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B0TBP1 (FTHS_HELMI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 41. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Formate--tetrahydrofolate ligase

EC=6.3.4.3
Alternative name(s):
Formyltetrahydrofolate synthetase
Short name=FHS
Short name=FTHFS
Gene names
Name:fhs
Ordered Locus Names:Helmi_12550
ORF Names:HM1_0672
OrganismHeliobacterium modesticaldum (strain ATCC 51547 / Ice1) [Complete proteome] [HAMAP]
Taxonomic identifier498761 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesHeliobacteriaceaeHeliobacterium

Protein attributes

Sequence length556 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. HAMAP-Rule MF_01543

Pathway

One-carbon metabolism; tetrahydrofolate interconversion. HAMAP-Rule MF_01543

Sequence similarities

Belongs to the formate--tetrahydrofolate ligase family.

Ontologies

Keywords
   Biological processOne-carbon metabolism
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processfolic acid-containing compound biosynthetic process

Inferred from electronic annotation. Source: InterPro

tetrahydrofolate interconversion

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

formate-tetrahydrofolate ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 556556Formate--tetrahydrofolate ligase HAMAP-Rule MF_01543
PRO_1000146684

Regions

Nucleotide binding65 – 728ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B0TBP1 [UniParc].

Last modified April 8, 2008. Version 1.
Checksum: 6220867911A8E37A

FASTA55659,530
        10         20         30         40         50         60 
MKTDIEIAQE ATLQPIMDVA RSLGIPENEV ELYGNYKAKI SLKAFERLKE RREGKLILVT 

        70         80         90        100        110        120 
AINPTPAGEG KTTTTVGLGD ALRRLGKKVV IALREPSLGP SFGVKGGAAG GGYAQIVPME 

       130        140        150        160        170        180 
DINLHFTGDF HAVTSAHNLL AAMLDNSLQQ GNPLNIDPRQ VVFRRVLDLN DRALRKVIVG 

       190        200        210        220        230        240 
LGGRTDGIPR ESGFDITVAS EIMAILCLSK DLMDLKARCA KIVVAYTYDG KPVTAADLEA 

       250        260        270        280        290        300 
QGSMAVLLKD AIKPNLVQTL EHTPAFVHGG PFANIAHGCN SVTATQLALK LGDYCVTEAG 

       310        320        330        340        350        360 
FGADLGAEKF FNLKCRLAGL KPDCTVIVAT VRALKSHGGV AKADLNRENL EALAKGFGNL 

       370        380        390        400        410        420 
EKHIENVAKF GVPAVVAINA FPTDTKAELD YVYERCRQMG IDVALSEVWA KGGEGGRELA 

       430        440        450        460        470        480 
EKVVAAIETK PSRFRVLYDS SLPIKEKIAA IVREVYGGDA VAYTPEAEKH IKTFTDLGYG 

       490        500        510        520        530        540 
ALPVCMAKTQ YSLSDDMTKI GRPEGFTVTV REVRLSAGAG FLVVITGAIM TMPGLPKRPA 

       550 
ACAIDIDENG RIVGLF 

« Hide

References

[1]"The genome of Heliobacterium modesticaldum, a phototrophic representative of the Firmicutes containing the simplest photosynthetic apparatus."
Sattley W.M., Madigan M.T., Swingley W.D., Cheung P.C., Clocksin K.M., Conrad A.L., Dejesa L.C., Honchak B.M., Jung D.O., Karbach L.E., Kurdoglu A., Lahiri S., Mastrian S.D., Page L.E., Taylor H.L., Wang Z.T., Raymond J., Chen M., Blankenship R.E., Touchman J.W.
J. Bacteriol. 190:4687-4696(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 51547 / Ice1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000930 Genomic DNA. Translation: ABZ83880.1.
RefSeqYP_001679891.1. NC_010337.2.

3D structure databases

ProteinModelPortalB0TBP1.
SMRB0TBP1. Positions 4-554.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING498761.HM1_0672.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABZ83880; ABZ83880; HM1_0672.
GeneID5911277.
KEGGhmo:HM1_0672.
PATRIC22106769. VBIHelMod36755_1164.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2759.
HOGENOMHOG000040280.
KOK01938.
OMACGEIMTM.
OrthoDBEOG6PCPSP.
ProtClustDBPRK13505.

Enzyme and pathway databases

BioCycHMOD498761:GI46-1297-MONOMER.
UniPathwayUPA00193.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
HAMAPMF_01543. FTHFS.
InterProIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
PROSITEPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameFTHS_HELMI
AccessionPrimary (citable) accession number: B0TBP1
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: April 8, 2008
Last modified: April 16, 2014
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways