B0TB90 (ISPE_HELMI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Short name=CMK
EC=2.7.1.148

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	Helmi_11920
ORF Names:	HM1_0738

Organism

Heliobacterium modesticaldum (strain ATCC 51547 / Ice1) [Complete proteome] [HAMAP]

Taxonomic identifier

498761 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Heliobacteriaceae › Heliobacterium

Protein attributes

Sequence length	315 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 315	315	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061		PRO_1000092090
Regions
	Nucleotide binding	93 – 103	11	ATP Potential
Sites
	Active site	8	1	By similarity
	Active site	135	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B0TB90 [UniParc]. Last modified April 8, 2008. Version 1. Checksum: 27C76CB79804C976	FASTA	315	33,695
	10 20 30 40 50 60 MRLLAPAKIN LALDVLGRRA DGYHQVVMVM QTIALADTVT VAVNEGHGAI RLAGGTEEAP 70 80 90 100 110 120 PDADNLVYRA AQLVRETAGL SCGVDIDLEK VIPVAAGLAG GSSDAAATVK ALNRLFRLGW 130 140 150 160 170 180 SDREMETLLA RLGSDIPFLV RGGTALATGR GEIVHRLPPA PAFWVVLVKP PFGASTPKVY 190 200 210 220 230 240 KALGAPALPD PLPWPQAMKP ATTPPGTAAY RMIEALKTGD YGNVLEALGN DLEQVTLEWH 250 260 270 280 290 300 PVLKEIKVQL TRFGCDRALM SGSGPTILGF TASEATARSV AAAMEEQWGP QRYRVLIART 310 LEREEADEWN VDCCR

References

[1]

"The genome of Heliobacterium modesticaldum, a phototrophic representative of the Firmicutes containing the simplest photosynthetic apparatus." Sattley W.M., Madigan M.T., Swingley W.D., Cheung P.C., Clocksin K.M., Conrad A.L., Dejesa L.C., Honchak B.M., Jung D.O., Karbach L.E., Kurdoglu A., Lahiri S., Mastrian S.D., Page L.E., Taylor H.L., Wang Z.T., Raymond J., Chen M., Blankenship R.E., Touchman J.W. J. Bacteriol. 190:4687-4696(2008) [PubMed: 18441057] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51547 / Ice1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000930 Genomic DNA. Translation: ABZ83817.1.
RefSeq	YP_001679828.1. NC_010337.2.
3D structure databases
ProteinModelPortal	B0TB90.
ModBase	Search...
Protein-protein interaction databases
STRING	B0TB90.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5909717.
GenomeReviews	Gene locus Helmi_11920 in contig CP000930_GR.
KEGG	hmo:HM1_0738.
PATRIC	22106641. VBIHelMod36755_1103.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG734593.
OMA	GCIASEK.
Enzyme and pathway databases
BioCyc	HMOD498761:HM1_0738-MONOMER.
Family and domain databases
HAMAP	MF_00061. IspE. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00919.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00154. IspE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_HELMI

Accession

Primary (citable) accession number: B0TB90

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	April 8, 2008
Last modified:	January 25, 2012
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program