B0T7V9 (ISPE_CAUSK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Short name=CMK
EC=2.7.1.148

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	Caul_2169

Organism

Caulobacter sp. (strain K31) [Complete proteome] [HAMAP]

Taxonomic identifier

366602 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Caulobacterales › Caulobacteraceae › Caulobacter

Protein attributes

Sequence length	293 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 293	293	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061		PRO_0000335704
Regions
	Nucleotide binding	96 – 106	11	ATP Potential
Sites
	Active site	10	1	By similarity
	Active site	138	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B0T7V9 [UniParc]. Last modified April 8, 2008. Version 1. Checksum: 4D28711F35243AB6	FASTA	293	30,343
	10 20 30 40 50 60 MRLDAFAPAK VNLFLHVGGP DAAGYHPISS LMLFADVGDR VSLQAADAPS FEATGWFGAE 70 80 90 100 110 120 VPVDDGNLVV RAEMALRARL GGPTPPFRLI LDKALPIAAG LGGGSSDAGA ALRLLREALA 130 140 150 160 170 180 PDLSDADLEA VAGGLGADGA ACLWGAPVMA RGRGERLSPA PALPALHAVL VNPLVPSPTG 190 200 210 220 230 240 AVYRAYDAAV APEGEAPPPM LDGLESIEEV CAWLAGFTRN DLQAPAVALE PRIGQVLDLL 250 260 270 280 290 ADEPETLLAR MSGSGATCFA LCAGDIEAEG LAERIEQMRP DWWVKRCRLG GPF

References

[1]

"Complete sequence of chromosome of Caulobacter sp. K31." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Bruce D., Goodwin L., Thompson L.S., Brettin T., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Kim E., Stephens C., Richardson P. Submitted (JAN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K31.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000927 Genomic DNA. Translation: ABZ71297.1.
RefSeq	YP_001683795.1. NC_010338.1.
3D structure databases
ProteinModelPortal	B0T7V9.
ModBase	Search...
Protein-protein interaction databases
STRING	B0T7V9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5899624.
GenomeReviews	Gene locus Caul_2169 in contig CP000927_GR.
KEGG	cak:Caul_2169.
PATRIC	21317394. VBICauSp18104_2555.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG734593.
OMA	KINLHLG.
ProtClustDB	PRK14608.
Enzyme and pathway databases
BioCyc	CSP78:CAUL_2169-MONOMER.
Family and domain databases
HAMAP	MF_00061. IspE. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00919.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_CAUSK

Accession

Primary (citable) accession number: B0T7V9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	April 8, 2008
Last modified:	January 25, 2012
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program