Reviewed, UniProtKB/Swiss-Prot B0T315 (PROA_CAUSK)

Names and origin

Protein names

Recommended name:
    Gamma-glutamyl phosphate reductase
      Short name=GPR
    EC=1.2.1.41
Alternative name(s):
    Glutamate-5-semialdehyde dehydrogenase
    Glutamyl-gamma-semialdehyde dehydrogenase
      Short name=GSA dehydrogenase

Gene names

Name:	proA
Ordered Locus Names:	Caul_4717

Organism

Caulobacter sp. (strain K31) [Complete proteome] [HAMAP]

Taxonomic identifier

366602 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Caulobacterales › Caulobacteraceae › Caulobacter

Protein attributes

Sequence length	419 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate By similarity.
Catalytic activity	L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH. HAMAP MF_00412
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2. HAMAP MF_00412
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the gamma-glutamyl phosphate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW proline biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADP or NADPH binding Inferred from electronic annotation. Source: InterPro glutamate-5-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 419	419	Gamma-glutamyl phosphate reductase HAMAP MF_00412		PRO_0000340876

Sequences

	Sequence		Length	Mass (Da)
	B0T315-1 [UniParc]. Last modified June 10, 2008. Version 2. Checksum: D91003013D3509AB	FASTA	419	43,111
	10 20 30 40 50 60 MSLQATMTAM GQAARQGASA LRVATPAQRT AALHAMAAAI RADAPAILAA NAKDLEKAGA 70 80 90 100 110 120 GGLTAPMVER LMLNPERLED VAAGVEAVAA IPDPLGVETA RWTRPNGLDI ARVRTPIGVI 130 140 150 160 170 180 AMIFESRPNV TADAAALCVR SGNAVILRGG SECIHSNLAI HAAIAKGLKA AGISSDAVQI 190 200 210 220 230 240 VRTPDRDAVG AILGGLNRTI DLIIPRGGKS LVARVQAEAR VAVLGHLEGL NHVFVHAAAD 250 260 270 280 290 300 PRKAVEIVLN AKMRRVSVCG SAETLLVDRA AAERLLPPIA DALIKAGCEL RGDGPSRAIE 310 320 330 340 350 360 PTMKPAVEAD WSTEYLAPVI SVAVVDGVEG AAAHIAAYGS GHTDAIVTED VAAAERFIAL 370 380 390 400 410 VDSAIVLVNA STQFADGGEF GFGAEIGIAT DKLHARGPVG AEQLTTFKYV VRGTGQTRP

References

[1]

"Complete sequence of chromosome of Caulobacter sp. K31." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Bruce D., Goodwin L., Thompson L.S., Brettin T., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Kim E., Stephens C., Richardson P. Submitted (JAN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000927 Genomic DNA. Translation: ABZ73837.1. Different initiation.
RefSeq	YP_001686335.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5902179.
GenomeReviews	Gene locus Caul_4717 in contig CP000927_GR.
KEGG	cak:Caul_4717.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00412. [Tree]
InterPro	IPR016163. Ald_DH_C. IPR016162. Ald_DH_N. IPR000965. Gglut_pp_reduct. IPR012134. Glu-5-SA_DH. [Graphical view]
Gene3D	G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PIRSF	PIRSF000151. GPR. 1 hit.
TIGRFAMs	TIGR00407. proA. 1 hit.
PROSITE	PS01223. PROA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROA_CAUSK

Accession

Primary (citable) accession number: B0T315

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	June 10, 2008
Last modified:	June 16, 2009
This is version 11 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)