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B0SZT0 (BIOD_CAUSK) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 28. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
ATP-dependent dethiobiotin synthetase BioD
EC=6.3.3.3
Alternative name(s):
DTB synthetase
Short name=DTBS
Dethiobiotin synthase
Gene names
Name:bioD
Ordered Locus Names:Caul_2876
OrganismCaulobacter sp. (strain K31) [Complete proteome] [HAMAP]
Taxonomic identifier366602 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Caulobacterales › Caulobacteraceae › Caulobacter

Protein attributes

Sequence length230 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes a mechanistically unusual reaction, the ATP-dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8-diaminopelargonic acid (DAPA) to form an ureido ring By similarity. HAMAP MF_00336

Catalytic activity

ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin. HAMAP MF_00336

Cofactor

Magnesium By similarity. HAMAP MF_00336

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2. HAMAP MF_00336

Subcellular location

Cytoplasm By similarity HAMAP MF_00336.

Sequence similarities

Belongs to the dethiobiotin synthetase family.

Ontologies

Keywords
   Biological processBiotin biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Magnesium
Metal-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processbiotin biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

dethiobiotin synthase activity

Inferred from electronic annotation. Source: EC

magnesium ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 230230ATP-dependent dethiobiotin synthetase BioD HAMAP MF_00336
PRO_1000079271

Regions

Nucleotide binding115 – 1184ATP By similarity
Nucleotide binding175 – 1762ATP By similarity

Sites

Metal binding121Magnesium 1 By similarity
Metal binding161Magnesium 2 By similarity
Metal binding521Magnesium 2 By similarity
Metal binding1151Magnesium 2 By similarity
Binding site411Substrate By similarity
Binding site521ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B0SZT0 [UniParc].

Last modified April 8, 2008. Version 1.
Checksum: 75B8F1DE5D1B2D00

FASTA23023,474
        10         20         30         40         50         60 
MPALFVTGTG TDIGKTHVSC ALIRALKVRG AVVDAFKPVV SGFDPKDAAG SDPARLAIAL 

        70         80         90        100        110        120 
GDPSAVFRIA PRRYRAPLSP NIAAQLEGET LVLDDMVIDA VARAAELRDG LLLVEGAGGV 

       130        140        150        160        170        180 
MSPLTDTQTN LDMIVALGAP VLLVAGSYLG TISHVLTALV ALRAAKVRVA AVVISESLDA 

       190        200        210        220        230 
PDLDQTAQAL APFLDGAPLF LAPRGESWDA GALADHLLAA VAPNAFGGTP

« Hide

References

[1]"Complete sequence of chromosome of Caulobacter sp. K31."
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Bruce D., Goodwin L., Thompson L.S., Brettin T., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L. expand/collapse author list , Kyrpides N., Kim E., Stephens C., Richardson P.
Submitted (JAN-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K31.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000927 Genomic DNA. Translation: ABZ72003.1.
RefSeqYP_001684501.1. NC_010338.1.

3D structure databases

ProteinModelPortalB0SZT0.
ModBaseSearch...

Protein-protein interaction databases

STRINGB0SZT0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID5900331.
GenomeReviewsGene locus Caul_2876 in contig CP000927_GR.
KEGGcak:Caul_2876.
PATRIC21318846. VBICauSp18104_3275.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG650065.
OMAPQMERIE.
ProtClustDBCLSK890484.

Enzyme and pathway databases

BioCycCSP78:CAUL_2876-MONOMER.

Family and domain databases

HAMAPMF_00336. BioD.
[Tree]
InterProIPR002586. CbiA_P_synth.
IPR004472. DTB_synth_BioD.
[Graphical view]
KOK01935.
PfamPF01656. CbiA. 1 hit.
[Graphical view]
PIRSFPIRSF006755. DTB_synth. 1 hit.
TIGRFAMsTIGR00347. BioD. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOD_CAUSK
AccessionPrimary (citable) accession number: B0SZT0
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: April 8, 2008
Last modified: January 25, 2012
This is version 28 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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