Reviewed, UniProtKB/Swiss-Prot B0T8W6 (QUEF_CAUSK)

Names and origin

Protein names

Recommended name:
    NADPH-dependent 7-cyano-7-deazaguanine reductase
    EC=1.7.1.13
Alternative name(s):
    7-cyano-7-carbaguanine reductase
    PreQ(0) reductase
    NADPH-dependent nitrile oxidoreductase

Gene names

Name:	queF
Ordered Locus Names:	Caul_3756

Organism

Caulobacter sp. (strain K31) [Complete proteome] [HAMAP]

Taxonomic identifier

366602 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Caulobacterales › Caulobacteraceae › Caulobacter

Protein attributes

Sequence length	151 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) By similarity.
Catalytic activity	7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH. HAMAP MF_00818
Pathway	tRNA modification; queuosine-tRNA biosynthesis. HAMAP MF_00818
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily.

Ontologies

Keywords
Biological process	Queuosine biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW queuosine biosynthetic process Inferred from electronic annotation. Source: HAMAP tetrahydrofolate biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP cyclohydrolase I activity Inferred from electronic annotation. Source: InterPro oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor Inferred from electronic annotation. Source: HAMAP queuine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 151	151	NADPH-dependent 7-cyano-7-deazaguanine reductase HAMAP MF_00818		PRO_1000083836

Sequences

	Sequence		Length	Mass (Da)
	B0T8W6-1 [UniParc]. Last modified April 8, 2008. Version 1. Checksum: 32104A7F0C406CC0	FASTA	151	16,831
	10 20 30 40 50 60 MTELHVTQLG QVVEPAASPD QAVLERVPNP QSDVTYLARF VAPEFTSLCP VTGQPDFAHL 70 80 90 100 110 120 VIDYAPGDWL IESKSLKLYL TSFRSHGSFH EDCTVKIGRK IVEIAQPRWL RIGGYWYPRG 130 140 150 GIPIDVFWQT GPAPEGLWVP DQGVAPYRGR G

References

[1]

"Complete sequence of chromosome of Caulobacter sp. K31." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Bruce D., Goodwin L., Thompson L.S., Brettin T., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Kim E., Stephens C., Richardson P. Submitted (JAN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000927 Genomic DNA. Translation: ABZ72883.1.
RefSeq	YP_001685381.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5901218.
GenomeReviews	Gene locus Caul_3756 in contig CP000927_GR.
KEGG	cak:Caul_3756.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	B0T8W6. HESCINT.
Family and domain databases
HAMAP	MF_00818. [Tree]
InterPro	IPR016856. CN_OxRdtase_NADPH-dep_QueF. IPR001474. GTP_CycHdrlase_I. [Graphical view]
Pfam	PF01227. GTP_cyclohydroI. 1 hit. [Graphical view]
PIRSF	PIRSF027377. Nitrile_oxidored_QueF. 1 hit.
TIGRFAMs	TIGR03139. QueF-II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

QUEF_CAUSK

Accession

Primary (citable) accession number: B0T8W6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	April 8, 2008
Last modified:	June 16, 2009
This is version 10 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)