B0T833 (MURD_CAUSK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-N-acetylmuramoylalanine--D-glutamate ligase
EC=6.3.2.9

Alternative name(s):
D-glutamic acid-adding enzyme
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Gene names

Name:	murD
Ordered Locus Names:	Caul_3669

Organism

Caulobacter sp. (strain K31) [Complete proteome] [HAMAP]

Taxonomic identifier

366602 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Caulobacterales › Caulobacteraceae › Caulobacter

Protein attributes

Sequence length	469 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) By similarity. HAMAP MF_00639
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanine + glutamate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate. HAMAP MF_00639
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00639
Subcellular location	Cytoplasm By similarity HAMAP MF_00639.
Sequence similarities	Belongs to the MurCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW UDP-N-acetylmuramoylalanine-D-glutamate ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 469	469	UDP-N-acetylmuramoylalanine--D-glutamate ligase HAMAP MF_00639		PRO_1000082677
Regions
	Nucleotide binding	123 – 129	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	B0T833 [UniParc]. Last modified April 8, 2008. Version 1. Checksum: 4A345F7E724C06F7	FASTA	469	49,294
	10 20 30 40 50 60 MIPVRGFEGK TVAVFGLGRT GLTAARALIA GGAKVALWDE KPESRQAAVA EGLNVVDLTT 70 80 90 100 110 120 SDWSDYAALM LSPGVPLTHP KPHWTVGKAK AAGVEVLGDI ELFARTVNAA PEHKKPKIIA 130 140 150 160 170 180 ITGTNGKSTT TALIGHLCRQ AGRDTRVGGN IGEGVLGLED MHGGAVYVLE LSSYQLDLTS 190 200 210 220 230 240 SLKPDAVVLL NISPDHLDRH GGMDGYIAAK RRIFLNQGKG DTAIIGVDDP WCQQICTEIT 250 260 270 280 290 300 AANRRTIWPI SAGKAMGRGV YALQGVLYDA TGERVTEMAD LLRARSLPGR HNWQNAAAAY 310 320 330 340 350 360 AAAKAIGIPA HQAVDGLMSF PGLAHRMETV GKLGKVRFVN DSKATNADAA RQAMSSYPKF 370 380 390 400 410 420 YWIAGGVPKA GGIDDLVDLF PRVAGAYLIG QAAEDFGKTL EGKAPARQCG DIETAVAAAY 430 440 450 460 ADAVASGEEA VVLLSPACAS FDQFADFEQR GEAFRAAVNG LGKPAAKRA

References

[1]

"Complete sequence of chromosome of Caulobacter sp. K31." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Bruce D., Goodwin L., Thompson L.S., Brettin T., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Kim E., Stephens C., Richardson P. Submitted (JAN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K31.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000927 Genomic DNA. Translation: ABZ72796.1.
RefSeq	YP_001685294.1. NC_010338.1.
3D structure databases
ProteinModelPortal	B0T833.
ModBase	Search...
Protein-protein interaction databases
STRING	B0T833.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5901124.
GenomeReviews	Gene locus Caul_3669 in contig CP000927_GR.
KEGG	cak:Caul_3669.
PATRIC	21320472. VBICauSp18104_4074.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG750024.
OMA	ACASWDM.
ProtClustDB	PRK01390.
Enzyme and pathway databases
BioCyc	CSP78:CAUL_3669-MONOMER.
Family and domain databases
HAMAP	MF_00639. MurD. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR016040. NAD(P)-bd_dom. IPR005762. UDP-N-AcMur-Glu_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K01925.
PANTHER	PTHR23135:SF2. PTHR23135:SF2. 1 hit.
Pfam	PF08245. Mur_ligase_M. 1 hit. [Graphical view]
SUPFAM	SSF53244. Mur_ligase_C. 1 hit. SSF53623. Mur_ligase_cen. 1 hit.
TIGRFAMs	TIGR01087. MurD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURD_CAUSK

Accession

Primary (citable) accession number: B0T833

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	April 8, 2008
Last modified:	January 25, 2012
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program