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B0T280

- B0T280_CAUSK

UniProt

B0T280 - B0T280_CAUSK

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Protein

Acetyl-coenzyme A synthetase

Gene

acsA

Organism
Caulobacter sp. (strain K31)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA.UniRule annotation

Catalytic activityi

ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA.UniRule annotation

Cofactori

Note: Magnesium.UniRule annotationSAAS annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei309 – 3091Coenzyme AUniRule annotation
Binding sitei333 – 3331Coenzyme AUniRule annotation
Binding sitei498 – 4981ATPUniRule annotation
Binding sitei513 – 5131ATPUniRule annotation
Binding sitei521 – 5211Coenzyme A; via carbonyl oxygenUniRule annotation
Binding sitei524 – 5241ATPUniRule annotation
Metal bindingi535 – 5351Magnesium; via carbonyl oxygenUniRule annotation
Metal bindingi537 – 5371Magnesium; via carbonyl oxygenUniRule annotation
Metal bindingi540 – 5401Magnesium; via carbonyl oxygenUniRule annotation
Binding sitei582 – 5821Coenzyme AUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi385 – 3873ATPUniRule annotation
Nucleotide bindingi409 – 4146ATPUniRule annotation

GO - Molecular functioni

  1. acetate-CoA ligase activity Source: UniProtKB-HAMAP
  2. AMP binding Source: InterPro
  3. ATP binding Source: UniProtKB-KW
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. acetyl-CoA biosynthetic process from acetate Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

LigaseUniRule annotationImported

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciCSP366602:GH0Y-112-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetyl-coenzyme A synthetaseUniRule annotation (EC:6.2.1.1UniRule annotation)
Short name:
AcCoA synthetaseUniRule annotation
Short name:
AcsUniRule annotation
Alternative name(s):
Acetate--CoA ligaseUniRule annotation
Acyl-activating enzymeUniRule annotation
Gene namesi
Name:acsAUniRule annotation
Ordered Locus Names:Caul_0111Imported
OrganismiCaulobacter sp. (strain K31)Imported
Taxonomic identifieri366602 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaCaulobacteralesCaulobacteraceaeCaulobacter
ProteomesiUP000001316: Chromosome

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei607 – 6071N6-acetyllysineUniRule annotation

Post-translational modificationi

Acetylated. Deacetylation by the SIR2-homolog deacetylase activates the enzyme.UniRule annotation

Keywords - PTMi

AcetylationUniRule annotation

Interactioni

Protein-protein interaction databases

STRINGi366602.Caul_0111.

Structurei

3D structure databases

ProteinModelPortaliB0T280.
SMRiB0T280. Positions 9-643.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni191 – 1944Coenzyme A bindingUniRule annotation

Sequence similaritiesi

Belongs to the ATP-dependent AMP-binding enzyme family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0365.
HOGENOMiHOG000229981.
KOiK01895.
OMAiINDAEAC.
OrthoDBiEOG68WR2H.

Family and domain databases

HAMAPiMF_01123. Ac_CoA_synth.
InterProiIPR011904. Ac_CoA_lig.
IPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B0T280-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSDGQVFPIP DDWAGKAHID AAGYEAALAR VEADPEGYWR DLAGRLDWIR
60 70 80 90 100
APTQIKDVSF AKDDFRIHWY ADGVLNVSAN CIDRHLPHRK DEVALIFEGD
110 120 130 140 150
EPGVSSTLTY GQLHEEVCRM ANVLKVQGVR KGDRVTIYLP MIPIAAVAML
160 170 180 190 200
ACARIGAVHS VVFGGFSPDS IAGRIQDCGS HFVITADEGR RGGKRVPLKA
210 220 230 240 250
NIDKALEHCP WVSKVLMVRW TGAETTLVRH RDVVWEDVKD SVSADCPPEP
260 270 280 290 300
MNAEDPLFIL YTSGSTGKPK GVLHTTGGYL AWAAWTHEAV FDYRPGEVFW
310 320 330 340 350
CTADVGWVTG HSYVVYGPLA NGGTSLIFEG VPNYPTPARF WEVVDKHQVE
360 370 380 390 400
IFYTAPTALR ALMREGDDWV TKNDLSSLRL LGSVGEPINP EAWLWYHRVV
410 420 430 440 450
GKDRLPIVDT WWQTETGGVL VSPLPGATAL KPGSATKPLP GVKLQLVDAE
460 470 480 490 500
GHVLEGATEG NLLITDSWPG QMRTVYGDHG RFFDTYFSAY PGKYFTGDGC
510 520 530 540 550
RRDADGYYWI TGRVDDVINV SGHRLGTAEI ESALVAHDTV AEAAVVGYPH
560 570 580 590 600
DIKGQGVYAY VTLRAGVEPT DSLRKDLMLW VRQEIGPFAA PDVLQWAPGL
610 620 630 640
PKTRSGKIMR RILRKIAENE LGSLGDISTL ADPSVVDDLV KNRANS
Length:646
Mass (Da):70,982
Last modified:April 8, 2008 - v1
Checksum:i1AA950EAE622162D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000927 Genomic DNA. Translation: ABZ69249.1.
RefSeqiWP_012284207.1. NC_010338.1.
YP_001681747.1. NC_010338.1.

Genome annotation databases

EnsemblBacteriaiABZ69249; ABZ69249; Caul_0111.
GeneIDi5897823.
KEGGicak:Caul_0111.
PATRICi21313238. VBICauSp18104_0493.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000927 Genomic DNA. Translation: ABZ69249.1 .
RefSeqi WP_012284207.1. NC_010338.1.
YP_001681747.1. NC_010338.1.

3D structure databases

ProteinModelPortali B0T280.
SMRi B0T280. Positions 9-643.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 366602.Caul_0111.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABZ69249 ; ABZ69249 ; Caul_0111 .
GeneIDi 5897823.
KEGGi cak:Caul_0111.
PATRICi 21313238. VBICauSp18104_0493.

Phylogenomic databases

eggNOGi COG0365.
HOGENOMi HOG000229981.
KOi K01895.
OMAi INDAEAC.
OrthoDBi EOG68WR2H.

Enzyme and pathway databases

BioCyci CSP366602:GH0Y-112-MONOMER.

Family and domain databases

HAMAPi MF_01123. Ac_CoA_synth.
InterProi IPR011904. Ac_CoA_lig.
IPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view ]
Pfami PF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITEi PS00455. AMP_BINDING. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K31Imported.

Entry informationi

Entry nameiB0T280_CAUSK
AccessioniPrimary (citable) accession number: B0T280
Entry historyi
Integrated into UniProtKB/TrEMBL: April 8, 2008
Last sequence update: April 8, 2008
Last modified: November 26, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3