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B0TAZ6 (DDL_HELMI) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:Helmi_24820
ORF Names:HM1_2570
OrganismHeliobacterium modesticaldum (strain ATCC 51547 / Ice1) [Complete proteome] [HAMAP]
Taxonomic identifier498761 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesHeliobacteriaceaeHeliobacterium

Protein attributes

Sequence length366 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 366366D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_0000341109

Regions

Domain140 – 346207ATP-grasp
Nucleotide binding173 – 22856ATP By similarity

Sites

Metal binding2991Magnesium or manganese 1 By similarity
Metal binding3131Magnesium or manganese 1 By similarity
Metal binding3131Magnesium or manganese 2 By similarity
Metal binding3151Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
B0TAZ6 [UniParc].

Last modified April 8, 2008. Version 1.
Checksum: 5323585A634C9953

FASTA36640,306
        10         20         30         40         50         60 
MMKQTIAVIC GGRSGEHEVS LTSAQSIVDA LDRFRFNVLT IGIDRHGAWW LGSNVIDNLR 

        70         80         90        100        110        120 
KGQQPYGQRV YFIPDPTCPG LIEENPVTRK PVPVDVFFPV LHGPNGEDGT IQGLFEAANV 

       130        140        150        160        170        180 
PYVGCGVLAS ACGMDKAIMK ALFAQSDLPQ LPYLVVLRNR WESAREQVID EVEDRLGYPC 

       190        200        210        220        230        240 
FVKPANMGSS VGISKATNRA ELVAAFDDAV RYDRKLIVEK GINVREIEVS VLGNDEVVAS 

       250        260        270        280        290        300 
VPGEIVPAHE FYDYDAKYAA ADSRLLIPAP IAAADVATFQ EMAIRAFRAI DGSGLSRVDF 

       310        320        330        340        350        360 
LLDKNSGEVY INEINTLPGF TSISMYPKLW EATGIPYGEL LSRLVDLGLA RYREKQRNAT 


ERLPRL 

« Hide

References

[1]"The genome of Heliobacterium modesticaldum, a phototrophic representative of the Firmicutes containing the simplest photosynthetic apparatus."
Sattley W.M., Madigan M.T., Swingley W.D., Cheung P.C., Clocksin K.M., Conrad A.L., Dejesa L.C., Honchak B.M., Jung D.O., Karbach L.E., Kurdoglu A., Lahiri S., Mastrian S.D., Page L.E., Taylor H.L., Wang Z.T., Raymond J., Chen M., Blankenship R.E., Touchman J.W.
J. Bacteriol. 190:4687-4696(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 51547 / Ice1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000930 Genomic DNA. Translation: ABZ85107.1.
RefSeqYP_001681118.1. NC_010337.2.

3D structure databases

ProteinModelPortalB0TAZ6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING498761.HM1_2570.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABZ85107; ABZ85107; HM1_2570.
GeneID5911953.
KEGGhmo:HM1_2570.
PATRIC22109195. VBIHelMod36755_2308.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011593.
KOK01921.
OMAMDKIAMK.
OrthoDBEOG64BQ73.
ProtClustDBPRK01966.

Enzyme and pathway databases

BioCycHMOD498761:GI46-2585-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_HELMI
AccessionPrimary (citable) accession number: B0TAZ6
Entry history
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: April 8, 2008
Last modified: February 19, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways