B0T369 (RNPH_CAUSK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease PH
Short name=RNase PH
EC=2.7.7.56

Alternative name(s):
tRNA nucleotidyltransferase

Gene names

Name:	rph
Ordered Locus Names:	Caul_0167

Organism

Caulobacter sp. (strain K31) [Complete proteome] [HAMAP]

Taxonomic identifier

366602 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Caulobacterales › Caulobacteraceae › Caulobacter

Protein attributes

Sequence length	242 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates By similarity. HAMAP MF_00564
Catalytic activity	tRNA(n+1) + phosphate = tRNA(n) + a nucleoside diphosphate. HAMAP MF_00564
Sequence similarities	Belongs to the RNase PH family.

Ontologies

Keywords
Biological process	tRNA processing
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	tRNA binding Inferred from electronic annotation. Source: InterPro tRNA nucleotidyltransferase activity Inferred from electronic annotation. Source: EC tRNA-specific ribonuclease activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 242	242	Ribonuclease PH HAMAP MF_00564		PRO_1000082285

Sequences

	Sequence		Length	Mass (Da)
	B0T369 [UniParc]. Last modified April 8, 2008. Version 1. Checksum: 216686AE25FD43BE	FASTA	242	26,244
	10 20 30 40 50 60 MRPSERAPDV LRVVTLETGV NRYAEGSCLI SFGHTKVLVT ATVEENVPGW MRNKGAGWVT 70 80 90 100 110 120 AEYGMLPRAT HTRGRREAAL GKQSGRTQEI QRLIGRSLRA VVDLKALGER QISLDCDVLQ 130 140 150 160 170 180 ADGGTRTAAI TGAWVALRIA VNYLLEEGVL KTDPIVGQVA AVSCGVFKDT PVLDLDYEED 190 200 210 220 230 240 SQAEADSNFV LTNVGDIVEI QATGEKRGFT RGEFEQLFAL AEKGIGELFV KQLEAVSAAK PR

References

[1]

"Complete sequence of chromosome of Caulobacter sp. K31." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Bruce D., Goodwin L., Thompson L.S., Brettin T., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Kim E., Stephens C., Richardson P. Submitted (JAN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K31.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000927 Genomic DNA. Translation: ABZ69304.1.
RefSeq	YP_001681802.1. NC_010338.1.
3D structure databases
ProteinModelPortal	B0T369.
SMR	B0T369. Positions 2-237.
ModBase	Search...
Protein-protein interaction databases
STRING	B0T369.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5897879.
GenomeReviews	Gene locus Caul_0167 in contig CP000927_GR.
KEGG	cak:Caul_0167.
PATRIC	21313350. VBICauSp18104_0548.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG737187.
OMA	MLPRATG.
ProtClustDB	PRK00173.
Enzyme and pathway databases
BioCyc	CSP78:CAUL_0167-MONOMER.
Family and domain databases
HAMAP	MF_00564. RNase_PH. [Tree]
InterPro	IPR001247. ExoRNase_PH_dom1. IPR015847. ExoRNase_PH_dom2. IPR020568. Ribosomal_S5_D2-typ_fold. IPR002381. RNase_PH_bac-type. IPR018336. RNase_PH_CS. [Graphical view]
KO	K00989.
Pfam	PF01138. RNase_PH. 1 hit. PF03725. RNase_PH_C. 1 hit. [Graphical view]
SUPFAM	SSF55666. 3_ExoRNase. 1 hit. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR01966. RNasePH. 1 hit.
PROSITE	PS01277. RIBONUCLEASE_PH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNPH_CAUSK

Accession

Primary (citable) accession number: B0T369

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	April 8, 2008
Last modified:	January 25, 2012
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program