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B0T280

- B0T280_CAUSK

UniProt

B0T280 - B0T280_CAUSK

Protein

Acetyl-coenzyme A synthetase

Gene

acsA

Organism
Caulobacter sp. (strain K31)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 46 (01 Oct 2014)
      Sequence version 1 (08 Apr 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA.UniRule annotation

    Catalytic activityi

    ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA.UniRule annotation

    Cofactori

    Magnesium.UniRule annotationSAAS annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei309 – 3091Coenzyme AUniRule annotation
    Binding sitei333 – 3331Coenzyme AUniRule annotation
    Binding sitei385 – 3851Substrate; via nitrogen amideUniRule annotation
    Binding sitei498 – 4981SubstrateUniRule annotation
    Binding sitei513 – 5131SubstrateUniRule annotation
    Active sitei515 – 5151UniRule annotation
    Binding sitei521 – 5211Coenzyme AUniRule annotation
    Binding sitei524 – 5241SubstrateUniRule annotation
    Metal bindingi535 – 5351Magnesium; via carbonyl oxygenUniRule annotation
    Metal bindingi537 – 5371Magnesium; via carbonyl oxygenUniRule annotation
    Metal bindingi540 – 5401Magnesium; via carbonyl oxygenUniRule annotation
    Binding sitei582 – 5821Coenzyme AUniRule annotation

    GO - Molecular functioni

    1. acetate-CoA ligase activity Source: UniProtKB-HAMAP
    2. AMP binding Source: InterPro
    3. ATP binding Source: UniProtKB-KW
    4. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. acetyl-CoA biosynthetic process from acetate Source: InterPro

    Keywords - Molecular functioni

    LigaseUniRule annotationImported

    Keywords - Ligandi

    ATP-bindingUniRule annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciCSP366602:GH0Y-112-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Acetyl-coenzyme A synthetaseUniRule annotation (EC:6.2.1.1UniRule annotation)
    Short name:
    AcCoA synthetaseUniRule annotation
    Short name:
    AcsUniRule annotation
    Alternative name(s):
    Acetate--CoA ligaseUniRule annotation
    Acyl-activating enzymeUniRule annotation
    Gene namesi
    Name:acsAUniRule annotation
    Ordered Locus Names:Caul_0111Imported
    OrganismiCaulobacter sp. (strain K31)Imported
    Taxonomic identifieri366602 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaCaulobacteralesCaulobacteraceaeCaulobacter
    ProteomesiUP000001316: Chromosome

    PTM / Processingi

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei607 – 6071N6-acetyllysineUniRule annotation

    Post-translational modificationi

    Acetylated. Deacetylation by the SIR2-homolog deacetylase activates the enzyme.UniRule annotation

    Keywords - PTMi

    AcetylationUniRule annotation

    Interactioni

    Protein-protein interaction databases

    STRINGi366602.Caul_0111.

    Structurei

    3D structure databases

    ProteinModelPortaliB0T280.
    SMRiB0T280. Positions 9-643.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni409 – 4146Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the ATP-dependent AMP-binding enzyme family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0365.
    HOGENOMiHOG000229981.
    KOiK01895.
    OMAiINDAEAC.
    OrthoDBiEOG68WR2H.

    Family and domain databases

    HAMAPiMF_01123. Ac_CoA_synth.
    InterProiIPR011904. Ac_CoA_lig.
    IPR025110. AMP-bd_C.
    IPR020845. AMP-binding_CS.
    IPR000873. AMP-dep_Synth/Lig.
    [Graphical view]
    PfamiPF00501. AMP-binding. 1 hit.
    PF13193. AMP-binding_C. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR02188. Ac_CoA_lig_AcsA. 1 hit.
    PROSITEiPS00455. AMP_BINDING. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B0T280-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSDGQVFPIP DDWAGKAHID AAGYEAALAR VEADPEGYWR DLAGRLDWIR    50
    APTQIKDVSF AKDDFRIHWY ADGVLNVSAN CIDRHLPHRK DEVALIFEGD 100
    EPGVSSTLTY GQLHEEVCRM ANVLKVQGVR KGDRVTIYLP MIPIAAVAML 150
    ACARIGAVHS VVFGGFSPDS IAGRIQDCGS HFVITADEGR RGGKRVPLKA 200
    NIDKALEHCP WVSKVLMVRW TGAETTLVRH RDVVWEDVKD SVSADCPPEP 250
    MNAEDPLFIL YTSGSTGKPK GVLHTTGGYL AWAAWTHEAV FDYRPGEVFW 300
    CTADVGWVTG HSYVVYGPLA NGGTSLIFEG VPNYPTPARF WEVVDKHQVE 350
    IFYTAPTALR ALMREGDDWV TKNDLSSLRL LGSVGEPINP EAWLWYHRVV 400
    GKDRLPIVDT WWQTETGGVL VSPLPGATAL KPGSATKPLP GVKLQLVDAE 450
    GHVLEGATEG NLLITDSWPG QMRTVYGDHG RFFDTYFSAY PGKYFTGDGC 500
    RRDADGYYWI TGRVDDVINV SGHRLGTAEI ESALVAHDTV AEAAVVGYPH 550
    DIKGQGVYAY VTLRAGVEPT DSLRKDLMLW VRQEIGPFAA PDVLQWAPGL 600
    PKTRSGKIMR RILRKIAENE LGSLGDISTL ADPSVVDDLV KNRANS 646
    Length:646
    Mass (Da):70,982
    Last modified:April 8, 2008 - v1
    Checksum:i1AA950EAE622162D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000927 Genomic DNA. Translation: ABZ69249.1.
    RefSeqiWP_012284207.1. NC_010338.1.
    YP_001681747.1. NC_010338.1.

    Genome annotation databases

    EnsemblBacteriaiABZ69249; ABZ69249; Caul_0111.
    GeneIDi5897823.
    KEGGicak:Caul_0111.
    PATRICi21313238. VBICauSp18104_0493.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000927 Genomic DNA. Translation: ABZ69249.1 .
    RefSeqi WP_012284207.1. NC_010338.1.
    YP_001681747.1. NC_010338.1.

    3D structure databases

    ProteinModelPortali B0T280.
    SMRi B0T280. Positions 9-643.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 366602.Caul_0111.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABZ69249 ; ABZ69249 ; Caul_0111 .
    GeneIDi 5897823.
    KEGGi cak:Caul_0111.
    PATRICi 21313238. VBICauSp18104_0493.

    Phylogenomic databases

    eggNOGi COG0365.
    HOGENOMi HOG000229981.
    KOi K01895.
    OMAi INDAEAC.
    OrthoDBi EOG68WR2H.

    Enzyme and pathway databases

    BioCyci CSP366602:GH0Y-112-MONOMER.

    Family and domain databases

    HAMAPi MF_01123. Ac_CoA_synth.
    InterProi IPR011904. Ac_CoA_lig.
    IPR025110. AMP-bd_C.
    IPR020845. AMP-binding_CS.
    IPR000873. AMP-dep_Synth/Lig.
    [Graphical view ]
    Pfami PF00501. AMP-binding. 1 hit.
    PF13193. AMP-binding_C. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR02188. Ac_CoA_lig_AcsA. 1 hit.
    PROSITEi PS00455. AMP_BINDING. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K31Imported.

    Entry informationi

    Entry nameiB0T280_CAUSK
    AccessioniPrimary (citable) accession number: B0T280
    Entry historyi
    Integrated into UniProtKB/TrEMBL: April 8, 2008
    Last sequence update: April 8, 2008
    Last modified: October 1, 2014
    This is version 46 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteomeImported

    External Data

    Dasty 3