRGLYNND.
OrthoDBiEOG644ZRM.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.
BioCyciHMOD498761:GI46-103-MONOMER.

Family and domain databases

HAMAPiMF_01976. Phosphofructokinase_III.
InterProiIPR022953. ATP_PFK.
IPR012003. ATP_PFK_prok-type.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
IPR012829. Phosphofructokinase_III.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02483. PFK_mixed. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome of Heliobacterium modesticaldum, a phototrophic representative of the Firmicutes containing the simplest photosynthetic apparatus."
    Sattley W.M., Madigan M.T., Swingley W.D., Cheung P.C., Clocksin K.M., Conrad A.L., Dejesa L.C., Honchak B.M., Jung D.O., Karbach L.E., Kurdoglu A., Lahiri S., Mastrian S.D., Page L.E., Taylor H.L., Wang Z.T., Raymond J., Chen M., Blankenship R.E., Touchman J.W.
    J. Bacteriol. 190:4687-4696(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 51547 / Ice1Imported.

Entry informationi

Entry nameiB0TD30_HELMI
AccessioniPrimary (citable) accession number: B0TD30
Entry historyi
Integrated into UniProtKB/TrEMBL: April 8, 2008
Last sequence update: April 8, 2008
Last modified: April 29, 2015
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.