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B0TAB5 (MNMG_HELMI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 41. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG
Alternative name(s):
Glucose-inhibited division protein A
Gene names
Name:mnmG
Synonyms:gidA
Ordered Locus Names:Helmi_10270
ORF Names:HM1_0913
OrganismHeliobacterium modesticaldum (strain ATCC 51547 / Ice1) [Complete proteome] [HAMAP]
Taxonomic identifier498761 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesHeliobacteriaceaeHeliobacterium

Protein attributes

Sequence length637 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm5s2U34 By similarity. HAMAP-Rule MF_00129

Cofactor

FAD By similarity. HAMAP-Rule MF_00129

Subunit structure

Homodimer By similarity. Heterotetramer of two MnmE and two MnmG subunits By similarity. HAMAP-Rule MF_00129

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00129.

Sequence similarities

Belongs to the MnmG family.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentCytoplasm
   LigandFAD
Flavoprotein
NAD
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processtRNA wobble uridine modification

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionflavin adenine dinucleotide binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 637637tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG HAMAP-Rule MF_00129
PRO_0000345279

Regions

Nucleotide binding15 – 206FAD By similarity
Nucleotide binding274 – 28815NAD Potential

Sites

Binding site1271FAD; via amide nitrogen and carbonyl oxygen By similarity
Binding site1821FAD By similarity
Binding site3711FAD By similarity

Sequences

Sequence LengthMass (Da)Tools
B0TAB5 [UniParc].

Last modified April 8, 2008. Version 1.
Checksum: 8F06F9D4DB674263

FASTA63770,779
        10         20         30         40         50         60 
MTEYVAGKYD VIVIGAGHAG CEAALASARL GAKTLILTIS WDNVALMPCN PAIGGPAKGH 

        70         80         90        100        110        120 
LVREIDALGG QMGLTIDKTC IQIRLLNTGK GPAVHALRAQ ADKKRYQREM IRVLEHQENL 

       130        140        150        160        170        180 
DVRQAMVESV VVEQGRVRGI RTNTGAFFAA PALVITTGTY LRGRIIIGDL HYPGGPNGYF 

       190        200        210        220        230        240 
PSVALAASLR DIGVRLGRFK TGTPARVDGR TVDFSQMVEQ PGDREPLNFS FLSPRIERKQ 

       250        260        270        280        290        300 
ISCWLTYTTE ETHQIIRDNL HRSPLYAGVI EGTGPRYCPS IEDKVVRFAD KKSHQVFIEP 

       310        320        330        340        350        360 
EGEGTHEMYV QGMSTSLPED VQVTMLRSII GMKDVKIIRP GYAIEYDYVD PTQLRLSLEH 

       370        380        390        400        410        420 
QEIGGLFTAG QINGTSGYEE AAAQGLMAGI NAARFVKGEE PLVLKRSDAY IGVLIDDLVT 

       430        440        450        460        470        480 
KGTNEPYRML TSRAEYRLLL RQDNADQRLT EIGRSIGLVD DRRYRRFREK MQLLEQEVVR 

       490        500        510        520        530        540 
WKSSFVTPGN KKIQAILDEK KSAPLTKGVS LYDLLRRPEL TYDDLIPLCN DGEKDLPIDP 

       550        560        570        580        590        600 
EVAEQVEISA KFEGYLVKQQ AQVDRFNKLE NKRLPADLDY KRVHGLSNEG RQKLIARKPV 

       610        620        630 
SIGQASRISG VSPADISILL VYLEQQRRLA PGEGGVD 

« Hide

References

[1]"The genome of Heliobacterium modesticaldum, a phototrophic representative of the Firmicutes containing the simplest photosynthetic apparatus."
Sattley W.M., Madigan M.T., Swingley W.D., Cheung P.C., Clocksin K.M., Conrad A.L., Dejesa L.C., Honchak B.M., Jung D.O., Karbach L.E., Kurdoglu A., Lahiri S., Mastrian S.D., Page L.E., Taylor H.L., Wang Z.T., Raymond J., Chen M., Blankenship R.E., Touchman J.W.
J. Bacteriol. 190:4687-4696(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 51547 / Ice1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000930 Genomic DNA. Translation: ABZ83652.1.
RefSeqYP_001679663.1. NC_010337.2.

3D structure databases

ProteinModelPortalB0TAB5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING498761.HM1_0913.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABZ83652; ABZ83652; HM1_0913.
GeneID5909689.
KEGGhmo:HM1_0913.
PATRIC22106309. VBIHelMod36755_0947.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0445.
HOGENOMHOG000201060.
KOK03495.
OMAFHIEREQ.
OrthoDBEOG6W9X6J.

Enzyme and pathway databases

BioCycHMOD498761:GI46-1056-MONOMER.

Family and domain databases

HAMAPMF_00129. MnmG_GidA.
InterProIPR004416. GidA.
IPR026904. GidA-assoc_3.
IPR002218. GIDA-rel.
IPR020595. GIDA-rel_CS.
[Graphical view]
PfamPF01134. GIDA. 1 hit.
PF13932. GIDA_assoc_3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00136. gidA. 1 hit.
PROSITEPS01280. GIDA_1. 1 hit.
PS01281. GIDA_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMNMG_HELMI
AccessionPrimary (citable) accession number: B0TAB5
Entry history
Integrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: April 8, 2008
Last modified: May 14, 2014
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families