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B0TBJ3 (SYL_HELMI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 48. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Helmi_25810
ORF Names:HM1_2677
OrganismHeliobacterium modesticaldum (strain ATCC 51547 / Ice1) [Complete proteome] [HAMAP]
Taxonomic identifier498761 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesHeliobacteriaceaeHeliobacterium

Protein attributes

Sequence length846 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 846846Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000091322

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif586 – 5905"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5891ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B0TBJ3 [UniParc].

Last modified April 8, 2008. Version 1.
Checksum: D4062D5A09098691

FASTA84696,367
        10         20         30         40         50         60 
MQERYDYQQI EKKWQKYWAD TGMFRLQQEE GRPKYYCLEM FPYPSGNLHM GHVRVYSIGD 

        70         80         90        100        110        120 
VVARFKTMQG YNVLHPMGWD AFGLPAENAA IKHKVPPADW TWSNIANMRR QLQSMGISYD 

       130        140        150        160        170        180 
WEREVATCHP DYYKWTQWLF LQLYKAGLAY KKKANVNWCP DCATVLANEQ VVDGLCERCD 

       190        200        210        220        230        240 
ATVEKKALEQ WFFRITDYAQ RLLDDLNKLP GWPDKVKTMQ ENWIGRSEGV EADFALENPV 

       250        260        270        280        290        300 
GGVDKLTVYT TRQDTIFGVT YMVLAPEHPA VEALVSGNPR EAELRAFIKK VLSQSEIDRT 

       310        320        330        340        350        360 
ANDTEKEGIF TGHYCINPLT GEKVAVYLAN YVLVDYGTGA VMGVPAHDQR DFEFATKYNI 

       370        380        390        400        410        420 
PMKVVIQPEG LPDLDVEEMD HAYEGEGRLV NSGEFDGLIN TQALDVIADK LEREGKGVRK 

       430        440        450        460        470        480 
VNYRLRDWLI SRQRYWGAPI PVIYCEKCGE VAVPDEQLPV MLPTDVAFTP SGESPLTSRP 

       490        500        510        520        530        540 
DFYECTCPRC GGKGRRETDT MDTFVCSSWY FLRYCTPKDK DHAFLQEDVD YWMNVDQYIG 

       550        560        570        580        590        600 
GVEHAILHLM YARFFTKVLY DLKLVKVEEP FENLLTQGMV LMGGSKMSKS KGNTVSPEEI 

       610        620        630        640        650        660 
IAKYGADTAR LFILFAAPPE RDLEWSDRGV EGAHRFLHRL WRLVYAYSDQ IASVPGTMVG 

       670        680        690        700        710        720 
YGKGQPNGSK GDNLFSLNSD DRELVRVAHY TVKKVTEDIG QRFNFNTAVS AIMEMVNYFY 

       730        740        750        760        770        780 
QYKDKVPAEQ QNIPLVKDAL ARLVLVLAPF APHICEELWQ VIGGGESVYK QPWPTYDEQA 

       790        800        810        820        830        840 
LVRDEVEVVV QINGKVREKL RVANGLDGEA IKAKVLEMDK VQALIAGKSV VKLITVPNKL 


VNIVVK 

« Hide

References

[1]"The genome of Heliobacterium modesticaldum, a phototrophic representative of the Firmicutes containing the simplest photosynthetic apparatus."
Sattley W.M., Madigan M.T., Swingley W.D., Cheung P.C., Clocksin K.M., Conrad A.L., Dejesa L.C., Honchak B.M., Jung D.O., Karbach L.E., Kurdoglu A., Lahiri S., Mastrian S.D., Page L.E., Taylor H.L., Wang Z.T., Raymond J., Chen M., Blankenship R.E., Touchman J.W.
J. Bacteriol. 190:4687-4696(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 51547 / Ice1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000930 Genomic DNA. Translation: ABZ85206.1.
RefSeqYP_001681217.1. NC_010337.2.

3D structure databases

ProteinModelPortalB0TBJ3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING498761.HM1_2677.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABZ85206; ABZ85206; HM1_2677.
GeneID5909903.
KEGGhmo:HM1_2677.
PATRIC22109393. VBIHelMod36755_2400.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAREKNDWE.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycHMOD498761:GI46-2692-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_HELMI
AccessionPrimary (citable) accession number: B0TBJ3
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: April 8, 2008
Last modified: April 16, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries