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B0T162 (TRMB_CAUSK) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 41. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:Caul_0028
OrganismCaulobacter sp. (strain K31) [Complete proteome] [HAMAP]
Taxonomic identifier366602 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaCaulobacteralesCaulobacteraceaeCaulobacter

Protein attributes

Sequence length226 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 226226tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_1000084437

Regions

Region139 – 1446Interaction with RNA Potential
Region206 – 2094Substrate binding By similarity

Sites

Active site1331 By similarity
Binding site591S-adenosyl-L-methionine By similarity
Binding site841S-adenosyl-L-methionine By similarity
Binding site1111S-adenosyl-L-methionine By similarity
Binding site1331S-adenosyl-L-methionine By similarity
Binding site1371Substrate By similarity
Binding site1691Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
B0T162 [UniParc].

Last modified April 8, 2008. Version 1.
Checksum: A5F8BF67AFEE04D3

FASTA22625,360
        10         20         30         40         50         60 
MTHPQQPHGP LRSFGRLKSR PVKPRQQALL DTLLPQIAVP TAPFQPRDLM PEAQEVWLEI 

        70         80         90        100        110        120 
GFGGGEHMAA QAGKRPDVLM IGAEPFVNGV ASAVRHVEEQ ALKNVRIHEG DARDVVGWLP 

       130        140        150        160        170        180 
DASIDRLFIM FPDPWHKARH NKRRLVQPAF VAELARVLKP GAGFRFATDW ADYAQWTVER 

       190        200        210        220 
VLADPAFRFA DAEAVRNAPP ADHVTTRYEE KKLGDCAPVF LDFVRI 

« Hide

References

[1]"Complete sequence of chromosome of Caulobacter sp. K31."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Bruce D., Goodwin L., Thompson L.S., Brettin T., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L. expand/collapse author list , Kyrpides N., Kim E., Stephens C., Richardson P.
Submitted (JAN-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K31.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000927 Genomic DNA. Translation: ABZ69166.1.
RefSeqYP_001681664.1. NC_010338.1.

3D structure databases

ProteinModelPortalB0T162.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING366602.Caul_0028.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABZ69166; ABZ69166; Caul_0028.
GeneID5897740.
KEGGcak:Caul_0028.
PATRIC21313062. VBICauSp18104_0406.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000073969.
KOK03439.
OMAFERRSHR.
OrthoDBEOG6K6VBC.
ProtClustDBPRK00121.

Enzyme and pathway databases

BioCycCSP366602:GH0Y-28-MONOMER.
UniPathwayUPA00989.

Family and domain databases

HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_CAUSK
AccessionPrimary (citable) accession number: B0T162
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: April 8, 2008
Last modified: April 16, 2014
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways