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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Caulobacter sp. (strain K31)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathway: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei59 – 591S-adenosyl-L-methionineUniRule annotation
Binding sitei84 – 841S-adenosyl-L-methionineUniRule annotation
Binding sitei111 – 1111S-adenosyl-L-methionineUniRule annotation
Active sitei133 – 1331By similarity
Binding sitei133 – 1331S-adenosyl-L-methionineUniRule annotation
Binding sitei137 – 1371SubstrateUniRule annotation
Binding sitei169 – 1691SubstrateUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciCSP366602:GH0Y-28-MONOMER.
UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:Caul_0028
OrganismiCaulobacter sp. (strain K31)
Taxonomic identifieri366602 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaCaulobacteralesCaulobacteraceaeCaulobacter
ProteomesiUP000001316 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 226226tRNA (guanine-N(7)-)-methyltransferasePRO_1000084437Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi366602.Caul_0028.

Structurei

3D structure databases

ProteinModelPortaliB0T162.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni139 – 1446Interaction with RNAUniRule annotation
Regioni206 – 2094Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000073969.
KOiK03439.
OMAiYICHLAR.
OrthoDBiEOG6K6VBC.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B0T162-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTHPQQPHGP LRSFGRLKSR PVKPRQQALL DTLLPQIAVP TAPFQPRDLM
60 70 80 90 100
PEAQEVWLEI GFGGGEHMAA QAGKRPDVLM IGAEPFVNGV ASAVRHVEEQ
110 120 130 140 150
ALKNVRIHEG DARDVVGWLP DASIDRLFIM FPDPWHKARH NKRRLVQPAF
160 170 180 190 200
VAELARVLKP GAGFRFATDW ADYAQWTVER VLADPAFRFA DAEAVRNAPP
210 220
ADHVTTRYEE KKLGDCAPVF LDFVRI
Length:226
Mass (Da):25,360
Last modified:April 8, 2008 - v1
Checksum:iA5F8BF67AFEE04D3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000927 Genomic DNA. Translation: ABZ69166.1.
RefSeqiWP_012284124.1. NC_010338.1.
YP_001681664.1. NC_010338.1.

Genome annotation databases

EnsemblBacteriaiABZ69166; ABZ69166; Caul_0028.
KEGGicak:Caul_0028.
PATRICi21313062. VBICauSp18104_0406.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000927 Genomic DNA. Translation: ABZ69166.1.
RefSeqiWP_012284124.1. NC_010338.1.
YP_001681664.1. NC_010338.1.

3D structure databases

ProteinModelPortaliB0T162.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi366602.Caul_0028.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABZ69166; ABZ69166; Caul_0028.
KEGGicak:Caul_0028.
PATRICi21313062. VBICauSp18104_0406.

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000073969.
KOiK03439.
OMAiYICHLAR.
OrthoDBiEOG6K6VBC.

Enzyme and pathway databases

UniPathwayiUPA00989.
BioCyciCSP366602:GH0Y-28-MONOMER.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K31.

Entry informationi

Entry nameiTRMB_CAUSK
AccessioniPrimary (citable) accession number: B0T162
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: April 8, 2008
Last modified: April 1, 2015
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.